Rhamnulokinase (P32171)

Identification
Name:Rhamnulokinase
Synonyms:
  • Rhamnulose kinase
Gene Name:rhaB
Enzyme Class:
Biological Properties
General Function:Involved in phosphotransferase activity, alcohol group as acceptor
Specific Function:ATP + L-rhamnulose = ADP + L-rhamnulose 1- phosphate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Fructose and mannose metabolism ec00051
  • Microbial metabolism in diverse environments ec01120
  • Pentose and glucuronate interconversions ec00040
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Rhamnulose ↔ 1.0ADP + 1.0Hydrogen ion + 1.0L-Rhamnulose 1-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Rhamnulose ↔ 1.0ADP + 1.0L-Rhamnulose 1-phosphate
ReactionCard
SMPDB Reactions:
1.0L-rhamnulofuranose+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0L-rhamnulose 1-phosphate+1.0Thumb+1.0Thumb
1.0L-rhamnulofuranose + 1.0Adenosine triphosphate + 1.0L-rhamnulofuranose → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0L-rhamnulose 1-phosphate + 1.0ADP + 1.0L-Rhamnulose 1-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Rhamnulose ↔ 1.0ADP + 1.0Hydrogen ion + 1.0L-Rhamnulose 1-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Rhamnulose + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0L-Rhamnulose 1-phosphate + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Rhamnulose → 1.0ADP + 1.0L-Rhamnulose 1-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB24211L-rhamnulofuranoseMetaboCard
ECMDB04144L-RhamnuloseMetaboCard
ECMDB20166L-Rhamnulose 1-phosphateMetaboCard
ECMDB00751L-Threo-2-pentuloseMetaboCard
GO Classification:
Function
catalytic activity
phosphotransferase activity, alcohol group as acceptor
rhamnulokinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
alcohol metabolic process
carbohydrate metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
primary metabolic process
rhamnose catabolic process
rhamnose metabolic process
small molecule metabolic process
Gene Properties
Blattner:b3904
Gene OrientationCounterclockwise
Centisome Percentage:88.24
Left Sequence End4094002
Right Sequence End4095471
Gene Sequence:
>1470 bp
ATGACCTTTCGCAATTGTGTCGCCGTCGATCTCGGCGCATCCAGTGGGCGCGTGATGCTG
GCGCGTTACGAGCGTGAATGCCGCAGCCTGACGCTGCGCGAAATCCATCGTTTTAACAAT
GGGCTGCATAGTCAGAACGGCTATGTCACCTGGGATGTGGATAGCCTTGAAAGTGCCATT
CGCCTTGGATTAAACAAGGTGTGCGAGGAAGGGATTCGTATCGATAGCATTGGGATTGAT
ACCTGGGGCGTGGACTTTGTGCTGCTCGACCAACAGGGTCAGCGTGTGGGCCTGCCCGTT
GCTTATCGCGATAGCCGCACCAATGGCCTAATGGCGCAGGCACAACAACAACTCGGCAAA
CGCGATATTTATCAACGTAGCGGCATCCAGTTTCTGCCCTTCAATACGCTTTATCAGTTG
CGTGCGCTGACGGAGCAACAACCTGAACTTATTCCACACATTGCTCACGCTCTGCTGATG
CCGGATTACTTCAGTTATCGCCTGACCGGCAAGATGAACTGGGAATATACCAACGCCACG
ACCACGCAACTGGTCAATATCAATAGCGACGACTGGGACGAGTCGCTACTGGCGTGGAGC
GGGGCCAACAAAGCCTGGTTTGGTCGCCCGACGCATCCGGGTAATGTCATAGGTCACTGG
ATTTGCCCGCAGGGTAATGAGATTCCAGTGGTCGCCGTTGCCAGCCATGATACCGCCAGC
GCGGTTATCGCCTCGCCGTTAAACGGCTCACGTGCTGCTTATCTCTCTTCTGGCACCTGG
TCATTGATGGGCTTCGAAAGCCAGACGCCATTTACCAATGACACGGCACTGGCAGCCAAC
ATCACCAATGAAGGCGGGGCGGAAGGTCGCTATCGGGTGCTGAAAAATATTATGGGCTTA
TGGCTGCTTCAGCGAGTGCTTCAGGAGCAGCAAATCAACGATCTTCCGGCGCTTATCTCC
GCGACACAGGCACTTCCGGCTTGCCGCTTCATTATCAATCCCAATGACGATCGCTTTATT
AATCCTGAGACGATGTGCAGCGAAATTCAGGCTGCGTGTCGGGAAACGGCGCAACCGATC
CCGGAAAGTGATGCTGAACTGGCGCGCTGCATTTTCGACAGTCTGGCGCTGCTGTATGCC
GATGTGTTGCATGAGCTGGCGCAGCTGCGCGGTGAAGATTTCTCGCAACTGCATATTGTC
GGCGGAGGCTGCCAGAACACGCTGCTCAACCAGCTATGCGCCGATGCCTGCGGTATTCGG
GTGATCGCCGGGCCTGTTGAAGCCTCGACGCTCGGCAATATCGGCATCCAGTTAATGACG
CTGGATGAACTCAACAATGTGGATGATTTCCGTCAGGTCGTCAGCACCACCGCGAATCTG
ACCACCTTTACCCCTAATCCTGACAGTGAAATTGCCCACTATGTGGCGCAGATTCACTCT
ACACGACAGACAAAGGAGCTTTGCGCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:489
Protein Molecular Weight:54069
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Rhamnulokinase
MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHSQNGYVTWDVDSLESAI
RLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAYRDSRTNGLMAQAQQQLGK
RDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLMPDYFSYRLTGKMNWEYTNAT
TTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIGHWICPQGNEIPVVAVASHDTAS
AVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTALAANITNEGGAEGRYRVLKNIMGL
WLLQRVLQEQQINDLPALISATQALPACRFIINPNDDRFINPETMCSEIQAACRETAQPI
PESDAELARCIFDSLALLYADVLHELAQLRGEDFSQLHIVGGGCQNTLLNQLCADACGIR
VIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDSEIAHYVAQIHS
TRQTKELCA
References
External Links:
ResourceLink
Uniprot ID:P32171
Uniprot Name:RHAB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676155
Ecogene ID:EG11868
Ecocyc:EG11868
ColiBase:b3904
Kegg Gene:b3904
EchoBASE ID:EB1814
CCDB:RHAB_ECOLI
BacMap:16131744
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Egan, S. M., Schleif, R. F. (1993). "A regulatory cascade in the induction of rhaBAD." J Mol Biol 234:87-98. Pubmed: 8230210
  • Grueninger, D., Schulz, G. E. (2006). "Structure and reaction mechanism of L-rhamnulose kinase from Escherichia coli." J Mol Biol 359:787-797. Pubmed: 16674975
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Holcroft, C. C., Egan, S. M. (2000). "Roles of cyclic AMP receptor protein and the carboxyl-terminal domain of the alpha subunit in transcription activation of the Escherichia coli rhaBAD operon." J Bacteriol 182:3529-3535. Pubmed: 10852886
  • Moralejo, P., Egan, S. M., Hidalgo, E., Aguilar, J. (1993). "Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli." J Bacteriol 175:5585-5594. Pubmed: 8396120
  • Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018