Thiamine import ATP-binding protein ThiQ (P31548)

Identification
Name:Thiamine import ATP-binding protein ThiQ
Synonyms:Not Available
Gene Name:thiQ
Enzyme Class:Not Available
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex ThiBPQ involved in thiamine import. Responsible for energy coupling to the transport system (Probable)
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Thiamine → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Thiamine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00235ThiamineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cell wall
external encapsulating structure
Gram-negative-bacterium-type cell wall
peptidoglycan-based cell wall
Function
active transmembrane transporter activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
ATPase activity, coupled to transmembrane movement of substances
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
P-P-bond-hydrolysis-driven transmembrane transporter activity
primary active transmembrane transporter activity
purine nucleoside binding
pyrophosphatase activity
transmembrane transporter activity
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b0066
Gene OrientationCounterclockwise
Centisome Percentage:1.56
Left Sequence End72229
Right Sequence End72927
Gene Sequence:
>699 bp
ATGTTAAAACTGACTGATATCACCTGGCTTTACCACCATTTGCCGATGCGTTTTAGCTTA
ACGGTGGAACGCGGCGAGCAGGTGGCGATCCTCGGGCCAAGCGGCGCGGGTAAAAGTACC
CTGCTGAATTTGATCGCCGGTTTTCTGACGCCAGCCAGCGGTTCGCTGACTATCGATGGC
GTAGATCACACAACTATGCCGCCGTCACGCCGTCCGGTGTCGATGCTGTTTCAGGAGAAC
AACCTGTTCAGCCACCTGACGGTCGCACAGAACATCGGGCTGGGGCTAAATCCGGGATTG
AAACTGAACGCGGTACAGCAGGGGAAAATGCACGCTATCGCCCGCCAGATGGGGATTGAT
AATTTAATGGCGCGGTTACCGGGCGAGCTTTCCGGCGGTCAGCGACAGCGAGTGGCGTTA
GCGCGTTGTCTGGTACGCGAACAGCCGATTTTATTGCTCGATGAACCGTTCTCTGCGCTC
GATCCGGCGTTACGTCAGGAGATGTTGACGCTGGTGAGCACGAGCTGCCAGCAGCAAAAA
ATGACGCTATTGATGGTGTCGCACAGCGTGGAAGATGCGGCGCGGATCGCCACGCGCTCG
GTAGTAGTCGCCGACGGGCGCATCGCCTGGCAGGGTATGACCAATGAGTTGTTGAGCGGT
AAGGCGAGTGCTTCGGCACTATTGGGGATTACGGGTTAG
Protein Properties
Pfam Domain Function:
Protein Residues:232
Protein Molecular Weight:24999
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Thiamine import ATP-binding protein ThiQ
MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNLIAGFLTPASGSLTIDG
VDHTTMPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKLNAVQQGKMHAIARQMGID
NLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSALDPALRQEMLTLVSTSCQQQK
MTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELLSGKASASALLGITG
References
External Links:
ResourceLink
Uniprot ID:P31548
Uniprot Name:THIQ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674311
Ecogene ID:EG11572
Ecocyc:EG11572
ColiBase:b0066
Kegg Gene:b0066
EchoBASE ID:EB1532
CCDB:THIQ_ECOLI
BacMap:16128060
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hollenbach, A. D., Dickson, K. A., Washabaugh, M. W. (2002). "Thiamine transport in Escherichia coli: the mechanism of inhibition by the sulfhydryl-specific modifier N-ethylmaleimide." Biochim Biophys Acta 1564:421-428. Pubmed: 12175925
  • Lenski, R. E., Winkworth, C. L., Riley, M. A. (2003). "Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations." J Mol Evol 56:498-508. Pubmed: 12664169
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901