ECMDB: Probable N-acetylmuramoyl-L-alanine amidase AmiA (P36548)

Identification

Name:

Probable N-acetylmuramoyl-L-alanine amidase AmiA

Synonyms:

Not Available

Gene Name:

amiA

Enzyme Class:

3.5.1.28

Biological Properties

General Function:

Involved in N-acetylmuramoyl-L-alanine amidase activity

Specific Function:

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides

Cellular Location:

Secreted (Potential)

SMPDB Pathways:

Not Available

KEGG Pathways:

Cationic antimicrobial peptide (CAMP) resistance eco01503

KEGG Reactions:

1.0

↔

1.0

1.0N-Acetylmuramoyl-Ala + 1.0Water ↔ 1.0N-Acetyl-D-muramoate + 1.0L-Alanine
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + 1.0Water → 1.0L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard

1.0

→

1.0

1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + 1.0Water → 1.0L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00161	L-Alanine	MetaboCard
ECMDB21240	L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate	MetaboCard
ECMDB21239	L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine	MetaboCard
ECMDB21248	N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid	MetaboCard
ECMDB21249	N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide	MetaboCard
ECMDB21250	N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide	MetaboCard
ECMDB20176	N-Acetyl-D-muramoate	MetaboCard
ECMDB20178	N-Acetylmuramoyl-Ala	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
N-acetylmuramoyl-L-alanine amidase activity
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan catabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process

Gene Properties

Blattner:

b2435

Gene Orientation

Clockwise

Centisome Percentage:

54.97

Left Sequence End

2550374

Right Sequence End

2551243

Gene Sequence:

>870 bp
ATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAG
GGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACC
CCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCC
GTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGAC
TCCATTCTGGAAGCCATCGACGCAGGCATCAAACTGATTATCACCATCACTGAAGGCATC
CCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATC
GGCCCGAACTGCCCAGGCGTTATCACTCCGGGTGAATGCAAAATCGGTATCCAGCCTGGT
CACATTCACAAACCGGGTAAAGTGGGTATCGTTTCCCGTTCCGGTACACTGACCTATGAA
GCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGT
GACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAG
ACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGCTGAAGAAGAAGCAGCTGCG
TACATCAAAGAGCACGTTACCAAGCCAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCG
AAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGAT
GAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATC
GGTGAAGCACTGAAAACTGTTCTGAAATAA

Protein Properties

Pfam Domain Function:

Amidase_3 (PF01520 )

Protein Residues:

289

Protein Molecular Weight:

31412

Protein Theoretical pI:

Signaling Regions:

1-34

Transmembrane Regions:

None

Protein Sequence:

>Probable N-acetylmuramoyl-L-alanine amidase AmiA
MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNGHSKPKAKKSGGKRVVV
LDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEI
AHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
KDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR

References

External Links:

Resource	Link
Uniprot ID:	P36548
Uniprot Name:	AMIA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1651324
Ecogene ID:	EG11823
Ecocyc:	EG11823
ColiBase:	b2435
Kegg Gene:	b2435
EchoBASE ID:	EB1770
CCDB:	AMIA_ECOLI
BacMap:	16130360

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Tanaka, K., Muramatsu, S., Yamada, H., Mizuno, T. (1991). "Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance." Mol Gen Genet 226:367-376. Pubmed: 1903834
Troup, B., Jahn, M., Hungerer, C., Jahn, D. (1994). "Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant." J Bacteriol 176:673-680. Pubmed: 8300522
Tullman-Ercek, D., DeLisa, M. P., Kawarasaki, Y., Iranpour, P., Ribnicky, B., Palmer, T., Georgiou, G. (2007). "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." J Biol Chem 282:8309-8316. Pubmed: 17218314
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837