DNA polymerase III subunit psi (P28632)

Identification
Name:DNA polymerase III subunit psi
Synonyms:Not Available
Gene Name:holD
Enzyme Class:
Biological Properties
General Function:Involved in DNA-directed DNA polymerase activity
Specific Function:DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The exact function of the psi subunit is unknown
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dATP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dGTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dCTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0Thymidine 5'-triphosphate + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Deoxynucleoside triphosphate+1.0DNA ↔ 1.0Thumb
1.0Deoxynucleoside triphosphate + 1.0DNA ↔ 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01532dATPMetaboCard
ECMDB00998dCTPMetaboCard
ECMDB01440dGTPMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB01342Thymidine 5'-triphosphateMetaboCard
GO Classification:
Function
3'-5' exonuclease activity
catalytic activity
DNA polymerase activity
DNA-directed DNA polymerase activity
exonuclease activity
hydrolase activity
hydrolase activity, acting on ester bonds
nuclease activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA replication
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b4372
Gene OrientationClockwise
Centisome Percentage:99.27
Left Sequence End4605826
Right Sequence End4606239
Gene Sequence:
>414 bp
ATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATG
GTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCGATGATGTGCTGGAAGCCGAAATG
CCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCG
CTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTG
GTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGC
GTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGAT
GAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGA
Protein Properties
Pfam Domain Function:
Protein Residues:137
Protein Molecular Weight:15174
Protein Theoretical pI:6
PDB File:1EM8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA polymerase III subunit psi
MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVANDLPALTDPLVSDVLRA
LTVSPDQVLQLTPEKIAMLPQGSHCNSWRLGTDEPLSLEGAQVASPALTDLRANPTARAA
LWQQICTYEHDFFPRND
References
External Links:
ResourceLink
Uniprot ID:P28632
Uniprot Name:HOLD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674718
PDB ID:1EM8
Ecogene ID:EG11414
Ecocyc:EG11414
ColiBase:b4372
Kegg Gene:b4372
EchoBASE ID:EB1386
CCDB:HOLD_ECOLI
BacMap:16132190
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Carter, J. R., Franden, M. A., Aebersold, R., McHenry, C. S. (1993). "Identification, isolation, and overexpression of the gene encoding the psi subunit of DNA polymerase III holoenzyme." J Bacteriol 175:5604-5610. Pubmed: 8366044
  • Gulbis, J. M., Kazmirski, S. L., Finkelstein, J., Kelman, Z., O'Donnell, M., Kuriyan, J. (2004). "Crystal structure of the chi:psi sub-assembly of the Escherichia coli DNA polymerase clamp-loader complex." Eur J Biochem 271:439-449. Pubmed: 14717711
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • O'Donnell, M. (1992). "Accessory protein function in the DNA polymerase III holoenzyme from E. coli." Bioessays 14:105-111. Pubmed: 1575709
  • Xiao, H., Crombie, R., Dong, Z., Onrust, R., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi." J Biol Chem 268:11773-11778. Pubmed: 8389364
  • Xiao, H., Dong, Z., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. IV. Characterization of chi and psi." J Biol Chem 268:11779-11784. Pubmed: 8505305
  • Yoshikawa, A., Isono, S., Sheback, A., Isono, K. (1987). "Cloning and nucleotide sequencing of the genes rimI and rimJ which encode enzymes acetylating ribosomal proteins S18 and S5 of Escherichia coli K12." Mol Gen Genet 209:481-488. Pubmed: 2828880