Soluble pyridine nucleotide transhydrogenase (P27306)

Identification
Name:Soluble pyridine nucleotide transhydrogenase
Synonyms:
  • STH
  • NAD(P)(+) transhydrogenase [B-specific]
Gene Name:sthA
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0NAD + 1.0NADPH ↔ 1.0NADH + 1.0NADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0NAD + 1.0NADPH ↔ 1.0NADH + 1.0NADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0NADPH + 1.0NAD → 1.0NADP + 1.0NADH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
binding
catalytic activity
FAD or FADH2 binding
nucleoside binding
oxidoreductase activity
purine nucleoside binding
Process
cell redox homeostasis
cellular homeostasis
cellular process
metabolic process
oxidation reduction
Gene Properties
Blattner:b3962
Gene OrientationCounterclockwise
Centisome Percentage:89.61
Left Sequence End4157413
Right Sequence End4158813
Gene Sequence:
>1401 bp
ATGGGGCAATCATCGCAACCACATGAGTTAGGCGGCGGGCTGAAGTCACGCCACGTCACC
ATGTTGTCTATTGCCGGTGTTATCGGCGCAAGTCTGTTTGTCGGTTCCAGCGTCGCCATC
GCCGAAGCGGGCCCGGCGGTATTACTGGCCTATCTGTTCGCCGGGCTACTGGTGGTTATG
ATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACC
TATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGG
TTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGG
GTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGT
AACTTATTAAGCGTTAAAAACTACGGCGAATTTGAGTTCTGGCTGGCGCTGTGCAAAGTC
ATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTAT
GCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTTTATGCCCAACGGTTTC
GGTGCGGTATTAAGCGCGATGTTGATCACCATGTTCTCGTTTATGGGCGCAGAAATTGTC
ACCATTGCCGCCGCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCG
GTTATCTGGCGTATTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATT
CCGTGGAATATGCCCGGACTAAAAGCCGTCGGTTCTTATCGCTCGGTACTGGAATTGCTC
AATATTCCCCATGCGAAATTAATCATGGACTGCGTGATATTACTTTCCGTAACCAGTTGC
CTGAACTCGGCGCTGTATACCGCGTCAAGGATGCTCTACTCCTTAAGTCGTCGCGGTGAT
GCTCCCGCGGTAATGGGTAAAATCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTC
TCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTT
AAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTT
TCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATG
TGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTG
ATGCTATTCCGTCCGGCGCAACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATAGGG
ATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACA
CCCGTTCATAATACGCGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:466
Protein Molecular Weight:51560
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Soluble pyridine nucleotide transhydrogenase
MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAV
SRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFV
DEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTHPRIYDSDSILSMHHEPRHVL
IYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEY
EKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQ
TAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALVKGEATAHLIEDIPTGIYTIPEISSVG
KTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILFHRETKEILGIHCFGERAAEI
IHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
References
External Links:
ResourceLink
Uniprot ID:P27306
Uniprot Name:STHA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1800049
Ecogene ID:EG11428
Ecocyc:EG11428
ColiBase:b3962
Kegg Gene:b3962
EchoBASE ID:EB1398
CCDB:STHA_ECOLI
BacMap:90111670
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Boonstra, B., French, C. E., Wainwright, I., Bruce, N. C. (1999). "The udhA gene of Escherichia coli encodes a soluble pyridine nucleotide transhydrogenase." J Bacteriol 181:1030-1034. Pubmed: 9922271
  • Gustafsson, C., Warne, S. R. (1992). "Physical map of the oxyR-trmA region (minute 89.3) of the Escherichia coli chromosome." J Bacteriol 174:7878-7879. Pubmed: 1447162
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Tao, K., Makino, K., Yonei, S., Nakata, A., Shinagawa, H. (1989). "Molecular cloning and nucleotide sequencing of oxyR, the positive regulatory gene of a regulon for an adaptive response to oxidative stress in Escherichia coli: homologies between OxyR protein and a family of bacterial activator proteins." Mol Gen Genet 218:371-376. Pubmed: 2511419