Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (P36562)

Identification
Name:Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Synonyms:
  • NN:DBI PRT
  • N(1)-alpha-phosphoribosyltransferase
Gene Name:cobT
Enzyme Class:
Biological Properties
General Function:Involved in nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity
Specific Function:Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6- dimethylbenzimidazole (DMB)
Cellular Location:Not Available
SMPDB Pathways:
  • adenosylcobalamin salvage from cobinamide PW001884
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Dimethylbenzimidazole + 1.0Nicotinamide ribotide ↔ 1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole + 1.0Hydrogen ion + 1.0Nicotinic acid
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0nicotinate beta-D-ribonucleotide+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Dimethylbenzimidazole + 1.0nicotinate beta-D-ribonucleotide + 1.0Nicotinamide ribotide → 1.0Hydrogen ion + 1.0Nicotinic acid + 1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Dimethylbenzimidazole + 1.0Nicotinamide ribotide ↔ 1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole + 1.0Hydrogen ion + 1.0Nicotinic acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Nicotinamide ribotide + 1.0Dimethylbenzimidazole → 1.0Nicotinic acid + 1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB03701DimethylbenzimidazoleMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB03882N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazoleMetaboCard
ECMDB00229Nicotinamide ribotideMetaboCard
ECMDB01488Nicotinic acidMetaboCard
GO Classification:
Function
catalytic activity
nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
Process
cellular nitrogen compound metabolic process
cobalamin biosynthetic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside biosynthetic process
nucleoside metabolic process
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process
Gene Properties
Blattner:b1991
Gene OrientationCounterclockwise
Centisome Percentage:44.43
Left Sequence End2061412
Right Sequence End2062491
Gene Sequence:
>1080 bp
ATGCAAATACTTGCCGATTTACTGAATACGATCCCAGCCATCGATTCTACCGCTATGTCG
CGTGCACAACGGCATATTGACGGGTTACTCAAACCTGTTGGTAGCCTGGGAAAGCTGGAG
GTGCTTGCCATACAACTGGCAGGAATGCCGGGGTTGAATGGCATACCGCATGTGGGCAAA
AAAGCGGTACTGGTTATGTGTGCCGATCACGGCGTCTGGGAGGAAGGGGTCGCTATTTCT
CCAAAAGAAGTGACAGCCATACAGGCTGAAAATATGACCCGTGGAACAACCGGCGTGTGT
GTGCTGGCAGAACAAGCGGGCGCTAACGTCCACGTAATTGATGTTGGTATTGATACTGCT
GAGCCTATCCCCGGGCTTATCAACATGCGTGTCGCACGAGGTAGCGGCAATATTGCTTCA
GCTCCGGCAATGAGTCGCCGTCAGGCTGAAAAGTTGCTTTTGGACGTCATATGTTATACG
CAGGAGCTGGCAAAAAACGGTGTCACGCTGTTTGGTGTAGGTGAACTGGGGATGGCAAAC
ACGACACCGGCAGCAGCAATAGTCAGCACAATCACTGGCCGGGATCCTGAAGAAGTGGTT
GGGATTGGCGCAAACCTGCCGACAGATAAACTGGCTAATAAAATTGATGTTGTGCGTCGG
GCGATTACGTTGAATCAACCAAATCCTCAGGATGGTGTTGATGTCCTGGCAAAAGTGGGT
GGATTTGATTTGGTCGGAATAGCTGGAGTGATGTTAGGTGCTGCTTCCTGCGGTTTACCC
GTGTTGCTGGATGGATTTCTTTCTTATGCTGCTGCGCTCGCAGCCTGCCAGATGTCTCCT
GCGATCAAACCGTATCTCATTCCTTCTCACCTGTCGGCAGAAAAAGGCGCGCGTATAGCG
CTCTCGCATTTGGGGCTGGAGCCTTATCTCAATATGGAGATGCGTTTAGGTGAGGGGAGT
GGCGCAGCTCTGGCGATGCCCATCATCGAAGCTGCCTGTGCGATATACAACAACATGGGC
GAACTTGCTGCCAGTAATATTGTTCTACCGGGGAATACGACTTCTGATTTGAACAGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:359
Protein Molecular Weight:36987
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
MQILADLLNTIPAIDSTAMSRAQRHIDGLLKPVGSLGKLEVLAIQLAGMPGLNGIPHVGK
KAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVLAEQAGANVHVIDVGIDTA
EPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYTQELAKNGVTLFGVGELGMAN
TTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVVRRAITLNQPNPQDGVDVLAKVG
GFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAACQMSPAIKPYLIPSHLSAEKGARIA
LSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACAIYNNMGELAASNIVLPGNTTSDLNS
References
External Links:
ResourceLink
Uniprot ID:P36562
Uniprot Name:COBT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736652
Ecogene ID:EG12151
Ecocyc:EG12151
ColiBase:b1991
Kegg Gene:b1991
EchoBASE ID:EB2071
CCDB:COBT_ECOLI
BacMap:16129932
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Lawrence, J. G., Roth, J. R. (1995). "The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli." J Bacteriol 177:6371-6380. Pubmed: 7592411