UDP-2,3-diacylglucosamine hydrolase (P43341)

Identification
Name:UDP-2,3-diacylglucosamine hydrolase
Synonyms:Not Available
Gene Name:lpxH
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity
Specific Function:Catalyzes the hydrolysis of the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1- phosphate (lipid X) and UMP
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 2.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine ↔ 2.0Hydrogen ion + 1.02,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + 1.0Uridine 5'-monophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine + 1.0Water ↔ 1.0Uridine 5'-monophosphate + 1.02,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine + 1.0Water → 1.0Uridine 5'-monophosphate + 1.0Hydrogen ion + 1.02,3-bis[(3R)-3-hydroxymyristoyl]-α-D-glucosaminyl 1-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 2.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine ↔ 2.0Hydrogen ion + 1.02,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + 1.0Uridine 5'-monophosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine → 1.0Hydrogen ion + 1.02,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + 1.0Uridine 5'-monophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB232402,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphateMetaboCard
ECMDB200542,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphateMetaboCard
ECMDB242172,3-bis[(3R)-3-hydroxymyristoyl]-α-D-glucosaminyl 1-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB20201UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamineMetaboCard
ECMDB24216UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamineMetaboCard
ECMDB00288Uridine 5'-monophosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
pyrophosphatase activity
Process
lipid A biosynthetic process
metabolic process
organophosphate metabolic process
phospholipid biosynthetic process
phospholipid metabolic process
Gene Properties
Blattner:b0524
Gene OrientationCounterclockwise
Centisome Percentage:11.91
Left Sequence End552441
Right Sequence End553163
Gene Sequence:
>723 bp
ATGGCGACACTCTTTATTGCAGATCTTCATCTCTGCGTGGAAGAACCGGCGATCACCGCC
GGTTTTCTGCGTTTTTTAGCGGGGGAAGCCCGCAAGGCCGACGCGCTGTATATTCTTGGC
GATCTGTTTGAAGCATGGATTGGCGACGACGATCCCAACCCACTCCATCGCAAGATGGCG
GCGGCGATCAAAGCGGTGTCCGATTCCGGCGTTCCCTGTTATTTCATTCATGGCAACCGT
GATTTTCTGCTCGGCAAACGCTTTGCCCGTGAAAGTGGCATGACGTTATTGCCGGAAGAA
AAGGTGCTCGAACTTTATGGTCGCCGGGTGTTGATTATGCATGGCGACACGCTGTGCACC
GATGACGCGGGTTATCAGGCTTTTCGCGCCAAGGTCCACAAACCCTGGCTGCAGACGCTA
TTCCTCGCCCTGCCGTTGTTTGTGCGCAAACGCATTGCCGCGCGAATGCGCGCGAACAGC
AAAGAAGCCAACAGCAGTAAATCGCTGGCGATCATGGACGTTAACCAAAACGCGGTGGTC
AGTGCGATGGAAAAACATCAGGTGCAATGGCTGATCCACGGGCATACCCATCGCCCGGCG
GTGCATGAACTTATCGCCAATCAGCAACCTGCTTTTCGCGTGGTACTGGGTGCCTGGCAT
ACGGAAGGTTCAATGGTGAAAGTCACGGCGGATGACGTTGAGCTGATTCATTTTCCGTTT
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:240
Protein Molecular Weight:26894
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>UDP-2,3-diacylglucosamine hydrolase
MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFEAWIGDDDPNPLHRKMA
AAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKVLELYGRRVLIMHGDTLCT
DDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANSKEANSSKSLAIMDVNQNAVV
SAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGAWHTEGSMVKVTADDVELIHFPF
References
External Links:
ResourceLink
Uniprot ID:P43341
Uniprot Name:LPXH_ECOLI
GenBank Gene ID:AF311865
Genebank Protein ID:12044356
Ecogene ID:EG12666
Ecocyc:EG12666
ColiBase:b0524
Kegg Gene:b0524
EchoBASE ID:EB2532
CCDB:LPXH_ECOLI
BacMap:16128508
General Reference:
  • Babinski, K. J., Kanjilal, S. J., Raetz, C. R. (2002). "Accumulation of the lipid A precursor UDP-2,3-diacylglucosamine in an Escherichia coli mutant lacking the lpxH gene." J Biol Chem 277:25947-25956. Pubmed: 12000771
  • Babinski, K. J., Ribeiro, A. A., Raetz, C. R. (2002). "The Escherichia coli gene encoding the UDP-2,3-diacylglucosamine pyrophosphatase of lipid A biosynthesis." J Biol Chem 277:25937-25946. Pubmed: 12000770
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., McIninch, J. D., Koonin, E. V., Rudd, K. E., Medigue, C., Danchin, A. (1995). "Detection of new genes in a bacterial genome using Markov models for three gene classes." Nucleic Acids Res 23:3554-3562. Pubmed: 7567469
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Watanabe, W., Sampei, G., Aiba, A., Mizobuchi, K. (1989). "Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis." J Bacteriol 171:198-204. Pubmed: 2644189