H(+)/Cl(-) exchange transporter ClcA (P37019)

Identification
Name:H(+)/Cl(-) exchange transporter ClcA
Synonyms:
  • ClC-ec1
Gene Name:clcA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in ion channel activity
Specific Function:Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response
Cellular Location:Cell inner membrane; Multi-pass membrane protein (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB04037ChlorideMetaboCard
ECMDB01362Hydrogen (gas)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
membrane
Function
anion channel activity
chloride channel activity
ion channel activity
ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
voltage-gated chloride channel activity
Process
anion transport
chloride transport
establishment of localization
inorganic anion transport
ion transport
transmembrane transport
transport
Gene Properties
Blattner:b0155
Gene OrientationClockwise
Centisome Percentage:3.77
Left Sequence End175107
Right Sequence End176528
Gene Sequence:
>1422 bp
ATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAA
CTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCA
GTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTG
CAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACC
GTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATAC
GCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGT
CCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGC
GGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGT
ATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACC
GGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATT
ATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATT
GGTGTCATTATGTCGACCATTATGTACCGGATTTTTAATCATGAAGTTGCGTTGATTGAC
GTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATT
ATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTG
CACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGT
CTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATT
CCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGG
GTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATG
CTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCG
CAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCA
TCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGATAACTACCAG
CTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGC
GGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAA
CTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATACTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:473
Protein Molecular Weight:50348
Protein Theoretical pI:10
PDB File:1KPK
Signaling Regions:
  • None
Transmembrane Regions:
  • 33-69
  • 77-100
  • 124-141
  • 148-166
  • 215-232
  • 253-281
  • 288-309
  • 330-349
  • 355-376
  • 422-438
Protein Sequence:
>H(+)/Cl(-) exchange transporter ClcA
MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGTLVGLAAVAFDKGVAWL
QNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAPEAGGSGIPEIEGALEDQR
PVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGRMVLDIFRLKGDEARHTLLAT
GAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAVFIGVIMSTIMYRIFNHEVALID
VGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGMQDLLHRVHGGNITKWVLMGGAIGG
LCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLVFIFVARVITTLLCFSSGAPGGIFAPM
LALGTVLGTAFGMVAVELFPQYHLEAGTFAIAGMGALLAASIRAPLTGIILVLEMTDNYQ
LILPMIITGLGATLLAQFTGGKPLYSAILARTLAKQEAEQLARSKAASASENT
References
External Links:
ResourceLink
Uniprot ID:P37019
Uniprot Name:CLCA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674361
PDB ID:1KPK
Ecogene ID:EG12331
Ecocyc:EG12331
ColiBase:b0155
Kegg Gene:b0155
CCDB:CLCA_ECOLI
BacMap:16128148
General Reference:
  • Accardi, A., Lobet, S., Williams, C., Miller, C., Dutzler, R. (2006). "Synergism between halide binding and proton transport in a CLC-type exchanger." J Mol Biol 362:691-699. Pubmed: 16949616
  • Accardi, A., Miller, C. (2004). "Secondary active transport mediated by a prokaryotic homologue of ClC Cl- channels." Nature 427:803-807. Pubmed: 14985752
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Dutzler, R., Campbell, E. B., Cadene, M., Chait, B. T., MacKinnon, R. (2002). "X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity." Nature 415:287-294. Pubmed: 11796999
  • Dutzler, R., Campbell, E. B., MacKinnon, R. (2003). "Gating the selectivity filter in ClC chloride channels." Science 300:108-112. Pubmed: 12649487
  • Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Iyer, R., Iverson, T. M., Accardi, A., Miller, C. (2002). "A biological role for prokaryotic ClC chloride channels." Nature 419:715-718. Pubmed: 12384697
  • Jayaram, H., Accardi, A., Wu, F., Williams, C., Miller, C. (2008). "Ion permeation through a Cl--selective channel designed from a CLC Cl-/H+ exchanger." Proc Natl Acad Sci U S A 105:11194-11199. Pubmed: 18678918
  • Lim, H. H., Miller, C. (2009). "Intracellular proton-transfer mutants in a CLC Cl-/H+ exchanger." J Gen Physiol 133:131-138. Pubmed: 19139174
  • Lobet, S., Dutzler, R. (2006). "Ion-binding properties of the ClC chloride selectivity filter." EMBO J 25:24-33. Pubmed: 16341087
  • Maduke, M., Pheasant, D. J., Miller, C. (1999). "High-level expression, functional reconstitution, and quaternary structure of a prokaryotic ClC-type chloride channel." J Gen Physiol 114:713-722. Pubmed: 10539975
  • Mindell, J. A., Maduke, M., Miller, C., Grigorieff, N. (2001). "Projection structure of a ClC-type chloride channel at 6.5 A resolution." Nature 409:219-223. Pubmed: 11196649
  • Nguitragool, W., Miller, C. (2006). "Uncoupling of a CLC Cl-/H+ exchange transporter by polyatomic anions." J Mol Biol 362:682-690. Pubmed: 16905147
  • Purdy, M. D., Wiener, M. C. (2000). "Expression, purification, and initial structural characterization of YadQ, a bacterial homolog of mammalian ClC chloride channel proteins." FEBS Lett 466:26-28. Pubmed: 10648805