Malate synthase G (P37330)

Identification
Name:Malate synthase G
Synonyms:
  • MSG
Gene Name:glcB
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Accounts for almost the entire malate-synthesizing activity in cells metabolizing glyoxylate
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Secondary Metabolites: Glyoxylate cycle PW000967
  • glycolate and glyoxylate degradation PW000827
  • glycolate and glyoxylate degradation II PW002021
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Glyoxylic acid + 1.0Water ↔ 1.0Coenzyme A + 1.0Hydrogen ion + 1.0L-Malic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Malic acid + 1.0Coenzyme A ↔ 1.0Acetyl-CoA + 1.0Water + 1.0Glyoxylic acid
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0L-Malic acid+1.0Thumb
1.0Glyoxylic acid + 1.0Water + 1.0Acetyl-CoA → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0L-Malic acid + 1.0L-Malic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Glyoxylic acid + 1.0Water ↔ 1.0Coenzyme A + 1.0Hydrogen ion + 1.0L-Malic acid
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Water + 1.0Glyoxylic acid → 1.0Hydrogen ion + 1.0L-Malic acid + 1.0Coenzyme A
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01206Acetyl-CoAMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB00119Glyoxylic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00156L-Malic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
malate synthase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
Process
carbohydrate metabolic process
cellular carbohydrate metabolic process
glyoxylate cycle
metabolic process
primary metabolic process
Gene Properties
Blattner:b2976
Gene OrientationCounterclockwise
Centisome Percentage:67.24
Left Sequence End3119656
Right Sequence End3121827
Gene Sequence:
>2172 bp
ATGAGTCAAACCATAACCCAGAGCCGTTTACGCATTGACGCCAATTTTAAACGTTTTGTG
GATGAAGAAGTTTTACCGGGAACAGGGCTGGACGCTGCGGCGTTCTGGCGCAATTTTGAT
GAGATCGTTCATGATCTGGCACCAGAAAATCGTCAGTTGCTGGCAGAACGCGATCGCATT
CAGGCAGCGCTTGATGAGTGGCATCGCAGCAATCCGGGGCCGGTAAAAGATAAAGCGGCC
TATAAATCTTTCCTGCGTGAACTGGGCTACCTGGTGCCGCAACCGGAGCGCGTGACGGTG
GAAACCACGGGCATTGACAGCGAAATCACCAGCCAGGCGGGGCCGCAGCTGGTGGTTCCG
GCAATGAACGCCCGCTACGCGCTGAACGCGGCGAACGCTCGCTGGGGCTCACTGTACGAT
GCGTTATACGGCAGCGACATCATCCCGCAGGAAGGGGCGATGGTCAGCGGCTACGATCCG
CAACGCGGTGAGCAGGTTATCGCCTGGGTTCGGCGTTTCCTCGATGAATCTCTACCGCTG
GAAAACGGCAGCTATCAGGATGTGGTGGCGTTTAAGGTGGTTGATAAACAATTACGCATC
CAGTTGAAAAATGGTAAAGAAACCACGTTACGTACTCCAGCACAGTTTGTCGGTTACCGT
GGCGATGCCGCTGCGCCGACCTGCATTTTGCTGAAAAATAACGGCCTGCATATTGAGCTG
CAAATCGATGCCAATGGGCGGATTGGCAAAGACGATCCGGCGCACATCAACGATGTTATC
GTCGAAGCTGCTATCAGTACCATTCTCGACTGCGAAGATTCGGTCGCGGCGGTTGATGCG
GAAGATAAAATCCTGCTGTACCGCAACCTGCTGGGCCTGATGCAGGGGACTCTGCAAGAG
AAAATGGAGAAAAACGGTCGGCAAATCGTGCGTAAACTGAATGACGATCGTCATTACACC
GCCGCCGATGGCTCTGAAATTTCTCTGCACGGACGCTCGCTGCTGTTTATCCGCAACGTG
GGTCATTTGATGACCATTCCTGTGATTTGGGACAGCGAAGGCAATGAAATCCCGGAAGGC
ATTCTTGATGGCGTCATGACTGGCGCGATTGCCCTCTATGATTTAAAAGTGCAGAAAAAC
TCGCGCACTGGCAGCGTCTATATTGTGAAACCGAAAATGCACGGTCCGCAGGAAGTGGCG
TTCGCCAACAAACTGTTTACCCGCATTGAGACAATGCTCGGTATGGCACCGAATACCCTG
AAAATGGGCATTATGGATGAAGAACGTCGGACCTCGCTGAACTTGCGTAGCTGTATCGCT
CAGGCGCGCAACCGCGTGGCGTTCATCAATACCGGTTTCCTCGACCGTACCGGCGATGAA
ATGCATTCGGTGATGGAAGCTGGCCCGATGCTGCGTAAAAATCAGATGAAATCGACGCCT
TGGATCAAAGCCTACGAGCGTAATAACGTGCTTTCCGGTCTGTTCTGTGGGCTGCGCGGT
AAAGCGCAAATTGGTAAAGGCATGTGGGCAATGCCGGACCTGATGGCAGACATGTACAGC
CAGAAGGGCGACCAACTGCGTGCCGGGGCAAACACAGCCTGGGTTCCGTCACCAACCGCT
GCTACGCTCCATGCGCTGCACTACCACCAAACCAACGTACAGAGCGTACAAGCCAACATT
GCCCAGACCGAGTTCAATGCTGAATTTGAACCGCTGCTGGACGATCTGCTGACTATTCCG
GTTGCTGAAAACGCTAACTGGTCGGCGCAAGAGATCCAACAAGAGCTGGATAACAACGTG
CAGGGGATTCTGGGGTACGTGGTGCGCTGGGTGGAGCAGGGGATTGGTTGTTCAAAAGTG
CCGGATATTCACAATGTGGCGTTGATGGAAGACCGCGCAACGCTGCGTATCTCCAGCCAG
CATATCGCCAACTGGTTACGTCACGGTATTCTGACCAAAGAACAGGTGCAGGCGTCGCTG
GAGAATATGGCGAAAGTGGTTGATCAGCAAAACGCTGGCGATCCGGCTTATCGTCCGATG
GCGGGGAATTTCGCTAACTCGTGTGCTTTTAAAGCTGCCAGCGATTTAATCTTCCTCGGC
GTGAAACAGCCAAACGGCTATACCGAACCGTTATTACACGCCTGGCGTTTACGCGAAAAA
GAAAGTCATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:723
Protein Molecular Weight:80488
Protein Theoretical pI:6
PDB File:1Y8B
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Malate synthase G
MSQTITQSRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVHDLAPENRQLLAERDRI
QAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVETTGIDSEITSQAGPQLVVP
AMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDPQRGEQVIAWVRRFLDESLPL
ENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVGYRGDAAAPTCILLKNNGLHIEL
QIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVAAVDAEDKILLYRNLLGLMQGTLQE
KMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSLLFIRNVGHLMTIPVIWDSEGNEIPEG
ILDGVMTGAIALYDLKVQKNSRTGSVYIVKPKMHGPQEVAFANKLFTRIETMLGMAPNTL
KMGIMDEERRTSLNLRSCIAQARNRVAFINTGFLDRTGDEMHSVMEAGPMLRKNQMKSTP
WIKAYERNNVLSGLFCGLRGKAQIGKGMWAMPDLMADMYSQKGDQLRAGANTAWVPSPTA
ATLHALHYHQTNVQSVQANIAQTEFNAEFEPLLDDLLTIPVAENANWSAQEIQQELDNNV
QGILGYVVRWVEQGIGCSKVPDIHNVALMEDRATLRISSQHIANWLRHGILTKEQVQASL
ENMAKVVDQQNAGDPAYRPMAGNFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREK
ESH
References
External Links:
ResourceLink
Uniprot ID:P37330
Uniprot Name:MASZ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675783
PDB ID:1Y8B
Ecogene ID:EG20080
Ecocyc:EG20080
ColiBase:b2976
Kegg Gene:b2976
EchoBASE ID:EB4141
CCDB:MASZ_ECOLI
BacMap:16130876
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Howard, B. R., Endrizzi, J. A., Remington, S. J. (2000). "Crystal structure of Escherichia coli malate synthase G complexed with magnesium and glyoxylate at 2.0 A resolution: mechanistic implications." Biochemistry 39:3156-3168. Pubmed: 10715138
  • Molina, I., Pellicer, M. T., Badia, J., Aguilar, J., Baldoma, L. (1994). "Molecular characterization of Escherichia coli malate synthase G. Differentiation with the malate synthase A isoenzyme." Eur J Biochem 224:541-548. Pubmed: 7925370