| Identification |
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| Name: | Threonylcarbamoyl-AMP synthase |
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| Synonyms: | Not Available |
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| Gene Name: | tsaC |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | threonylcarbamoyladenosine biosynthetic process |
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| Specific Function: | Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, bicarbonate/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of pyrophosphate. Is also able to catalyze the reverse reaction in vitro, i.e. the formation of ATP from TC-AMP and PPi. Shows higher affinity for the full-length tRNA(Thr) lacking only the t(6)A37 modification than for its fully modified counterpart. Could also be required for the maturation of 16S rRNA. Binds to double-stranded RNA but does not interact tightly with either of the ribosomal subunits, or the 70S particles. |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| KEGG Reactions: | |
1.0 | + | 1.0 | + | 1.0 | ↔ | 1.0 | + | 1.0 | + | 1.0 |
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| Metabolites: | |
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| GO Classification: | | Function |
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| ATP binding | | cytoplasm | | double-stranded RNA binding | | nucleotidyltransferase activity | | regulation of translational fidelity | | rRNA processing | | threonylcarbamoyladenosine biosynthetic process | | tRNA binding |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >573
gtgaataataacctgcaaagagacgctatcgcagctgcgatagatgttctcaatgaagaa
cgtgtcatcgcctatccaacggaagccgttttcggtgttgggtgcgatcctgatagcgaa
acagcagtgatgcgactgttggagttaaaacagcgtccggttgataaggggctgatttta
atcgcagcaaattacgagcagcttaaaccctatattgatgacaccatgttgactgacgtg
cagcgtgaaaccattttttcccgctggccaggtcctgtcacctttgtctttcccgcgcct
gcgacaacaccgcgctggttgacgggccgctttgattcgcttgctgtacgagtcaccgac
catccgttggtggttgctttgtgccaggcttatggtaaaccgctggtttctaccagtgcc
aacttgagtggattgccaccttgtcgaacagtagacgaagttcgcgcacaatttggcgcg
gcgttcccggttgtgcctggtgaaacgggggggcgtttaaatccttcagaaatccgcgat
gccctgacgggtgaactgtttcgacaggggtaa |
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 190 |
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| Protein Molecular Weight: | 20767 |
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| Protein Theoretical pI: | Not Available |
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| PDB File: | 1HRU |
| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >Threonylcarbamoyl-AMP synthase
MNNNLQRDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVMRLLELKQRPVDKGLIL
IAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPATTPRWLTGRFDSLAVRVTD
HPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGAAFPVVPGETGGRLNPSEIRD
ALTGELFRQG |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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