Identification |
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Name: | Phosphoethanolamine transferase eptB |
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Synonyms: | Not Available |
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Gene Name: | eptB |
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Enzyme Class: | Not Available |
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Biological Properties |
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General Function: | Involved in catalytic activity |
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Specific Function: | Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases diacylglycerol |
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Cellular Location: | Cell inner membrane; Multi-pass membrane protein |
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SMPDB Pathways: | - Lipopolysaccharide biosynthesis PW000831
- lipopolysaccharide biosynthesis II PW001905
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KEGG Pathways: | - Lipopolysaccharide biosynthesis ec00540
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SMPDB Reactions: | |
1.0a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA | + | 1.0 | + | 1.0 | → | 1.0 | + | 1.0phosphoethanolamine-Kdo2-lipid A | + | 1.0 |
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1.0 | + | 1.0a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA | + | 1.0 | → | 1.0phosphoethanolamine-Kdo2-lipid A | + | 1.01,2-dihexadecanoyl-rac-glycerol | + | 1.0 |
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EcoCyc Reactions: | |
1.0 | + | 1.0α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IVA | ? | 1.0a 1,2-diacylglycerol | + | 1.0phosphatidylethanolamine-KDO2-lipidA |
| 1.0 PE(14:0/14:0) + 1.0α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IV A ? 1.0a 1,2-diacylglycerol + 1.0phosphatidylethanolamine-KDO 2-lipidA ReactionCard |
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Complex Reactions: | |
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Metabolites: | ECMDB ID | Name | View |
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ECMDB21103 | 1,2-Diacyl-sn-glycerol (didodecanoyl, n-C12:0) | MetaboCard | ECMDB21104 | 1,2-Diacyl-sn-glycerol (dihexadec-9-enoyl, n-C16:1) | MetaboCard | ECMDB21106 | 1,2-Diacyl-sn-glycerol (dioctadec-11-enoyl, n-C18:1) | MetaboCard | ECMDB24178 | a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA | MetaboCard | ECMDB21031 | KDO2-Lipid A | MetaboCard | ECMDB21417 | O-Phosphoethanolamine | MetaboCard | ECMDB21599 | PE(12:0/10:0) | MetaboCard | ECMDB08821 | PE(14:0/14:0) | MetaboCard | ECMDB08923 | PE(16:0/16:0) | MetaboCard | ECMDB21352 | Phosphoethanolamine KDO(2)-lipid (A) | MetaboCard |
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GO Classification: | Component |
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cell part | integral to membrane | intrinsic to membrane | membrane part | Function |
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catalytic activity | hydrolase activity | hydrolase activity, acting on ester bonds | sulfuric ester hydrolase activity | Process |
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metabolic process |
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Gene Properties |
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Blattner: | b3546 |
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Gene Orientation | Counterclockwise |
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Centisome Percentage: | 79.89 |
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Left Sequence End | 3706807 |
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Right Sequence End | 3708498 |
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Gene Sequence: | >1692 bp
ATGAGATACATCAAATCGATTACACAGCAGAAGCTGAGCTTTTTGCTTGCAATCTATATT
GGCCTTTTTATGAATGGCGCGGTTTTTTACCGCCGCTTCGGCAGCTATGCGCACGATTTT
ACCGTCTGGAAAGGCATTTCTGCTGTTGTTGAACTGGCCGCCACCGTACTGGTGACCTTC
TTTTTACTACGTCTTCTTTCGCTGTTTGGCCGCCGCAGCTGGCGTATTCTGGCATCGCTG
GTGGTGCTCTTTTCCGCAGGTGCCAGCTATTACATGACCTTCCTTAATGTGGTCATTGGT
TATGGCATCATCGCTTCCGTCATGACCACCGATATCGACCTGTCAAAAGAAGTTGTTGGT
CTGAACTTTATTCTCTGGTTAATCGCCGTTAGTGCATTGCCTCTTATCCTTATCTGGAAT
AACCGCTGTCGCTACACCTTGCTCCGACAACTGCGAACCCCAGGGCAGCGTATTCGCAGC
CTGGCGGTCGTCGTACTGGCGGGTATTATGGTTTGGGCACCGATTCGTTTGCTGGATATC
CAGCAGAAGAAAGTGGAGAGGGCGACCGGCGTTGATTTGCCGAGTTATGGCGGTGTCGTA
GCGAACTCTTATCTGCCATCAAACTGGCTTTCTGCGTTGGGGCTGTATGCCTGGGCGCGG
GTCGATGAATCTTCCGATAATAATTCATTGCTTAATCCGGCGAAGAAATTCACTTATCAG
GCACCGCAAAACGTTGATGACACTTATGTCGTGTTTATCATCGGTGAAACCACGCGTTGG
GACCATATGGGTATTTTCGGCTATGAGCGTAATACCACGCCGAAACTGGCCCAGGAGAAA
AATCTGGCGGCGTTCCGTGGTTACTCCTGTGATACCGCAACCAAACTCTCACTGCGTTGC
ATGTTTGTACGTCAGGGGGGCGCGGAAGATAATCCGCAGCGCACATTAAAAGAACAGAAC
ATTTTCGCGGTTCTGAAGCAGTTAGGATTCAGTTCTGACCTCTACGCTATGCAGAGCGAA
ATGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGCG
GAGCCACGTAATCGTGGCAAGCCGGTAGATGATATGTTGCTGGTAGACGAAATGCAGCAA
TCGCTAGGGCGCAACCCGGATGGTAAGCATCTGATCATTCTGCATACCAAAGGTTCGCAT
TTTAACTACACCCAGCGTTATCCGCGTAGCTTCGCGCAGTGGAAGCCGGAATGTATTGGT
GTTGATAGCGGCTGTACCAAAGCGCAGATGATCAACTCCTATGACAACTCGGTGACCTAT
GTGGATCACTTTATCTCCAGCGTGATTGATCAGGTTCGCGATAAGAAAGCGATTGTGTTC
TACGCAGCTGACCACGGTGAGTCAATTAATGAACGCGAGCACCTGCACGGCACGCCGCGT
GAACTGGCACCGCCGGAGCAGTTCCGCGTACCGATGATGGTCTGGATGTCAGATAAATAT
CTGGAAAATCCGGCCAATGCGCAGGCGTTTGCGCAGCTGAAAAAAGAAGCCGACATGAAA
GTGCCACGCCGTCACGTAGAGCTGTACGATACCATCATGGGTTGTCTTGGCTATACTTCA
CCGGATGGTGGAATTAACGAAAACAACAACTGGTGTCACATCCCGCAGGCAAAAGAGGCA
GCGGCTAACTAA |
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Protein Properties |
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Pfam Domain Function: | |
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Protein Residues: | 563 |
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Protein Molecular Weight: | 63804 |
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Protein Theoretical pI: | 9 |
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Signaling Regions: | |
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Transmembrane Regions: | - 10-30
- 49-69
- 80-100
- 118-138
- 160-180
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Protein Sequence: | >Phosphoethanolamine transferase eptB
MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWKGISAVVELAATVLVTF
FLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYGIIASVMTTDIDLSKEVVG
LNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRSLAVVVLAGIMVWAPIRLLDI
QQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAWARVDESSDNNSLLNPAKKFTYQ
APQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKLAQEKNLAAFRGYSCDTATKLSLRC
MFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDLYAMQSEMWFYSNTMADNIAYREQIGA
EPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLIILHTKGSHFNYTQRYPRSFAQWKPECIG
VDSGCTKAQMINSYDNSVTYVDHFISSVIDQVRDKKAIVFYAADHGESINEREHLHGTPR
ELAPPEQFRVPMMVWMSDKYLENPANAQAFAQLKKEADMKVPRRHVELYDTIMGCLGYTS
PDGGINENNNWCHIPQAKEAAAN |
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References |
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External Links: | |
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General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
- Reynolds, C. M., Kalb, S. R., Cotter, R. J., Raetz, C. R. (2005). "A phosphoethanolamine transferase specific for the outer 3-deoxy-D-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. Identification of the eptB gene and Ca2+ hypersensitivity of an eptB deletion mutant." J Biol Chem 280:21202-21211. Pubmed: 15795227
- Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
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