PTS system trehalose-specific EIIBC component (P36672)

Identification
Name:PTS system trehalose-specific EIIBC component
Synonyms:
  • EIIBC-Tre
  • EII-Tre
  • Trehalose-specific phosphotransferase enzyme IIB component
  • PTS system trehalose-specific EIIB component
  • Trehalose permease IIC component
  • PTS system trehalose-specific EIIC component
Gene Name:treB
Enzyme Class:
Biological Properties
General Function:Involved in protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
Specific Function:The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in trehalose transport
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
KEGG Reactions:
1.0Thumb+1.0Protein N(pi)-phospho-L-histidine ↔ 1.0Thumb+1.0Protein histidine
1.0Trehalose + 1.0Protein N(pi)-phospho-L-histidine ↔ 1.0Trehalose 6-phosphate + 1.0Protein histidine
ReactionCard
1.0Protein N(pi)-phospho-L-histidine+1.0Sugar ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0HPr - phosphorylated → 1.0Thumb+1.0HPr
1.0α,α-trehalose + 1.0HPr - phosphorylated → 1.0Trehalose 6-phosphate + 1.0HPr
ReactionCard
1.0Thumb+1.0HPr - phosphorylated → 1.0Thumb+1.0HPr
1.0α,α-trehalose + 1.0HPr - phosphorylated → 1.0alpha,alpha-Trehalose 6-phosphate + 1.0HPr
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Trehalose → 1.0Pyruvic acid + 1.0Trehalose 6-phosphate
ReactionCard
Complex Reactions:
1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine+1.0Thumb → 1.0protein EIIB+1.0sugar phosphate
1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine + 1.0Sucrose → 1.0protein EIIB + 1.0sugar phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04010alpha,alpha-Trehalose 6-phosphateMetaboCard
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB00258SucroseMetaboCard
ECMDB00975TrehaloseMetaboCard
ECMDB01124Trehalose 6-phosphateMetaboCard
ECMDB24256α,α-trehaloseMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
alpha-glucoside transmembrane transporter activity
carbohydrate transmembrane transporter activity
cation transmembrane transporter activity
cation:sugar symporter activity
glucoside transmembrane transporter activity
ion transmembrane transporter activity
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
trehalose transmembrane transporter activity
Process
alpha-glucoside transport
carbohydrate transport
establishment of localization
glucoside transport
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport
trehalose transport
Gene Properties
Blattner:b4240
Gene OrientationCounterclockwise
Centisome Percentage:96.19
Left Sequence End4462782
Right Sequence End4464203
Gene Sequence:
>1422 bp
ATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAA
CTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCA
GTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTG
CAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACC
GTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATAC
GCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGT
CCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGC
GGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGT
ATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGCTGCTGGCAACC
GGTGCTGCTGCGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATT
ATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATT
GGTGTCATTATGTCGACCATTATGTACCGGATTTTTAATCATGAAGTTGCGTTGATTGAC
GTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATT
ATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTG
CACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGT
CTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATT
CCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGG
GTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATG
CTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCG
CAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCA
TCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGATAACTACCAG
CTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGC
GGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAA
CTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAATACTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:473
Protein Molecular Weight:51080
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 111-131
  • 159-179
  • 188-208
  • 226-246
  • 259-279
  • 301-321
  • 341-361
  • 371-391
  • 399-419
  • 441-461
Protein Sequence:
>PTS system trehalose-specific EIIBC component
MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANARPKEIEQLPMVKGCFTN
AGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARHNMKWHEQLISHFAVIFFPLLP
ALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFLWLIGEAIFFYLPVGICWSAV
KKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDFGMFSIAKVGYQAQVIPALLAGL
ALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHALIGPFGRMIGDGVAFAVRHLMTGS
FAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQSMGGTPVWPLIALSNIAQGSAVIGII
ISSRKHNEREISVPAAISAWLGVTEPAMYGINLKYRFPMLCAMIGSGLAGLLCGLNGVMA
NGIGVGGLPGILSIQPSYWQVFALAMAIAIIIPIVLTSFIYQRKYRLGTLDIV
References
External Links:
ResourceLink
Uniprot ID:P36672
Uniprot Name:PTTBC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674361
Ecogene ID:EG12127
Ecocyc:EG12127
ColiBase:b4240
Kegg Gene:b4240
EchoBASE ID:EB2048
CCDB:PTTBC_ECOLI
BacMap:16132062
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Klein, W., Horlacher, R., Boos, W. (1995). "Molecular analysis of treB encoding the Escherichia coli enzyme II specific for trehalose." J Bacteriol 177:4043-4052. Pubmed: 7608078