Cation efflux system protein cusA (P38054)

Identification
Name:Cation efflux system protein cusA
Synonyms:Not Available
Gene Name:cusA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Part of a cation efflux system that mediates resistance to copper and silver
Cellular Location:Cell inner membrane; Multi-pass membrane protein (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB00657CopperMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
cation transmembrane transporter activity
ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
transport
Gene Properties
Blattner:b0575
Gene OrientationClockwise
Centisome Percentage:12.89
Left Sequence End597937
Right Sequence End601080
Gene Sequence:
>3144 bp
ATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCG
TTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCG
GATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGTCAGGCACCACAAATC
GTTGAAAATCAGGTGACTTATCCGCTAACCACCACCATGTTGTCGGTGCCTGGCGCGAAG
ACTGTGCGCGGTTTCTCACAGTTTGGCGACTCTTATGTGTATGTCATTTTCGAAGATGGC
ACCGATCCGTACTGGGCGCGTTCGCGGGTGCTGGAGTACCTCAACCAGGTACAGGGTAAG
CTGCCTGCGGGAGTCAGCGCCGAGCTGGGGCCAGATGCCACGGGTGTTGGCTGGATCTAT
GAATATGCACTGGTGGATCGCAGCGGTAAGCACGATCTGGCCGATTTACGCTCATTACAG
GACTGGTTTCTCAAATATGAGCTGAAAACCATCCCTGACGTTGCGGAAGTGGCGTCGGTG
GGCGGTGTGGTGAAAGAGTATCAGGTGGTTATCGATCCCCAGCGCCTGGCGCAGTATGGC
ATCAGTCTCGCCGAAGTAAAAAGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTTCG
TCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTC
GACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGC
GATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGC
GAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTG
ATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAG
ATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGC
AAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTG
CGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTGGGGTTGTGTATTGCTTTTATTGTC
ATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTC
GGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATGCGCATAAACGGCTGGAAGAG
TGGCAGCACCAGCATCCTGACGCCACGCTGGATAATAAAACGCGCTGGCAGGTGATCACC
GATGCGTCTGTTGAAGTGGGGCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCG
TTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTC
ACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTG
ATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTT
TTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTG
CTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAA
TTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATT
TCCGCAGCAGAGGCGGCGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCT
GAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCG
CTGGAGATGGTAGAAACGACCATCCAGCTTAAGCCGCAGGAGCAGTGGCGGCCAGGCATG
ACGATGGACAAAATCATTGAGGAACTGGATAACACCGTGCGGCTGCCGGGGCTGGCGAAT
CTGTGGGTGCCGCCAATTCGTAACCGTATCGATATGCTCTCAACCGGCATTAAAAGCCCC
ATCGGCATTAAAGTTTCCGGCACTGTGCTGGCGGATATCGACGCGATGGCTGAGCAAATT
GAAGAAGTAGCGCGAACGGTGCCAGGCGTAGCTTCTGCGCTTGCCGAGCGGCTGGAAGGT
GGGCGCTATATCAACGTTGAGATTAACCGTGAAAAAGCCGCACGTTACGGTATGACGGTG
GCGGATGTGCAGTTGTTTGTGACTTCTGCGGTGGGCGGGGCGATGGTTGGCGAAACGGTG
GAAGGGATTGCCCGTTATCCAATTAATCTGCGTTATCCGCAAAGCTGGCGCGATAGTCCG
CAGGCACTGCGCCAGCTGCCGATCCTGACGCCGATGAAGCAGCAAATCACCCTGGCAGAC
GTGGCCGACATTAAAGTCTCTACCGGACCGTCGATGCTGAAAACCGAGAATGCGCGCCCG
ACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTG
CAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGG
CAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTG
ATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATT
ATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTT
CATCTTTCCGTGGCGACGGGCACTGGCTTTATCGCCCTCGCCGGGGTCGCCGCCGAATTT
GGCGTGGTGATGCTGATGTATTTACGTCACGCCATAGAGGCCGTGCCGTCGTTGAATAAT
CCACAAACATTCAGCGAGCAGAAGCTGGATGAGGCGTTATATCACGGCGCGGTCCTGCGC
GTGCGCCCGAAAGCGATGACGGTGGCGGTGATTATCGCCGGTCTGCTGCCGATTCTGTGG
GGAACGGGGGCTGGTTCAGAGGTGATGAGCCGGATTGCCGCGCCGATGATTGGCGGCATG
ATCACCGCACCTTTGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTG
CACCGACATCGGGTACGGAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:1047
Protein Molecular Weight:114706
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • 14-34
  • 338-358
  • 363-383
  • 391-411
  • 446-466
  • 485-505
  • 532-552
  • 871-891
  • 898-918
  • 928-948
  • 985-1005
  • 1012-1032
Protein Sequence:
>Cation efflux system protein cusA
MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSDVQVIIKTSYPGQAPQI
VENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTDPYWARSRVLEYLNQVQGK
LPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQDWFLKYELKTIPDVAEVASV
GGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAGGSSIELAEAEYMVRASGYLQTL
DDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIAELNGEGEVAGGVVILRSGKNAREV
IAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAIDNLSGKLLEEFIVVAVVCALFLWHV
RSALVAIISLPLGLCIAFIVMHFQGLNANIMSLGGIAIAVGAMVDAAIVMIENAHKRLEE
WQHQHPDATLDNKTRWQVITDASVEVGPALFISLLIITLSFIPIFTLEGQEGRLFGPLAF
TKTYAMAGAALLAIVVIPILMGYWIRGKIPPESSNPLNRFLIRVYHPLLLKVLHWPKTTL
LVAALSVLTVLWPLNKVGGEFLPQINEGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVP
EVARVFGKTGKAETATDSAPLEMVETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLAN
LWVPPIRNRIDMLSTGIKSPIGIKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEG
GRYINVEINREKAARYGMTVADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSP
QALRQLPILTPMKQQITLADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDL
QKAIAEKVQLKPGTSVAFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLI
ISSVPFALVGGIWLLWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNN
PQTFSEQKLDEALYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGM
ITAPLLSLFIIPAAYKLMWLHRHRVRK
References
External Links:
ResourceLink
Uniprot ID:P38054
Uniprot Name:CUSA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062205
Ecogene ID:EG12367
Ecocyc:EG12367
ColiBase:b0575
Kegg Gene:b0575
EchoBASE ID:EB2270
CCDB:CUSA_ECOLI
BacMap:16128558
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., Rudd, K. E., Koonin, E. V. (1994). "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Nucleic Acids Res 22:4756-4767. Pubmed: 7984428
  • Franke, S., Grass, G., Nies, D. H. (2001). "The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions." Microbiology 147:965-972. Pubmed: 11283292
  • Franke, S., Grass, G., Rensing, C., Nies, D. H. (2003). "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli." J Bacteriol 185:3804-3812. Pubmed: 12813074
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Munson, G. P., Lam, D. L., Outten, F. W., O'Halloran, T. V. (2000). "Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12." J Bacteriol 182:5864-5871. Pubmed: 11004187
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Outten, F. W., Huffman, D. L., Hale, J. A., O'Halloran, T. V. (2001). "The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli." J Biol Chem 276:30670-30677. Pubmed: 11399769
  • Pi, J., Wookey, P. J., Pittard, A. J. (1991). "Cloning and sequencing of the pheP gene, which encodes the phenylalanine-specific transport system of Escherichia coli." J Bacteriol 173:3622-3629. Pubmed: 1711024