Identification
Name:Protein nrfD
Synonyms:Not Available
Gene Name:nrfD
Enzyme Class:Not Available
Biological Properties
General Function:Inorganic ion transport and metabolism
Specific Function:Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
3.0Thumb+2.0Thumb+1.0Thumb3.0Thumb+2.0Thumb+1.0Thumb
3.0Thumb+2.0Thumb+1.0Thumb3.0Menaquinone 8+2.0Thumb+1.0Thumb
3.0Menaquinol 8 + 2.0Hydrogen ion + 1.0Nitrite → 3.0Menaquinone 8 + 2.0Water + 1.0Ammonium
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21186AmmoniumMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB02786NitriteMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
ECMDB00494WaterMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b4073
Gene OrientationClockwise
Centisome Percentage:92.43
Left Sequence End4288499
Right Sequence End4289455
Gene Sequence:
>957 bp
ATGACGCAGACTTCCGCATTTCATTTTGAATCGCTGGTGTGGGACTGGCCGATTGCCATC
TACCTGTTTTTGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGC
TTCTACCCGCAGGCGGGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGG
CCGGGCGCGGTGATCCTCGGTCTGTTGATCCTCGTCTTCCACCTGACAAGACCGTGGACC
TTCTGGAAGCTGATGTTCCACTACAGTTTTACCTCGGTGATGTCGATGGGGGTGATGCTG
TTTCAGCTCTACATGGTGGTGCTGGTGCTGTGGCTGGCGAAAATCTTTGAACATGATTTG
CTTGCCCTGCAACAACGCTGGTTGCCGAAGCTGGGGATCGTGCAAAAGGTTCTGAGCCTG
CTGACGCCCGTTCATCGCGGACTGGAAACATTGATGCTGGTGCTGGCGGTGTTGTTGGGG
GCTTATACCGGCTTTCTGCTGTCGGCGCTGAAATCGTATCCGTTCCTCAATAACCCGATC
CTGCCGGTGCTGTTCCTCTTCTCCGGCATCTCGTCCGGTGCGGCGGTGGCGCTGATCGCC
ATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATG
GAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCG
CTGGGCGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACG
TGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCG
TGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTC
GGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:318
Protein Molecular Weight:35042
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 18-38
  • 57-73
  • 90-112
  • 150-170
  • 180-199
  • 222-242
  • 258-278
  • 288-310
Protein Sequence:
>Protein nrfD
MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQAGGADSTLLRTTLIVG
PGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEHDL
LALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLGAYTGFLLSALKSYPFLNNPI
LPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVHRMEIPVVWGEIFLLVAFFVGLA
LGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPMLLKPWVNRSSGIPAVLAACGASLV
GVLMLRFFILYAGQLTVA
References
External Links:
ResourceLink
Uniprot ID:P32709
Uniprot Name:NRFD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676825
Ecogene ID:EG11947
Ecocyc:EG11947
ColiBase:b4073
Kegg Gene:b4073
EchoBASE ID:EB1890
CCDB:NRFD_ECOLI
BacMap:16131899
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hussain, H., Grove, J., Griffiths, L., Busby, S., Cole, J. (1994). "A seven-gene operon essential for formate-dependent nitrite reduction to ammonia by enteric bacteria." Mol Microbiol 12:153-163. Pubmed: 8057835