ECMDB: Thermosensitive gluconokinase (P39208)

Identification

Name:

Thermosensitive gluconokinase

Synonyms:

Gluconate kinase 1

Gene Name:

idnK

Enzyme Class:

2.7.1.12

Biological Properties

General Function:

Involved in shikimate kinase activity

Specific Function:

ATP + D-gluconate = ADP + 6-phospho-D- gluconate

Cellular Location:

Not Available

SMPDB Pathways:

ketogluconate metabolism PW002003

KEGG Pathways:

Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Pentose phosphate pathway ec00030

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid ↔ 1.06-Phosphogluconic acid + 1.0ADP + 1.0Hydrogen ion
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid ↔ 1.0ADP + 1.06-Phosphogluconic acid
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0gluconate 6-phosphate

1.0Gluconic acid + 1.0Adenosine triphosphate → 1.0ADP + 1.0Hydrogen ion + 1.0gluconate 6-phosphate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid ↔ 1.06-Phosphogluconic acid + 1.0ADP + 1.0Hydrogen ion
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid ↔ 1.0ADP + 1.06-Phosphogluconic acid
ReactionCard

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid → 1.0Hydrogen ion + 1.0ADP + 1.06-Phosphogluconic acid
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01316	6-Phosphogluconic acid	MetaboCard
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB00625	Gluconic acid	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard

GO Classification:

Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
nucleoside binding
purine nucleoside binding
shikimate kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
carbohydrate metabolic process
metabolic process
primary metabolic process

Gene Properties

Blattner:

b4268

Gene Orientation

Clockwise

Centisome Percentage:

96.83

Left Sequence End

4492646

Right Sequence End

4493209

Gene Sequence:

>564 bp
ATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAA
TTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCG
GCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAA
CTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAA
GCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAATATGCGATGATCGGCGACCCG
ACTGGCGCCCTGACCCGTAACTTCGACAACATGCGTGAAGATGAAGGTCTGGCTGACCGT
GCGACCTTCGTTGTTGACCCGCAGGGTATCATCCAGGCAATCGAAGTTACCGCTGAAGGC
ATTGGCCGTGACGCGTCTGACCTGCTGCGTAAAATCAAAGCAGCACAGTACGTAGCTTCT
CACCCAGGTGAAGTTTGCCCGGCTAAATGGAAAGAAGGTGAAGCAACTCTGGCTCCGTCT
CTGGACCTGGTTGGTAAAATCTAA

Protein Properties

Pfam Domain Function:

SKI (PF01202 )

Protein Residues:

187

Protein Molecular Weight:

21004

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Thermosensitive gluconokinase
MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNIDKMSQGIPLSDEDRLPW
LERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHFLWLDGDYETILARMQRRA
GHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTEQCRQAVLAIRQNRICAKEGS
ASDQRCE

References

External Links:

Resource	Link
Uniprot ID:	P39208
Uniprot Name:	IDNK_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062222
Ecogene ID:	EG12152
Ecocyc:	EG12152
ColiBase:	b4268
Kegg Gene:	b4268
EchoBASE ID:	EB2072
CCDB:	IDNK_ECOLI
BacMap:	16132090

General Reference:

Bausch, C., Peekhaus, N., Utz, C., Blais, T., Murray, E., Lowary, T., Conway, T. (1998). "Sequence analysis of the GntII (subsidiary) system for gluconate metabolism reveals a novel pathway for L-idonic acid catabolism in Escherichia coli." J Bacteriol 180:3704-3710. Pubmed: 9658018
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553