Probable N-acetylmuramoyl-L-alanine amidase AmiA (P36548)

Identification
Name:Probable N-acetylmuramoyl-L-alanine amidase AmiA
Synonyms:Not Available
Gene Name:amiA
Enzyme Class:
Biological Properties
General Function:Involved in N-acetylmuramoyl-L-alanine amidase activity
Specific Function:Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Cellular Location:Secreted (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:
  • Cationic antimicrobial peptide (CAMP) resistance eco01503
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0N-Acetylmuramoyl-Ala + 1.0Water ↔ 1.0N-Acetyl-D-muramoate + 1.0L-Alanine
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + 1.0Water → 1.0L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + 1.0Water → 1.0L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00161L-AlanineMetaboCard
ECMDB21240L-alanine-D-glutamate-meso-2,6-diaminoheptanedioateMetaboCard
ECMDB21239L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanineMetaboCard
ECMDB21248N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acidMetaboCard
ECMDB21249N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
ECMDB21250N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
ECMDB20176N-Acetyl-D-muramoateMetaboCard
ECMDB20178N-Acetylmuramoyl-AlaMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
N-acetylmuramoyl-L-alanine amidase activity
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan catabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b2435
Gene OrientationClockwise
Centisome Percentage:54.97
Left Sequence End2550374
Right Sequence End2551243
Gene Sequence:
>870 bp
ATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAG
GGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACC
CCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCC
GTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGAC
TCCATTCTGGAAGCCATCGACGCAGGCATCAAACTGATTATCACCATCACTGAAGGCATC
CCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATC
GGCCCGAACTGCCCAGGCGTTATCACTCCGGGTGAATGCAAAATCGGTATCCAGCCTGGT
CACATTCACAAACCGGGTAAAGTGGGTATCGTTTCCCGTTCCGGTACACTGACCTATGAA
GCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGT
GACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAG
ACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGCTGAAGAAGAAGCAGCTGCG
TACATCAAAGAGCACGTTACCAAGCCAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCG
AAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGAT
GAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATC
GGTGAAGCACTGAAAACTGTTCTGAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:289
Protein Molecular Weight:31412
Protein Theoretical pI:11
Signaling Regions:
  • 1-34
Transmembrane Regions:
  • None
Protein Sequence:
>Probable N-acetylmuramoyl-L-alanine amidase AmiA
MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNGHSKPKAKKSGGKRVVV
LDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEI
AHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
KDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR
References
External Links:
ResourceLink
Uniprot ID:P36548
Uniprot Name:AMIA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651324
Ecogene ID:EG11823
Ecocyc:EG11823
ColiBase:b2435
Kegg Gene:b2435
EchoBASE ID:EB1770
CCDB:AMIA_ECOLI
BacMap:16130360
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Tanaka, K., Muramatsu, S., Yamada, H., Mizuno, T. (1991). "Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance." Mol Gen Genet 226:367-376. Pubmed: 1903834
  • Troup, B., Jahn, M., Hungerer, C., Jahn, D. (1994). "Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant." J Bacteriol 176:673-680. Pubmed: 8300522
  • Tullman-Ercek, D., DeLisa, M. P., Kawarasaki, Y., Iranpour, P., Ribnicky, B., Palmer, T., Georgiou, G. (2007). "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." J Biol Chem 282:8309-8316. Pubmed: 17218314
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837