Identification
Name:Multiphosphoryl transfer protein 2
Synonyms:
  • MTP 2
  • Phosphoenolpyruvate-protein phosphotransferase
  • Enzyme I-Ani
  • Phosphotransferase system enzyme I
  • Phosphocarrier protein HPr
  • Protein H
  • Fructose-like phosphotransferase enzyme IIA component
  • PTS system fructose-like EIIA component
Gene Name:ptsA
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity, transferring phosphorus-containing groups
Specific Function:The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:
  • Phosphotransferase system (PTS) ec02060
KEGG Reactions:
1.0Thumb+1.0Protein histidine1.0Thumb+1.0Protein N(pi)-phospho-L-histidine
1.0Phosphoenolpyruvic acid + 1.0Protein histidine ↔ 1.0Pyruvic acid + 1.0Protein N(pi)-phospho-L-histidine
ReactionCard
Complex Reactions:
1.0Protein EIIA N(pi)-phospho-L-histidine+1.0protein EIIB1.0protein EIIA+1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
1.0Protein EIIA N(pi)-phospho-L-histidine + 1.0protein EIIB → 1.0protein EIIA + 1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
phosphoenolpyruvate-protein phosphotransferase activity
phosphotransferase activity, nitrogenous group as acceptor
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
cellular metabolic process
establishment of localization
metabolic process
phosphate metabolic process
phosphoenolpyruvate-dependent sugar phosphotransferase system
phosphorus metabolic process
phosphorylation
transport
Gene Properties
Blattner:b3947
Gene OrientationCounterclockwise
Centisome Percentage:89.18
Left Sequence End4137743
Right Sequence End4140244
Gene Sequence:
>2502 bp
ATGGCCCTGATTGTGGAATTTATTTGTGAGCTACCTAACGGCGTACATGCGCGTCCGGCA
AGCCACGTTGAAACGCTGTGTAATACTTTTTCATCACAAATTGAGTGGCATAACCTGCGC
ACTGACCGCAAGGGCAACGCCAAAAGCGCCCTTGCGCTGATTGGCACCGATACGCTGGCG
GGCGATAACTGCCAGTTACTGATTTCCGGGGCCGACGAACAGGAAGCGCACCAGCGTTTA
AGCCAATGGCTGCGCGATGAATTCCCCCACTGCGATGCGCCGCTGGCGGAAGTTAAATCT
GACGAACTGGAACCACTGCCGGTTTCACTGACCAATCTGAATCCGCAAATTATCCGCGCC
CGCACCGTGTGCAGCGGTAGTGCAGGCGGCATTCTGACGCCGATCTCTTCTTTAGATCTC
AATGCGCTGGGTAATCTTCCCGCAGCCAAAGGCGTTGACGCCGAGCAATCCGCACTGGAA
AACGGCCTGACGCTGGTACTGAAAAACATTGAGTTTCGTCTGCTGGATAGCGACGGTGCT
ACCAGCGCGATTCTGGAAGCTCACCGATCCCTGGCTGGCGATACTTCCCTGCGCGAACAT
TTACTGGCAGGCGTCAGCGCCGGATTAAGCTGCGCCGAAGCAATTGTTGCCAGCGCGAAT
CACTTTTGCGAAGAGTTTTCCCGTTCCAGCAGCAGCTACCTGCAAGAACGTGCCCTGGAC
GTACGCGACGTCTGCTTCCAGTTACTCCAGCAAATCTACGGTGAGCAACGCTTCCCGGCA
CCGGGCAAACTGACGCAGCCCGCCATTTGTATGGCTGATGAACTGACCCCCAGCCAGTTC
CTCGAACTGGATAAAAATCACCTCAAAGGATTGTTGCTCAAAAGCGGCGGCACCACCTCA
CATACGGTGATCCTTGCCCGTTCGTTCAACATTCCAACGCTGGTTGGTGTGGATATTGAT
GCCCTTACTCCGTGGCAGCAACAAACGATTTATATCGACGGCAACGCCGGGGCGATTGTG
GTTGAGCCAGGGGAAGCCGTAGCTCGTTATTATCAGCAAGAAGCCCGCGTACAGGACGCC
CTGCGTGAGCAACAGCGTGTCTGGCTGACCCAACAAGCCCGTACCGCTGACGGTATCCGC
ATTGAAATTGCCGCTAACATCGCTCACTCCGTGGAAGCGCAGGCCGCATTCGGCAATGGT
GCGGAAGGCGTTGGTTTGTTCCGCACTGAAATGCTCTATATGGATCGCACCAGCGCACCG
GGCGAAAGCGAGTTGTACAACATTTTTTGTCAGGCGCTGGAATCCGCCAACGGACGCAGC
ATTATTGTGCGCACTATGGACATTGGCGGCGACAAACCCGTTGATTATCTGAACATTCCC
GCAGAGGCAAACCCGTTCCTCGGTTATCGCGCCGTGCGTATTTATGAAGAGTACGCGTCG
TTGTTTACCACGCAGCTACGGTCGATCCTCCGCGCCTCCGCTCACGGCAGCCTGAAAATC
ATGATCCCGATGATCTCCTCAATGGAAGAGATCTTATGGGTGAAAGAAAAACTGGCGGAA
GCCAAACAGCAACTACGTAACGAACACATTCCGTTTGATGAGAAAATCCAGCTCGGCATC
ATGCTGGAAGTGCCGTCGGTGATGTTCATCATCGATCAATGCTGCGAAGAGATTGATTTC
TTTAGTATTGGTAGTAATGACCTGACGCAGTATCTGCTGGCGGTGGATCGCGATAACGCT
AAGGTTACTCGTCACTACAACAGCCTGAATCCGGCATTCTTGCGGGCGCTCGATTACGCC
GTGCAAGCGGTGCATCGCCAGGGCAAATGGATTGGTCTGTGCGGTGAGCTGGGAGCGAAA
GGTTCCGTGCTGCCGTTGCTGGTCGGCTTAGGGCTGGATGAACTCAGCATGAGCGCACCA
TCAATTCCGGCGGCGAAAGCTCGGATGGCGCAACTTGATAGCCGTGAGTGCCGCAAGTTG
CTCAACCAGGCAATGGCCTGCCGTACTTCGCTGGAAGTAGAACACCTGCTGGCGCAATTC
CGCATGACCCAACAAGACGCACCGCTGGTCACCGCCGAGTGCATCACACTGGAAAGCGAC
TGGCGCAGCAAAGAAGAAGTGCTCAAAGGCATGACCGATAACCTGCTGCTGGCGGGCCGC
TGCCGCTATCCGCGTAAACTGGAAGCCGACTTGTGGGCGCGCGAGGCCGTTTTCTCTACC
GGTCTGGGCTTTAGTTTTGCCATTCCACACAGCAAATCAGAACACATTGAGCAATCCACC
ATCAGCGTGGCGCGTCTGCAAGCGCCGGTGCGCTGGGGCGATGATGAAGCGCAATTCATC
ATTATGTTAACCCTGAACAAACACGCTGCGGGCGATCAGCATATGCGCATTTTCTCGCGC
CTCGCTCGCCGCATCATGCACGAAGAATTCCGTAACGCGCTGGTTAACGCCGCCTCTGCC
GACGCTATCGCCAGCCTGCTGCAACATGAACTGGAACTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:833
Protein Molecular Weight:91773
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Multiphosphoryl transfer protein 2
MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLA
GDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRA
RTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALENGLTLVLKNIEFRLLDSDGA
TSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVASANHFCEEFSRSSSSYLQERALD
VRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTS
HTVILARSFNIPTLVGVDIDALTPWQQQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDA
LREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEMLYMDRTSAP
GESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYAS
LFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGI
MLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYA
VQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKL
LNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGR
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFI
IMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAASADAIASLLQHELEL
References
External Links:
ResourceLink
Uniprot ID:P32670
Uniprot Name:PTFX2_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676113
Ecogene ID:EG11906
Ecocyc:EG11906
ColiBase:b3947
Kegg Gene:b3947
EchoBASE ID:EB1851
CCDB:PTFX2_ECOLI
BacMap:49176446
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Reizer, J., Reizer, A., Saier, M. H. Jr (1995). "Novel phosphotransferase system genes revealed by bacterial genome analysis--a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system." Microbiology 141 ( Pt 4):961-971. Pubmed: 7773398
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293