2,5-diketo-D-gluconic acid reductase B (P30863)

Identification
Name:2,5-diketo-D-gluconic acid reductase B
Synonyms:
  • 2,5-DKG reductase B
  • 2,5-DKGR B
  • 25DKGR-B
  • AKR5D
Gene Name:dkgB
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG)
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0NADP ↔ 1.02,5-Diketo-D-gluconate + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Dehydro-D-gluconate + 1.0NADP ↔ 1.02,5-Diketo-D-gluconate + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0NADPH+1.0Thumb → 1.0Thumb+1.02-Keto-L-gluconate+1.0Thumb
1.02,5-Diketo-D-gluconate + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.02-Keto-L-gluconate + 1.02-Dehydro-D-gluconate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Hydroxyacetone+1.0Thumb
1.0Pyruvaldehyde + 1.0NADPH + 1.0Hydrogen ion ↔ 1.0Hydroxyacetone + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Pyruvaldehyde + 1.0NADPH + 1.0Hydrogen ion → 1.0Lactaldehyde + 1.0NADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0NADPH + 1.02,5-Diketo-D-gluconate ↔ 1.02-Dehydro-D-gluconate + 1.0NADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 2.0-Dehydro-L-gulonate+1.0Thumb
1.02,5-Diketo-D-gluconate + 1.0Hydrogen ion + 1.0NADPH → 2.0-Dehydro-L-gulonate + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Acetol+1.0Thumb
1.0Hydrogen ion + 1.0Pyruvaldehyde + 1.0NADPH → 1.0Acetol + 1.0NADP
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB040622,5-Diketo-D-gluconateMetaboCard
ECMDB040422-Dehydro-D-gluconateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB03052LactaldehydeMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB01167PyruvaldehydeMetaboCard
GO Classification:
Function
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b0207
Gene OrientationClockwise
Centisome Percentage:4.94
Left Sequence End229167
Right Sequence End229970
Gene Sequence:
>804 bp
ATGGCTATCCCTGCATTTGGTTTAGGTACTTTCCGTCTGAAAGACGACGTTGTTATTTCA
TCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGAT
AACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGGCGTGCCACGTCATGAACTCTAC
ATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAA
GAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCA
CCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAA
GGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATT
GCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAA
AACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACG
CTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAAT
GCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGAAGGTTACTCAGTAATTCCT
TCTTCTACTAAACGTAAAAACCTGGAAAGTAATCTTAAGGCACAAAATTTACAGCTTGAT
GCCGAAGATAAAAAAGCGATCGCCGCACTGGATTGCAACGACCGCCTGGTTAGCCCGGAA
GGTCTGGCTCCTGAATGGGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:267
Protein Molecular Weight:29437
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2,5-diketo-D-gluconic acid reductase B
MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELY
ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQ
GLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT
LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD
AEDKKAIAALDCNDRLVSPEGLAPEWD
References
External Links:
ResourceLink
Uniprot ID:P30863
Uniprot Name:DKGB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674382
Ecogene ID:EG11648
Ecocyc:EG11648
ColiBase:b0207
Kegg Gene:b0207
EchoBASE ID:EB1601
CCDB:DKGB_ECOLI
BacMap:16128194
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sekiya, T., Mori, M., Takahashi, N., Nishimura, S. (1980). "Sequence of the distal tRNA1Asp gene and the transcription termination signal in the Escherichia coli ribosomal RNA operon rrnF(or G)." Nucleic Acids Res 8:3809-3827. Pubmed: 6255418
  • Yum, D. Y., Lee, B. Y., Pan, J. G. (1999). "Identification of the yqhE and yafB genes encoding two 2, 5-diketo-D-gluconate reductases in Escherichia coli." Appl Environ Microbiol 65:3341-3346. Pubmed: 10427017