S-formylglutathione hydrolase yeiG (P33018)

Identification
Name:S-formylglutathione hydrolase yeiG
Synonyms:
  • FGH
Gene Name:yeiG
Enzyme Class:
Biological Properties
General Function:Involved in carboxylesterase activity
Specific Function:Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate. Shows also esterase activity against alpha-naphthyl acetate, lactoylglutathione, palmitoyl-CoA and several pNP-esters of short chain fatty acids
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
  • Methane metabolism ec00680
  • Microbial metabolism in diverse environments ec01120
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0S-Formylglutathione + 1.0Water ↔ 1.0Formic acid + 1.0Glutathione + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0S-Formylglutathione + 1.0Water ↔ 1.0Formic acid + 1.0Glutathione
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0S-Formylglutathione + 1.0Water ↔ 1.0Formic acid + 1.0Glutathione + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00142Formic acidMetaboCard
ECMDB00125GlutathioneMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01550S-FormylglutathioneMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cytoplasmic membrane-bounded vesicle
cytoplasmic vesicle
organelle
vesicle
Function
carboxylesterase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
S-formylglutathione hydrolase activity
thiolester hydrolase activity
Gene Properties
Blattner:b2154
Gene OrientationClockwise
Centisome Percentage:48.32
Left Sequence End2241932
Right Sequence End2242768
Gene Sequence:
>837 bp
ATGGAAATGCTCGAAGAGCACCGCTGTTTTGAAGGCTGGCAGCAACGCTGGCGACACGAC
TCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCAC
ACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACC
ACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTACTGGTGATGCCAGAC
ACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCA
GGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTAT
CTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCC
ATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGT
AAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGA
ATCAAAGCGTTTAGCAGCTATTTAGGTGAGGACAAAAATGCATGGCTGGAATGGGACAGT
TGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATCGATCAGGGC
GATAATGATCAGTTTCTTGCCGACCAGTTGCAACCTGCGGTACTGGCAGAAGCCGCGCGC
CAGAAAGCGTGGCCGATGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTC
ATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:278
Protein Molecular Weight:31259
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>S-formylglutathione hydrolase yeiG
MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPPVLYWLSGLTCNDENFT
TKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGFYLNATQPPWATHYRMYDY
LRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPGKYTSVSAFAPIVNPCSVPWG
IKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLIDQGDNDQFLADQLQPAVLAEAAR
QKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYLLK
References
External Links:
ResourceLink
Uniprot ID:P33018
Uniprot Name:SFGH2_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675268
Ecogene ID:EG12026
Ecocyc:EG12026
ColiBase:b2154
Kegg Gene:b2154
EchoBASE ID:EB1961
CCDB:SFGH2_ECOLI
BacMap:16130092
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gonzalez, C. F., Proudfoot, M., Brown, G., Korniyenko, Y., Mori, H., Savchenko, A. V., Yakunin, A. F. (2006). "Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG." J Biol Chem 281:14514-14522. Pubmed: 16567800
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kuznetsova, E., Proudfoot, M., Sanders, S. A., Reinking, J., Savchenko, A., Arrowsmith, C. H., Edwards, A. M., Yakunin, A. F. (2005). "Enzyme genomics: Application of general enzymatic screens to discover new enzymes." FEMS Microbiol Rev 29:263-279. Pubmed: 15808744