ECMDB

Identification

Name:

Crotonobetainyl-CoA:carnitine CoA-transferase

Synonyms:

Not Available

Gene Name:

caiB

Enzyme Class:

2.8.3.21

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Catalyzes the reversible transfer of the CoA moiety from gamma-butyrobetainyl-CoA to L-carnitine to generate L-carnitinyl- CoA and gamma-butyrobetaine. Is also able to catalyze the reversible transfer of the CoA moiety from gamma-butyrobetainyl- CoA or L-carnitinyl-CoA to crotonobetaine to generate crotonobetainyl-CoA

Cellular Location:

Cytoplasm

SMPDB Pathways:

L-carnitine degradation I PW002037
Operon: 16S ribosomal RNA modification & chaperone PW001877
Operon: probable carnitine operon PW001875

KEGG Pathways:

Not Available

KEGG Reactions:

1.0

↔

1.0

1.0(E)-4-(Trimethylammonio)but-2-enoyl-CoA + 1.0L-Carnitine + 1.04-Trimethylammoniobutanoyl-CoA ↔ 1.0Crotonobetaine + 1.0L-Carnitinyl-CoA + 1.0gamma-Butyrobetaine
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0gamma-Butyrobetainyl-CoA + 1.0L-Carnitine → 1.0D-Carnitinyl-CoA + 1.0gamma-Butyrobetaine
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0gamma-Butyrobetainyl-CoA + 1.0Carnitine ↔ 1.0L-Carnitinyl-CoA + 1.0gamma-Butyrobetaine
ReactionCard

Complex Reactions:

1.0

↔

1.0

1.0Carnitine + 1.0Crotonobetainyl-CoA ↔ 1.0L-Carnitinyl-CoA + 1.0Crotonobetaine
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB23848	(E)-4-(Trimethylammonio)but-2-enoyl-CoA	MetaboCard
ECMDB23901	4-Trimethylammoniobutanoyl-CoA	MetaboCard
ECMDB00062	Carnitine	MetaboCard
ECMDB21321	Crotonobetaine	MetaboCard
ECMDB20539	Crotonobetainyl-CoA	MetaboCard
ECMDB20541	D-Carnitinyl-CoA	MetaboCard
ECMDB21336	gamma-Butyrobetaine	MetaboCard
ECMDB20559	gamma-Butyrobetainyl-CoA	MetaboCard
ECMDB23900	L-Carnitine	MetaboCard
ECMDB20578	L-Carnitinyl-CoA	MetaboCard

GO Classification:

Function
catalytic activity
Process
metabolic process

Gene Properties

Blattner:

b0038

Gene Orientation

Counterclockwise

Centisome Percentage:

0.82

Left Sequence End

37898

Right Sequence End

39115

Gene Sequence:

>1218 bp
ATGGATCATCTACCCATGCCGAAATTCGGGCCGTTGGCCGGATTGCGCGTTGTCTTCTCC
GGTATCGAAATCGCCGGACCGTTTGCCGGGCAAATGTTCGCAGAATGGGGCGCGGAAGTT
ATCTGGATCGAGAACGTCGCCTGGGCCGACACCATTCGCGTTCAACCGAACTACCCGCAA
CTCTCCCGCCGCAATTTGCACGCGCTGTCGTTAAATATTTTCAAAGATGAAGGCCGCGAA
GCGTTTCTGAAATTAATGGAAACCACCGATATCTTCATCGAAGCCAGTAAAGGTCCGGCC
TTTGCCCGTCGTGGCATTACCGATGAAGTACTGTGGCAGCACAACCCGAAACTGGTTATC
GCTCACCTGTCCGGTTTTGGTCAGTACGGCACCGAGGAGTACACCAATCTTCCGGCCTAT
AACACTATCGCCCAGGCCTTTAGTGGTTACCTGATTCAGAACGGTGATGTTGACCAGCCA
ATGCCTGCCTTCCCGTATACCGCCGATTACTTTTCTGGCCTGACCGCCACCACGGCGGCG
CTGGCAGCACTGCATAAAGTGCGTGAAACCGGTAAAGGCGAAAGTATCGACATCGCCATG
TATGAAGTGATGCTGCGTATGGGCCAGTACTTCATGATGGATTACTTCAACGGCGGCGAA
ATGTGCCCGCGCATGAGCAAAGGTAAAGATCCCTACTACGCCGGTTGCGGTCTGTATAAA
TGTGCCGACGGCTACATCGTGATGGAACTGGTGGGCATTACCCAAATTGAAGAGTGCTTT
AAAGATATTGGCCTCGCACATCTGCTTGGCACGCCAGAAATCCCGGAAGGCACTCAGCTT
ATCCACCGTATCGAATGCCCTTACGGCCCACTGGTTGAAGAGAAACTCGATGCCTGGCTG
GCGACACATACCATCGCGGAAGTAAAAGAACGCTTTGCTGAACTGAATATCGCCTGCGCC
AAAGTGCTGACCGTACCGGAACTGGAAAGCAATCCACAGTATGTGGCTCGCGAATCAATC
ACTCAGTGGCAAACGATGGATGGTCGCACCTGCAAAGGGCCGAACATCATGCCGAAATTC
AAAAATAACCCCGGACAAATCTGGCGCGGAATGCCCTCACATGGCATGGACACGGCTGCC
ATTTTGAAAAATATCGGCTACAGCGAAAACGACATTCAGGAGTTGGTCAGCAAAGGTCTG
GCCAAAGTTGAGGACTAA

Protein Properties

Pfam Domain Function:

CoA_transf_3 (PF02515 )

Protein Residues:

405

Protein Molecular Weight:

45126

Protein Theoretical pI:

PDB File:

1XVV

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Crotonobetainyl-CoA:carnitine CoA-transferase
MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIENVAWADTIRVQPNYPQ
LSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFARRGITDEVLWQHNPKLVI
AHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQPMPAFPYTADYFSGLTATTAA
LAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNGGEMCPRMSKGKDPYYAGCGLYK
CADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPEGTQLIHRIECPYGPLVEEKLDAWL
ATHTIAEVKERFAELNIACAKVLTVPELESNPQYVARESITQWQTMDGRTCKGPNIMPKF
KNNPGQIWRGMPSHGMDTAAILKNIGYSENDIQELVSKGLAKVED

References

External Links:

Resource	Link
Uniprot ID:	P31572
Uniprot Name:	CAIB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21321920
PDB ID:	1XVV
Ecogene ID:	EG11559
Ecocyc:	EG11559
ColiBase:	b0038
Kegg Gene:	b0038
EchoBASE ID:	EB1520
CCDB:	CAIB_ECOLI
BacMap:	16128032

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Eichler, K., Bourgis, F., Buchet, A., Kleber, H. P., Mandrand-Berthelot, M. A. (1994). "Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli." Mol Microbiol 13:775-786. Pubmed: 7815937
Eichler, K., Schunck, W. H., Kleber, H. P., Mandrand-Berthelot, M. A. (1994). "Cloning, nucleotide sequence, and expression of the Escherichia coli gene encoding carnitine dehydratase." J Bacteriol 176:2970-2975. Pubmed: 8188598
Elssner, T., Engemann, C., Baumgart, K., Kleber, H. P. (2001). "Involvement of coenzyme A esters and two new enzymes, an enoyl-CoA hydratase and a CoA-transferase, in the hydration of crotonobetaine to L-carnitine by Escherichia coli." Biochemistry 40:11140-11148. Pubmed: 11551212
Elssner, T., Preusser, A., Wagner, U., Kleber, H. P. (1999). "Metabolism of L(-)-carnitine by Enterobacteriaceae under aerobic conditions." FEMS Microbiol Lett 174:295-301. Pubmed: 10339822
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Stenmark, P., Gurmu, D., Nordlund, P. (2004). "Crystal structure of CaiB, a type-III CoA transferase in carnitine metabolism." Biochemistry 43:13996-14003. Pubmed: 15518548
Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901

Crotonobetainyl-CoA:carnitine CoA-transferase (P31572)