Identification
Name:Molybdopterin-guanine dinucleotide biosynthesis protein B
Synonyms:Not Available
Gene Name:mobB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in GTP binding
Specific Function:May bind the guanine nucleotide required for the synthesis of molybdopterin guanine dinucleotide
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0tungsten bispterin cofactor+1.0Thumb+1.0Thumb1.0tungsten bispterin cofactor mono-guanine dinucleotide+1.0Thumb
1.0tungsten bispterin cofactor + 1.0Guanosine triphosphate + 1.0Hydrogen ion → 1.0tungsten bispterin cofactor mono-guanine dinucleotide + 1.0Pyrophosphate
ReactionCard
1.0tungsten bispterin cofactor mono-guanine dinucleotide+1.0Thumb+1.0Thumb1.0tungsten bispterin cofactor guanine dinucleotide+1.0Thumb
1.0tungsten bispterin cofactor mono-guanine dinucleotide + 1.0Guanosine triphosphate + 1.0Hydrogen ion → 1.0tungsten bispterin cofactor guanine dinucleotide + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21310Bis-molybdopterin guanine dinucleotideMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB02206MolybdopterinMetaboCard
ECMDB20281Molybdopterin guanine dinucleotideMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
binding
GTP binding
guanyl nucleotide binding
guanyl ribonucleotide binding
nucleotide binding
purine nucleotide binding
Process
cellular metabolic process
coenzyme biosynthetic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
Mo-molybdopterin cofactor biosynthetic process
Gene Properties
Blattner:b3856
Gene OrientationCounterclockwise
Centisome Percentage:87.05
Left Sequence End4038929
Right Sequence End4039456
Gene Sequence:
>513 bp
ATGATACCGTTACTCGCCTTTGCCGCGTGGAGTGGTACCGGAAAAACTACGCTTTTGAAA
AAACTGATCCCGGCATTATGCGCCAGAGGGATCCGTCCAGGGCTGATTAAGCATACGCAC
CATGATATGGATGTTGATAAGCCAGGCAAAGATAGCTATGAGCTGCGCAAGGCTGGCGCG
GCACAAACCATCGTTGCCAGCCAGCAACGATGGGCCTTGATGACGGAAACACCAGACGAA
GAAGAGCTGGATCTACAGTTTCTCGCAAGTCGAATGGATACCTCAAAGCTGGATTTGATT
CTGGTCGAAGGGTTTAAGCATGAAGAGATCGCAAAGATTGTGCTGTTTCGCGATGGAGCC
GGACATCGACCTGAAGAATTAGTGATAGACAGGCATGTTATTGCTGTAGCCAGTGATGTG
CCGCTTAATCTTGATGTCGCATTACTGGATATTAATGATGTTGAGGGGCTGGCTGATTTT
GTGGTGGAGTGGATGCAAAAGCAGAACGGATAA
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:175
Protein Molecular Weight:19363
Protein Theoretical pI:5
PDB File:1NP6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Molybdopterin-guanine dinucleotide biosynthesis protein B
MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHTHHDMDVDKPGKDSYEL
RKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKLDLILVEGFKHEEIAKIVL
FRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVEGLADFVVEWMQKQNG
References
External Links:
ResourceLink
Uniprot ID:P32125
Uniprot Name:MOBB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676202
PDB ID:1NP6
Ecogene ID:EG11828
Ecocyc:EG11828
ColiBase:b3856
Kegg Gene:b3856
EchoBASE ID:EB1775
CCDB:MOBB_ECOLI
BacMap:145698333
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Eaves, D. J., Palmer, T., Boxer, D. H. (1997). "The product of the molybdenum cofactor gene mobB of Escherichia coli is a GTP-binding protein." Eur J Biochem 246:690-697. Pubmed: 9219527
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Iobbi-Nivol, C., Palmer, T., Whitty, P. W., McNairn, E., Boxer, D. H. (1995). "The mob locus of Escherichia coli K12 required for molybdenum cofactor biosynthesis is expressed at very low levels." Microbiology 141 ( Pt 7):1663-1671. Pubmed: 7551035
  • Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018