ECMDB: GDP-mannose pyrophosphatase nudK (P37128)

Identification

Name:

GDP-mannose pyrophosphatase nudK

Synonyms:

Not Available

Gene Name:

nudK

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Specific Function:

Catalyzes the hydrolysis of GDP-mannose. Can also use other substrates, catalyzing the hydrolysis of the pyrophosphate bond, releasing a nucleoside monophosphate and a phosphorylated moiety, depending on the substrate

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

EcoCyc Reactions:

1.0

→

1.0

2.0

1.0

1.0Guanosine diphosphate mannose + 1.0Water → 1.0Guanosine monophosphate + 2.0Hydrogen ion + 1.0D-Mannose 1-phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01436	D-Mannose 1-phosphate	MetaboCard
ECMDB01163	Guanosine diphosphate mannose	MetaboCard
ECMDB01397	Guanosine monophosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
ion binding
metal ion binding

Gene Properties

Blattner:

b2467

Gene Orientation

Counterclockwise

Centisome Percentage:

55.63

Left Sequence End

2580925

Right Sequence End

2581500

Gene Sequence:

>576 bp
GTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCAC
GGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGC
GAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGC
GGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCG
GACCGAGATGTCGATCTGGATGGTATCTATTATTGCCCGCATCATCCGCAGGGTAGTGTT
GAAGAGTTTCGCCAGGTCTGCGATTGCCGCAAACCACATCCGGGGATGCTTTTGTCAGCA
CGCGATTATTTGCATATTGATATGGCCGCTTCTTATATGGTGGGCGATAAATTAGAAGAT
ATGCAGGCAGCGGTTGCGGCGAACGTGGGAACAAAAGTGCTGGTGCGTACGGGTAAACCT
ATTACGCCTGAAGCAGAAAACGCGGCGGATTGGGTGTTAAATAGCCTGGCAGACCTGCCG
CAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGA

Protein Properties

Pfam Domain Function:

NUDIX (PF00293 )

Protein Residues:

191

Protein Molecular Weight:

21749

Protein Theoretical pI:

PDB File:

1VIU

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>GDP-mannose pyrophosphatase nudK
MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDRGNGATILLYNTKKKTV
VLIRQFRVATWVNGNESGQLIESCAGLLDNDEPEVCIRKEAIEETGYEVGEVRKLFELYM
SPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPFSQALEMIKTGEIRDGKTVLL
LNYLQTSHLMD

References

External Links:

Resource	Link
Uniprot ID:	P37128
Uniprot Name:	NUDK_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4902943
PDB ID:	1VIU
Ecogene ID:	EG12410
Ecocyc:	EG12410
ColiBase:	b2467
Kegg Gene:	b2467
EchoBASE ID:	EB2309
CCDB:	NUDK_ECOLI
BacMap:	16130392

General Reference:

Badger, J., Sauder, J. M., Adams, J. M., Antonysamy, S., Bain, K., Bergseid, M. G., Buchanan, S. G., Buchanan, M. D., Batiyenko, Y., Christopher, J. A., Emtage, S., Eroshkina, A., Feil, I., Furlong, E. B., Gajiwala, K. S., Gao, X., He, D., Hendle, J., Huber, A., Hoda, K., Kearins, P., Kissinger, C., Laubert, B., Lewis, H. A., Lin, J., Loomis, K., Lorimer, D., Louie, G., Maletic, M., Marsh, C. D., Miller, I., Molinari, J., Muller-Dieckmann, H. J., Newman, J. M., Noland, B. W., Pagarigan, B., Park, F., Peat, T. S., Post, K. W., Radojicic, S., Ramos, A., Romero, R., Rutter, M. E., Sanderson, W. E., Schwinn, K. D., Tresser, J., Winhoven, J., Wright, T. A., Wu, L., Xu, J., Harris, T. J. (2005). "Structural analysis of a set of proteins resulting from a bacterial genomics project." Proteins 60:787-796. Pubmed: 16021622
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Xu, W., Dunn, C. A., O'handley, S. F., Smith, D. L., Bessman, M. J. (2006). "Three new Nudix hydrolases from Escherichia coli." J Biol Chem 281:22794-22798. Pubmed: 16766526
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837