| Identification |
|---|
| Name: | 2,5-diketo-D-gluconic acid reductase B |
|---|
| Synonyms: | - 2,5-DKG reductase B
- 2,5-DKGR B
- 25DKGR-B
- AKR5D
|
|---|
| Gene Name: | dkgB |
|---|
| Enzyme Class: | |
|---|
| Biological Properties |
|---|
| General Function: | Involved in oxidoreductase activity |
|---|
| Specific Function: | Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) |
|---|
| Cellular Location: | Cytoplasm |
|---|
| SMPDB Pathways: | |
|---|
| KEGG Pathways: | Not Available |
|---|
| KEGG Reactions: | |
|---|
| SMPDB Reactions: | |
1.0 | + | 1.0 | + | 1.0NADPH | + | 1.0 | → | 1.0 | + | 1.02-Keto-L-gluconate | + | 1.0 |
| | |
1.0 | + | 1.0 | + | 1.0 | ↔ | 1.0Hydroxyacetone | + | 1.0 |
| | | |
|
|---|
| EcoCyc Reactions: | |
|---|
| Complex Reactions: | |
1.0 | + | 1.0 | + | 1.0 | → | 2.0-Dehydro-L-gulonate | + | 1.0 |
| | |
1.0 | + | 1.0 | + | 1.0 | → | 1.0Acetol | + | 1.0 |
| |
|
|---|
| Metabolites: | |
|---|
| GO Classification: | | Function |
|---|
| catalytic activity | | oxidoreductase activity | | Process |
|---|
| metabolic process | | oxidation reduction |
|
|---|
| Gene Properties |
|---|
| Blattner: | b0207 |
|---|
| Gene Orientation | Clockwise |
|---|
| Centisome Percentage: | 4.94 |
|---|
| Left Sequence End | 229167 |
|---|
| Right Sequence End | 229970 |
|---|
| Gene Sequence: | >804 bp
ATGGCTATCCCTGCATTTGGTTTAGGTACTTTCCGTCTGAAAGACGACGTTGTTATTTCA
TCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGAT
AACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGGCGTGCCACGTCATGAACTCTAC
ATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAA
GAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCA
CCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAA
GGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATT
GCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAA
AACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACG
CTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAAT
GCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGAAGGTTACTCAGTAATTCCT
TCTTCTACTAAACGTAAAAACCTGGAAAGTAATCTTAAGGCACAAAATTTACAGCTTGAT
GCCGAAGATAAAAAAGCGATCGCCGCACTGGATTGCAACGACCGCCTGGTTAGCCCGGAA
GGTCTGGCTCCTGAATGGGATTAA |
|---|
| Protein Properties |
|---|
| Pfam Domain Function: | |
|---|
| Protein Residues: | 267 |
|---|
| Protein Molecular Weight: | 29437 |
|---|
| Protein Theoretical pI: | 5 |
|---|
| Signaling Regions: | |
|---|
| Transmembrane Regions: | |
|---|
| Protein Sequence: | >2,5-diketo-D-gluconic acid reductase B
MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELY
ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQ
GLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT
LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD
AEDKKAIAALDCNDRLVSPEGLAPEWD |
|---|
| References |
|---|
| External Links: | |
|---|
| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Sekiya, T., Mori, M., Takahashi, N., Nishimura, S. (1980). "Sequence of the distal tRNA1Asp gene and the transcription termination signal in the Escherichia coli ribosomal RNA operon rrnF(or G)." Nucleic Acids Res 8:3809-3827. Pubmed: 6255418
- Yum, D. Y., Lee, B. Y., Pan, J. G. (1999). "Identification of the yqhE and yafB genes encoding two 2, 5-diketo-D-gluconate reductases in Escherichia coli." Appl Environ Microbiol 65:3341-3346. Pubmed: 10427017
|
|---|
