Threonylcarbamoyl-AMP synthase (P45748)

Identification
Name:Threonylcarbamoyl-AMP synthase
Synonyms:Not Available
Gene Name:tsaC
Enzyme Class:
Biological Properties
General Function:threonylcarbamoyladenosine biosynthetic process
Specific Function:Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, bicarbonate/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of pyrophosphate. Is also able to catalyze the reverse reaction in vitro, i.e. the formation of ATP from TC-AMP and PPi. Shows higher affinity for the full-length tRNA(Thr) lacking only the t(6)A37 modification than for its fully modified counterpart. Could also be required for the maturation of 16S rRNA. Binds to double-stranded RNA but does not interact tightly with either of the ribosomal subunits, or the 70S particles.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Threonine + 1.0Adenosine triphosphate + 1.0Hydrogen carbonate ↔ 1.0L-Threonylcarbamoyladenylate + 1.0Pyrophosphate + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB00595Hydrogen carbonateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00167L-ThreonineMetaboCard
ECMDB23796L-ThreonylcarbamoyladenylateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
ATP binding
cytoplasm
double-stranded RNA binding
nucleotidyltransferase activity
regulation of translational fidelity
rRNA processing
threonylcarbamoyladenosine biosynthetic process
tRNA binding
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>573
gtgaataataacctgcaaagagacgctatcgcagctgcgatagatgttctcaatgaagaa
cgtgtcatcgcctatccaacggaagccgttttcggtgttgggtgcgatcctgatagcgaa
acagcagtgatgcgactgttggagttaaaacagcgtccggttgataaggggctgatttta
atcgcagcaaattacgagcagcttaaaccctatattgatgacaccatgttgactgacgtg
cagcgtgaaaccattttttcccgctggccaggtcctgtcacctttgtctttcccgcgcct
gcgacaacaccgcgctggttgacgggccgctttgattcgcttgctgtacgagtcaccgac
catccgttggtggttgctttgtgccaggcttatggtaaaccgctggtttctaccagtgcc
aacttgagtggattgccaccttgtcgaacagtagacgaagttcgcgcacaatttggcgcg
gcgttcccggttgtgcctggtgaaacgggggggcgtttaaatccttcagaaatccgcgat
gccctgacgggtgaactgtttcgacaggggtaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:190
Protein Molecular Weight:20767
Protein Theoretical pI:Not Available
PDB File:1HRU
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>Threonylcarbamoyl-AMP synthase
MNNNLQRDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVMRLLELKQRPVDKGLIL
IAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPATTPRWLTGRFDSLAVRVTD
HPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGAAFPVVPGETGGRLNPSEIRD
ALTGELFRQG
References
External Links:
ResourceLink
Uniprot ID:P45748
Uniprot Name:TSAC_ECOLI
PDB ID:1HRU
CCDB:TSAC_ECOLI
General Reference:Not Available