DNA polymerase III subunit delta (P28630)

Identification
Name:DNA polymerase III subunit delta
Synonyms:Not Available
Gene Name:holA
Enzyme Class:
Biological Properties
General Function:Involved in DNA binding
Specific Function:DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the beta subunit on the DNA
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dATP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dGTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dCTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0Thymidine 5'-triphosphate + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Deoxynucleoside triphosphate+1.0DNA ↔ 1.0Thumb
1.0Deoxynucleoside triphosphate + 1.0DNA ↔ 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01532dATPMetaboCard
ECMDB00998dCTPMetaboCard
ECMDB01440dGTPMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB01342Thymidine 5'-triphosphateMetaboCard
GO Classification:
Component
DNA polymerase complex
DNA polymerase III complex
macromolecular complex
protein complex
Function
binding
catalytic activity
DNA binding
DNA polymerase activity
DNA-directed DNA polymerase activity
nucleic acid binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA replication
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b0640
Gene OrientationCounterclockwise
Centisome Percentage:14.44
Left Sequence End669797
Right Sequence End670828
Gene Sequence:
>1032 bp
ATGATTCGGTTGTACCCGGAACAACTCCGCGCGCAGCTCAATGAAGGGCTGCGCGCGGCG
TATCTTTTACTTGGTAACGATCCTCTGTTATTGCAGGAAAGCCAGGACGCTGTTCGTCAG
GTAGCTGCGGCACAAGGATTCGAAGAACACCACACTTTTTCCATTGATCCCAACACTGAC
TGGAATGCGATCTTTTCGTTATGCCAGGCTATGAGTCTGTTTGCCAGTCGACAAACGCTA
TTGCTGTTGTTACCAGAAAACGGACCGAATGCGGCGATCAATGAGCAACTTCTCACACTC
ACCGGACTTCTGCATGACGACCTGCTGTTGATCGTCCGCGGTAATAAATTAAGCAAAGCG
CAAGAAAATGCCGCCTGGTTTACTGCGCTTGCGAATCGCAGCGTGCAGGTGACCTGTCAG
ACACCGGAGCAGGCTCAGCTTCCCCGCTGGGTTGCTGCGCGCGCAAAACAGCTCAACTTA
GAACTGGATGACGCGGCAAATCAGGTGCTCTGCTACTGTTATGAAGGTAACCTGCTGGCG
CTGGCTCAGGCACTGGAGCGTTTATCGCTGCTCTGGCCAGACGGCAAATTGACATTACCG
CGCGTTGAACAGGCGGTGAATGATGCCGCGCATTTCACCCCTTTTCATTGGGTTGATGCT
TTGTTGATGGGAAAAAGTAAGCGCGCATTGCATATTCTTCAGCAACTGCGTCTGGAAGGC
AGCGAACCGGTTATTTTGTTGCGCACATTACAACGTGAACTGTTGTTACTGGTTAACCTG
AAACGCCAGTCTGCCCATACGCCACTGCGTGCGTTGTTTGATAAGCATCGGGTATGGCAG
AACCGCCGGGGCATGATGGGCGAGGCGTTAAATCGCTTAAGTCAGACGCAGTTACGTCAG
GCCGTGCAACTCCTGACACGAACGGAACTCACCCTCAAACAAGATTACGGTCAGTCAGTG
TGGGCAGAGCTGGAAGGGTTATCTCTTCTGTTGTGCCATAAACCCCTGGCGGACGTATTT
ATCGACGGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:343
Protein Molecular Weight:38703
Protein Theoretical pI:7
PDB File:1XXI
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA polymerase III subunit delta
MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAAQGFEEHHTFSIDPNTD
WNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKA
QENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLA
LAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRLEG
SEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQTQLRQ
AVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHKPLADVFIDG
References
External Links:
ResourceLink
Uniprot ID:P28630
Uniprot Name:HOLA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651267
PDB ID:1XXI
Ecogene ID:EG11412
Ecocyc:EG11412
ColiBase:b0640
Kegg Gene:b0640
EchoBASE ID:EB1384
CCDB:HOLA_ECOLI
BacMap:16128623
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Carter, J. R., Franden, M. A., Aebersold, R., McHenry, C. S. (1992). "Molecular cloning, sequencing, and overexpression of the structural gene encoding the delta subunit of Escherichia coli DNA polymerase III holoenzyme." J Bacteriol 174:7013-7025. Pubmed: 1400251
  • Dong, Z., Onrust, R., Skangalis, M., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'." J Biol Chem 268:11758-11765. Pubmed: 8505303
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • O'Donnell, M. (1992). "Accessory protein function in the DNA polymerase III holoenzyme from E. coli." Bioessays 14:105-111. Pubmed: 1575709
  • Onrust, R., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. II. Characterization of delta and delta'." J Biol Chem 268:11766-11772. Pubmed: 8505304
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Takase, I., Ishino, F., Wachi, M., Kamata, H., Doi, M., Asoh, S., Matsuzawa, H., Ohta, T., Matsuhashi, M. (1987). "Genes encoding two lipoproteins in the leuS-dacA region of the Escherichia coli chromosome." J Bacteriol 169:5692-5699. Pubmed: 3316191