Carnitinyl-CoA dehydratase (P31551)

Identification
Name:Carnitinyl-CoA dehydratase
Synonyms:
  • Crotonobetainyl-CoA hydratase
Gene Name:caiD
Enzyme Class:
Biological Properties
General Function:Involved in hydro-lyase activity
Specific Function:Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Carnitinyl-CoA ↔ 1.0(E)-4-(Trimethylammonio)but-2-enoyl-CoA + 1.0Water
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.0D-Carnitinyl-CoA → 1.0Crotonobetainyl-CoA + 1.0Water
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Carnitinyl-CoA ↔ 1.0Crotonobetainyl-CoA + 1.0Water
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Carnitinyl-CoA → 1.0Crotonobetainyl-CoA + 1.0Water
ReactionCard
Complex Reactions:
1.0Thumb ↔ 1.0Thumb
1.0L-Carnitinyl-CoA ↔ 1.0D-Carnitinyl-CoA
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23848(E)-4-(Trimethylammonio)but-2-enoyl-CoAMetaboCard
ECMDB20539Crotonobetainyl-CoAMetaboCard
ECMDB20541D-Carnitinyl-CoAMetaboCard
ECMDB20578L-Carnitinyl-CoAMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
Process
metabolic process
Gene Properties
Blattner:b0036
Gene OrientationCounterclockwise
Centisome Percentage:0.76
Left Sequence End35377
Right Sequence End36162
Gene Sequence:
>786 bp
ATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCC
TCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAAT
AAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCC
GTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAAT
TCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAG
ATGCGAAAATATTATACTGTTTCCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTG
ACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATG
AACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTT
TACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGAAATCAACAGTATGGTCC
GACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACG
CTGGTGGATGTGGCAACAGGTGAGTGGGCTACCTGGTCGCCGGTGAATTATGAATATAAA
GTGCTTCCACCATTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAA
ATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTAT
CAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:261
Protein Molecular Weight:28190
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Carnitinyl-CoA dehydratase
MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFF
SAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIV
CADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVMTGRRMGAEEALRWGIVNRVV
SQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHS
EDAIEGPLAFAEKRDPVWKGR
References
External Links:
ResourceLink
Uniprot ID:P31551
Uniprot Name:CAID_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902953
Ecogene ID:EG11557
Ecocyc:EG11557
ColiBase:b0036
Kegg Gene:b0036
EchoBASE ID:EB1518
CCDB:CAID_ECOLI
BacMap:221800779
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Eichler, K., Bourgis, F., Buchet, A., Kleber, H. P., Mandrand-Berthelot, M. A. (1994). "Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli." Mol Microbiol 13:775-786. Pubmed: 7815937
  • Elssner, T., Engemann, C., Baumgart, K., Kleber, H. P. (2001). "Involvement of coenzyme A esters and two new enzymes, an enoyl-CoA hydratase and a CoA-transferase, in the hydration of crotonobetaine to L-carnitine by Escherichia coli." Biochemistry 40:11140-11148. Pubmed: 11551212
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901