Gnt-II system L-idonate transporter (P39344)

Identification
Name:Gnt-II system L-idonate transporter
Synonyms:
  • L-Ido transporter
  • 5-keto-D-gluconate transporter
Gene Name:idnT
Enzyme Class:Not Available
Biological Properties
General Function:Involved in gluconate transmembrane transporter activity
Specific Function:Transports L-idonate, D-gluconate and 5-keto-D- gluconate, from the periplasm across the inner membrane
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB117315-Keto-D-gluconateMetaboCard
ECMDB20140D-GalactonateMetaboCard
ECMDB00625Gluconic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21376L-IdonateMetaboCard
GO Classification:
Component
cell part
membrane
Function
carboxylic acid transmembrane transporter activity
gluconate transmembrane transporter activity
monocarboxylic acid transmembrane transporter activity
organic acid transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
carboxylic acid transport
establishment of localization
gluconate transport
monocarboxylic acid transport
organic acid transport
transport
Gene Properties
Blattner:b4265
Gene OrientationCounterclockwise
Centisome Percentage:96.76
Left Sequence End4489229
Right Sequence End4490548
Gene Sequence:
>1320 bp
ATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTG
TTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACAC
GTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTA
GTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTC
GCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCG
CGTACAGCTACCGTTTCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTG
CCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCG
GGCAAGCTGCTGGATACCGTGGGCAACTTCCTTGCGCCGCTGAAAATTATCGCGCTGGTC
ATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCT
TATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTG
GGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAA
GCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACT
CTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCT
GCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGC
TTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGT
GCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATC
CTCGGCGGCTTCTCGATGGTGGTTTCTAACCTCGGCTTAAGCCAGCTGATCCAGATCTCC
GTACCGGTGCTGACCGCTATTTATCCGCCGTGTATCGCACTGGTTGTATTAAGTTTTACA
CGCTCATGGTGGCATAATTCGTCCCGCGTGATTGCTCCGCCGATGTTTATCAGCCTGCTT
TTTGGTATTCTCGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCG
CAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTG
GTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:439
Protein Molecular Weight:46041
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 2-22
  • 25-45
  • 52-72
  • 106-126
  • 127-147
  • 152-172
  • 173-193
  • 226-246
  • 255-275
  • 291-311
  • 327-347
  • 349-369
  • 371-391
  • 418-438
Protein Sequence:
>Gnt-II system L-idonate transporter
MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQAVLHSIQNGIGSTLGG
LAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALVITGLVVGLAMFFEVGFVL
LLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGPTAIATIFEANLGTTLLYGFI
ITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEEMPSFWNSIFAAVIPVILMAIAA
VCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFTLGRRNGRTIEQIMDIIGDSIGAIA
MIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTLSPLLMCWTVAALLRIALGSATVAAIT
TAGVVLPIINVTHADPALMVLATGAGSVIASHVNDPGFWLFKGYFNLTVGETLRTWTVME
TLISIMGLLGVLAINAVLH
References
External Links:
ResourceLink
Uniprot ID:P39344
Uniprot Name:IDNT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674541
Ecogene ID:EG12539
Ecocyc:EG12539
ColiBase:b4265
Kegg Gene:b4265
EchoBASE ID:EB2428
CCDB:IDNT_ECOLI
BacMap:16132087
General Reference:
  • Bausch, C., Peekhaus, N., Utz, C., Blais, T., Murray, E., Lowary, T., Conway, T. (1998). "Sequence analysis of the GntII (subsidiary) system for gluconate metabolism reveals a novel pathway for L-idonic acid catabolism in Escherichia coli." J Bacteriol 180:3704-3710. Pubmed: 9658018
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Reed, J. L., Patel, T. R., Chen, K. H., Joyce, A. R., Applebee, M. K., Herring, C. D., Bui, O. T., Knight, E. M., Fong, S. S., Palsson, B. O. (2006). "Systems approach to refining genome annotation." Proc Natl Acad Sci U S A 103:17480-17484. Pubmed: 17088549