Molybdopterin-guanine dinucleotide biosynthesis protein A (P32173)

Identification
Name:Molybdopterin-guanine dinucleotide biosynthesis protein A
Synonyms:
  • Protein FA
Gene Name:mobA
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Links a guanosine 5'-phosphate to molydopterin (MPT) forming molybdopterin guanine dinucleotide (MGD)
Cellular Location:Cytoplasm
SMPDB Pathways:
  • guanylyl molybdenum cofactor biosynthesis PW002032
KEGG Pathways:Not Available
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Guanylyl molybdenum cofactor+1.0Thumb
1.0Bis-molybdopterin guanine dinucleotide + 1.0Guanosine triphosphate + 1.0Hydrogen ion + 1.0Molybdopterin guanine dinucleotide → 1.0Guanylyl molybdenum cofactor + 1.0Pyrophosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0molybdenum cofactor+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0molybdenum cofactor + 1.0Guanosine triphosphate ↔ 1.0Molybdopterin guanine dinucleotide + 1.0Pyrophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Guanosine triphosphate + 1.0Hydrogen ion + 1.0Molybdopterin → 1.0Molybdopterin guanine dinucleotide + 1.0Pyrophosphate
ReactionCard
1.0tungsten bispterin cofactor+1.0Thumb+1.0Thumb → 1.0tungsten bispterin cofactor mono-guanine dinucleotide+1.0Thumb
1.0tungsten bispterin cofactor + 1.0Guanosine triphosphate + 1.0Hydrogen ion → 1.0tungsten bispterin cofactor mono-guanine dinucleotide + 1.0Pyrophosphate
ReactionCard
1.0tungsten bispterin cofactor mono-guanine dinucleotide+1.0Thumb+1.0Thumb → 1.0tungsten bispterin cofactor guanine dinucleotide+1.0Thumb
1.0tungsten bispterin cofactor mono-guanine dinucleotide + 1.0Guanosine triphosphate + 1.0Hydrogen ion → 1.0tungsten bispterin cofactor guanine dinucleotide + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21310Bis-molybdopterin guanine dinucleotideMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB02206MolybdopterinMetaboCard
ECMDB20281Molybdopterin guanine dinucleotideMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
catalytic activity
Process
cellular metabolic process
coenzyme biosynthetic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
Mo-molybdopterin cofactor biosynthetic process
Gene Properties
Blattner:b3857
Gene OrientationCounterclockwise
Centisome Percentage:87.06
Left Sequence End4039438
Right Sequence End4040022
Gene Sequence:
>585 bp
ATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTG
CGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAT
TTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAG
GCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTA
TTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGC
TCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTC
ATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGC
ATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGT
TTTGAAAAAGAAGGCTATGCGAAAGACTATCTGTTGATTGATGGACAATGGCGCGATCAC
GTACTGACGGCATTAACTACCCCAGACTGGACGCCCGGCCGCTAA
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:194
Protein Molecular Weight:21643
Protein Theoretical pI:6
PDB File:1FRW
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Molybdopterin-guanine dinucleotide biosynthesis protein A
MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALMTQLSHVVVNANRHQEI
YQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDTPYIPPDLAARLNHQRKDA
PVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMVFMRLAGGHAVDFSDHKDAFVN
VNTPEELARWQEKR
References
External Links:
ResourceLink
Uniprot ID:P32173
Uniprot Name:MOBA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651524
PDB ID:1FRW
Ecogene ID:EG11829
Ecocyc:EG11829
ColiBase:b3857
Kegg Gene:b3857
EchoBASE ID:EB1776
CCDB:MOBA_ECOLI
BacMap:16131698
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Iobbi-Nivol, C., Palmer, T., Whitty, P. W., McNairn, E., Boxer, D. H. (1995). "The mob locus of Escherichia coli K12 required for molybdenum cofactor biosynthesis is expressed at very low levels." Microbiology 141 ( Pt 7):1663-1671. Pubmed: 7551035
  • Lake, M. W., Temple, C. A., Rajagopalan, K. V., Schindelin, H. (2000). "The crystal structure of the Escherichia coli MobA protein provides insight into molybdopterin guanine dinucleotide biosynthesis." J Biol Chem 275:40211-40217. Pubmed: 10978347
  • Palmer, T., Vasishta, A., Whitty, P. W., Boxer, D. H. (1994). "Isolation of protein FA, a product of the mob locus required for molybdenum cofactor biosynthesis in Escherichia coli." Eur J Biochem 222:687-692. Pubmed: 8020507
  • Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018
  • Stevenson, C. E., Sargent, F., Buchanan, G., Palmer, T., Lawson, D. M. (2000). "Crystal structure of the molybdenum cofactor biosynthesis protein MobA from Escherichia coli at near-atomic resolution." Structure 8:1115-1125. Pubmed: 11080634