ECMDB

Identification

Name:

Protein nrfD

Synonyms:

Not Available

Gene Name:

nrfD

Enzyme Class:

Not Available

Biological Properties

General Function:

Inorganic ion transport and metabolism

Specific Function:

Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Complex Reactions:

3.0

2.0

1.0

→

3.0

2.0

1.0

3.0Ubiquinol-8 + 2.0Hydrogen ion + 1.0Nitrite → 3.0Ubiquinone-8 + 2.0Water + 1.0Ammonium
ReactionCard

3.0

2.0

1.0

→

3.0Menaquinone 8

2.0

1.0

3.0Menaquinol 8 + 2.0Hydrogen ion + 1.0Nitrite → 3.0Menaquinone 8 + 2.0Water + 1.0Ammonium
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21186	Ammonium	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB21245	Menaquinol 8	MetaboCard
ECMDB02786	Nitrite	MetaboCard
ECMDB01060	Ubiquinol-8	MetaboCard
ECMDB21296	Ubiquinone-8	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Not Available

Gene Properties

Blattner:

b4073

Gene Orientation

Clockwise

Centisome Percentage:

92.43

Left Sequence End

4288499

Right Sequence End

4289455

Gene Sequence:

>957 bp
ATGACGCAGACTTCCGCATTTCATTTTGAATCGCTGGTGTGGGACTGGCCGATTGCCATC
TACCTGTTTTTGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGC
TTCTACCCGCAGGCGGGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGG
CCGGGCGCGGTGATCCTCGGTCTGTTGATCCTCGTCTTCCACCTGACAAGACCGTGGACC
TTCTGGAAGCTGATGTTCCACTACAGTTTTACCTCGGTGATGTCGATGGGGGTGATGCTG
TTTCAGCTCTACATGGTGGTGCTGGTGCTGTGGCTGGCGAAAATCTTTGAACATGATTTG
CTTGCCCTGCAACAACGCTGGTTGCCGAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTG
CTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGG
GCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATC
CTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCC
ATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG
GAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCG
CTGGGCGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACG
TGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCG
TGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTC
GGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAA

Protein Properties

Pfam Domain Function:

NrfD (PF03916 )

Protein Residues:

318

Protein Molecular Weight:

35042

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

18-38
57-73
90-112
150-170
180-199
222-242
258-278
288-310

Protein Sequence:

>Protein nrfD
MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQAGGADSTLLRTTLIVG
PGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEHDL
LALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLGAYTGFLLSALKSYPFLNNPI
LPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVHRMEIPVVWGEIFLLVAFFVGLA
LGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPMLLKPWVNRSSGIPAVLAACGASLV
GVLMLRFFILYAGQLTVA

References

External Links:

Resource	Link
Uniprot ID:	P32709
Uniprot Name:	NRFD_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85676825
Ecogene ID:	EG11947
Ecocyc:	EG11947
ColiBase:	b4073
Kegg Gene:	b4073
EchoBASE ID:	EB1890
CCDB:	NRFD_ECOLI
BacMap:	16131899

General Reference:

Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Hussain, H., Grove, J., Griffiths, L., Busby, S., Cole, J. (1994). "A seven-gene operon essential for formate-dependent nitrite reduction to ammonia by enteric bacteria." Mol Microbiol 12:153-163. Pubmed: 8057835

Protein nrfD (P32709)