GDP-L-fucose synthase (P32055)

Identification
Name:GDP-L-fucose synthase
Synonyms:
  • GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase
Gene Name:fcl
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism II PW000887
  • Mannose Metabolism PW000822
  • colanic acid building blocks biosynthesis PW000951
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0GDP-L-Fucose + 1.0NADP ↔ 1.0GDP-4-Dehydro-6-deoxy-D-mannose + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0GDP-L-Fucose + 1.0NADP → 1.0GDP-4-Dehydro-6-deoxy-D-mannose + 1.0NADPH
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0NADPH+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0GDP-4-Dehydro-6-deoxy-D-mannose + 1.0NADPH + 1.0Hydrogen ion + 1.0NADPH → 1.0GDP-L-Fucose + 1.0NADP
ReactionCard
1.0Thumb+1.0NADPH+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0GDP-4-dehydro-6-deoxy-α-D-mannose + 1.0NADPH + 1.0Hydrogen ion + 1.0NADPH → 1.0NADP + 1.0GDP-β-L-fucose
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0GDP-L-Fucose + 1.0NADP ↔ 1.0GDP-4-Dehydro-6-deoxy-D-mannose + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0GDP-L-Fucose + 1.0NADP → 1.0GDP-4-Dehydro-6-deoxy-D-mannose + 1.0NADPH
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0GDP-4-Dehydro-6-deoxy-D-mannose → 1.0GDP-4-Oxo-L-fucose
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0GDP-4-Oxo-L-fucose + 1.0Hydrogen ion + 1.0NADPH → 1.0GDP-L-Fucose + 1.0NADP
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01346GDP-4-Dehydro-6-deoxy-D-mannoseMetaboCard
ECMDB24242GDP-4-dehydro-6-deoxy-α-D-mannoseMetaboCard
ECMDB01391GDP-4-Dehydro-6-L-deoxygalactoseMetaboCard
ECMDB21219GDP-4-Oxo-L-fucoseMetaboCard
ECMDB01095GDP-L-FucoseMetaboCard
ECMDB24243GDP-β-L-fucoseMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Function
binding
catalytic activity
coenzyme binding
cofactor binding
Process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b2052
Gene OrientationCounterclockwise
Centisome Percentage:45.78
Left Sequence End2124249
Right Sequence End2125214
Gene Sequence:
>966 bp
ATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATG
AGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAG
AAAATGCTGGAAGCGGGCGAAAGTGCGCTGGATGTGGTGACGGAAGCGGTGCGTCTGCTG
GAAGAGTGTCCACTGTTTAACGCCGGAATTGGCGCTGTCTTTACGCGTGATGAAACCCAT
GAACTGGACGCCTGTGTGATGGATGGTAACACCCTGAAAGCCGGTGCGGTGGCGGGCGTT
AGTCATCTGCGTAATCCGGTTCTTGCCGCCCGGCTGGTGATGGAGCAAAGCCCGCATGTG
ATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCG
CCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGG
GCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTG
GGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATG
ACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCC
AATAACGCCAGTGTGGCGGTTTCTTGTACCGGCACGGGCGAAGTCTTCATCCGCGCGCTG
GCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGC
GAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATC
GACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGC
TACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACA
CAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:321
Protein Molecular Weight:36141
Protein Theoretical pI:7
PDB File:1FXS
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>GDP-L-fucose synthase
MSKQRVFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDSRAVHDFFASERIDQVY
LAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKLLFLGSSCIYPKLAKQPMA
ESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGPHDNFHPSNSHV
IPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMAAASIHVMELAHEVWLENTQPML
SHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASKPDGTPRKLLDVTRLHQLGWYHEIS
LEAGLASTYQWFLENQDRFRG
References
External Links:
ResourceLink
Uniprot ID:P32055
Uniprot Name:FCL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062400
PDB ID:1FXS
Ecogene ID:EG11788
Ecocyc:EG11788
ColiBase:b2052
Kegg Gene:b2052
EchoBASE ID:EB1736
CCDB:FCL_ECOLI
BacMap:16129992
General Reference:
  • Andrianopoulos, K., Wang, L., Reeves, P. R. (1998). "Identification of the fucose synthetase gene in the colanic acid gene cluster of Escherichia coli K-12." J Bacteriol 180:998-1001. Pubmed: 9473059
  • Aoyama, K., Haase, A. M., Reeves, P. R. (1994). "Evidence for effect of random genetic drift on G+C content after lateral transfer of fucose pathway genes to Escherichia coli K-12." Mol Biol Evol 11:829-838. Pubmed: 7815923
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Somers, W. S., Stahl, M. L., Sullivan, F. X. (1998). "GDP-fucose synthetase from Escherichia coli: structure of a unique member of the short-chain dehydrogenase/reductase family that catalyzes two distinct reactions at the same active site." Structure 6:1601-1612. Pubmed: 9862812
  • Stevenson, G., Andrianopoulos, K., Hobbs, M., Reeves, P. R. (1996). "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid." J Bacteriol 178:4885-4893. Pubmed: 8759852