Probable Ni/Fe-hydrogenase 2 b-type cytochrome subunit (P37180)

Identification
Name:Probable Ni/Fe-hydrogenase 2 b-type cytochrome subunit
Synonyms:Not Available
Gene Name:hybB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in metal ion binding
Specific Function:Probable b-type cytochrome
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Ubiquinone-8 → 1.0Ubiquinol-8 + 2.0Hydrogen ion
ReactionCard
1.0Thumb+2.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
1.02-Demethylmenaquinone 8 + 2.0Hydrogen ion + 1.0Hydrogen (gas) → 1.02-Demethylmenaquinol 8 + 2.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb+1.0Menaquinone 8 → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Menaquinone 8 → 1.0Menaquinol 8 + 2.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB01362Hydrogen (gas)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b2995
Gene OrientationCounterclockwise
Centisome Percentage:67.70
Left Sequence End3141008
Right Sequence End3142186
Gene Sequence:
>1179 bp
ATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTG
GTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTG
AGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATT
GCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGA
AAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAAT
CGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCA
ATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTG
ATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTCCACCGTTG
GCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATA
TTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTG
CCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTA
TTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTG
TGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCA
GCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAG
CGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTC
TTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATT
GTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCT
ACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGA
GCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTT
GTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:392
Protein Molecular Weight:43602
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 12-32
  • 35-55
  • 59-79
  • 91-111
  • 134-154
  • 169-189
  • 208-228
  • 250-270
  • 282-302
  • 334-354
  • 356-376
Protein Sequence:
>Probable Ni/Fe-hydrogenase 2 b-type cytochrome subunit
MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSDLNGGFPWGVWIAFDLL
IGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLGGLSITIDVGRYWNLPYFY
IPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWKVSLQRLNKVMFFIIALGALL
PTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAFIMGFSIVIFEGSLVQAGLRGNG
PDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLSLAFAGDFYSVMFWIEVLLMLFPLV
VLRVAKLRNDSRMLFLSALSALLGCATWRLTYSLVAFNPGGGYAYFPTWEELLISIGFVA
IEICAYIVLIRLLPILPPLKQNDHNRHEASKA
References
External Links:
ResourceLink
Uniprot ID:P37180
Uniprot Name:HYBB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674315
Ecogene ID:EG11800
Ecocyc:EG11800
ColiBase:b2995
Kegg Gene:b2995
EchoBASE ID:EB1748
CCDB:HYBB_ECOLI
BacMap:16130895
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Menon, N. K., Chatelus, C. Y., Dervartanian, M., Wendt, J. C., Shanmugam, K. T., Peck, H. D. Jr, Przybyla, A. E. (1994). "Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2." J Bacteriol 176:4416-4423. Pubmed: 8021226