Pyrimidine-specific ribonucleoside hydrolase rihA (P41409)

Identification
Name:Pyrimidine-specific ribonucleoside hydrolase rihA
Synonyms:
  • Cytidine/uridine-specific hydrolase
Gene Name:rihA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in hydrolase activity, hydrolyzing N-glycosyl compounds
Specific Function:Hydrolyzes with equal efficiency cytidine or uridine to ribose and cytosine or uracil, respectively
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Cytidine + 1.0Water → 1.0Cytosine + 1.0Ribose
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0D-ribose+1.0Thumb
1.0Cytidine + 1.0Water → 1.0D-ribose + 1.0Cytosine
ReactionCard
1.0Thumb+1.0Thumb → 1.0D-ribose+1.0Thumb
1.0Uridine + 1.0Water → 1.0D-ribose + 1.0Uracil
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Water + 1.0Uridine → 1.0Ribose + 1.0Uracil
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00089CytidineMetaboCard
ECMDB00630CytosineMetaboCard
ECMDB00283RiboseMetaboCard
ECMDB00300UracilMetaboCard
ECMDB00296UridineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing N-glycosyl compounds
Gene Properties
Blattner:b0651
Gene OrientationCounterclockwise
Centisome Percentage:14.71
Left Sequence End682700
Right Sequence End683635
Gene Sequence:
>936 bp
ATGGCACTGCCAATTCTGTTAGATTGCGACCCAGGTCATGACGACGCTATCGCAATAGTT
CTCGCCCTCGCCTCACCAGAGCTTGATGTCAAAGCAATTACGTCTTCCGCCGGAAACCAG
ACACCAGAAAAAACCTTACGCAATGTTCTGCGTATGCTGACCTTGCTTAATCGCACCGAT
ATTCCGGTAGCAGGCGGCGCGGTAAAACCGTTAATGCGTGAGTTGATTATCGCGGACAAT
GTGCACGGCGAAAGCGGTCTCGACGGCCCGGCATTACCGGAACCGACATTCGCACCGCAA
AACTGTACGGCGGTAGAGCTGATGGCGAAAACGCTGCGTGAAAGTGCGGAACCTGTCACC
ATTGTGTCTACCGGACCGCAAACTAACGTTGCCTTGCTGCTCAATAGCCATCCGGAACTG
CATAGCAAAATTGCCCGTATCGTGATTATGGGTGGCGCTATGGGGCTGGGTAACTGGACG
CCTGCGGCTGAATTTAATATTTACGTTGACCCGGAAGCGGCAGAAATTGTCTTCCAGTCA
GGGATCCCGGTGGTGATGGCCGGTCTGGATGTTACTCATAAAGCACAAATCCACGTTGAA
GACACCGAGCGTTTCCGCGCGATTGGTAACCCTGTTTCAACCATTGTTGCCGAACTGCTG
GATTTCTTCCTCGAATATCATAAAGACGAAAAATGGGGCTTTGTCGGCGCACCACTGCAT
GACCCATGCACCATCGCCTGGCTGTTGAAACCGGAGTTATTTACCTCTGTTGAGCGCTGG
GTTGGCGTGGAAACACAGGGGAAATATACCCAGGGTATGACGGTTGTTGATTATTATTAT
CTGACAGGCAATAAACCGAATGCCACCGTAATGGTCGATGTTGATCGTCAGGGCTTTGTT
GATTTACTGGCTGATCGTCTGAAATTTTACGCTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:311
Protein Molecular Weight:33823
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Pyrimidine-specific ribonucleoside hydrolase rihA
MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEKTLRNVLRMLTLLNRTD
IPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNCTAVELMAKTLRESAEPVT
IVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWTPAAEFNIYVDPEAAEIVFQS
GIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAELLDFFLEYHKDEKWGFVGAPLH
DPCTIAWLLKPELFTSVERWVGVETQGKYTQGMTVVDYYYLTGNKPNATVMVDVDRQGFV
DLLADRLKFYA
References
External Links:
ResourceLink
Uniprot ID:P41409
Uniprot Name:RIHA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062271
Ecogene ID:EG12701
Ecocyc:EG12701
ColiBase:b0651
Kegg Gene:b0651
EchoBASE ID:EB2563
CCDB:RIHA_ECOLI
BacMap:16128634
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Petersen, C., Moller, L. B. (2001). "The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation." J Biol Chem 276:884-894. Pubmed: 11027694