4'-phosphopantetheinyl transferase AcpT (P37623)

Identification
Name:4'-phosphopantetheinyl transferase AcpT
Synonyms:Not Available
Gene Name:acpT
Enzyme Class:Not Available
Biological Properties
General Function:Involved in magnesium ion binding
Specific Function:May be involved in an alternative pathway for phosphopantetheinyl transfer and holo-ACP synthesis in E.coli. The native apo-protein substrate is unknown. Is able to functionally replace AcpS in vivo but only when expressed at high levels
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Pantothenate and CoA biosynthesis ec00770
KEGG Reactions:
1.0Thumb+1.0Apo-[acyl-carrier-protein] ↔ 1.0Thumb+1.0Acyl-carrier protein+1.0Acyl-carrier protein
1.0Coenzyme A + 1.0Apo-[acyl-carrier-protein] ↔ 1.0Adenosine 3',5'-diphosphate + 1.0Acyl-carrier protein + 1.0Acyl-carrier protein
ReactionCard
Complex Reactions:
1.0apoprotein [acyl carrier protein]+1.0Thumb → 1.0acyl carrier protein+1.0Thumb+1.0Thumb
1.0apoprotein [acyl carrier protein] + 1.0Coenzyme A → 1.0acyl carrier protein + 1.0Hydrogen ion + 1.0Adenosine 3',5'-diphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00061Adenosine 3',5'-diphosphateMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
holo-[acyl-carrier-protein] synthase activity
ion binding
magnesium ion binding
metal ion binding
phosphotransferase activity, for other substituted phosphate groups
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
macromolecule biosynthetic process
metabolic process
Gene Properties
Blattner:b3475
Gene OrientationClockwise
Centisome Percentage:77.83
Left Sequence End3610992
Right Sequence End3611579
Gene Sequence:
>588 bp
ATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTAT
CAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTT
GAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAG
CTGGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGT
CGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGT
GAACAGATGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTG
GGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAA
GAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTGTGGTACACGGTATTTTTGCC
AGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTG
GTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:195
Protein Molecular Weight:21768
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>4'-phosphopantetheinyl transferase AcpT
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPA
FAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAV
HPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSSLSVSHCQLENLSLAICTPTP
FTLTADSVQWIDSVN
References
External Links:
ResourceLink
Uniprot ID:P37623
Uniprot Name:ACPT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674278
Ecogene ID:EG12221
Ecocyc:EG12221
ColiBase:b3475
Kegg Gene:b3475
EchoBASE ID:EB2135
CCDB:ACPT_ECOLI
BacMap:16131347
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Flugel, R. S., Hwangbo, Y., Lambalot, R. H., Cronan, J. E. Jr, Walsh, C. T. (2000). "Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli." J Biol Chem 275:959-968. Pubmed: 10625633
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lambalot, R. H., Gehring, A. M., Flugel, R. S., Zuber, P., LaCelle, M., Marahiel, M. A., Reid, R., Khosla, C., Walsh, C. T. (1996). "A new enzyme superfamily - the phosphopantetheinyl transferases." Chem Biol 3:923-936. Pubmed: 8939709
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620