Transketolase 1 (P27302)

Identification
Name:Transketolase 1
Synonyms:
  • TK 1
Gene Name:tktA
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Erythrose 4-phosphate + 1.0Xylulose 5-phosphate ↔ 1.0Fructose 6-phosphate + 1.0D-Glyceraldehyde 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Fructose 6-phosphate + 1.0D-Glyceraldehyde 3-phosphate ↔ 1.0D-Erythrose 4-phosphate + 1.0Xylulose 5-phosphate
ReactionCard
1.0Sedoheptulose 7-phosphate+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Sedoheptulose 7-phosphate + 1.0D-Glyceraldehyde 3-phosphate + 1.0D-Sedoheptulose 7-phosphate ↔ 1.0D-Ribose-5-phosphate + 1.0Xylulose 5-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0beta-D-Fructose 6-phosphate + 1.0D-Glyceraldehyde 3-phosphate ↔ 1.0D-Erythrose 4-phosphate + 1.0Xylulose 5-phosphate
ReactionCard
1.0Aminofructose 6-phosphate+1.0Thumb ↔ 1.0Iminoerythrose 4-phosphate+1.0Sedoheptulose 7-phosphate
1.0Aminofructose 6-phosphate + 1.0D-Ribose-5-phosphate ↔ 1.0Iminoerythrose 4-phosphate + 1.0Sedoheptulose 7-phosphate
ReactionCard
SMPDB Reactions:
1.0Xylulose 5-phosphate+1.0Thumb+1.0Thumb ↔ 1.0D-Sedoheptulose 7-phosphate+1.0D-Glyceraldehyde 3-phosphate+1.0Thumb+1.0Thumb
1.0Xylulose 5-phosphate + 1.0D-Ribose-5-phosphate + 1.0Xylulose 5-phosphate ↔ 1.0D-Sedoheptulose 7-phosphate + 1.0D-Glyceraldehyde 3-phosphate + 1.0D-Sedoheptulose 7-phosphate + 1.0D-Glyceraldehyde 3-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Ribose-5-phosphate + 1.0Xylulose 5-phosphate ↔ 1.0D-Glyceraldehyde 3-phosphate + 1.0D-Sedoheptulose 7-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Erythrose 4-phosphate + 1.0Xylulose 5-phosphate ↔ 1.0Fructose 6-phosphate + 1.0D-Glyceraldehyde 3-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB03971beta-D-Fructose 6-phosphateMetaboCard
ECMDB02649D-Erythrose 4-phosphateMetaboCard
ECMDB01112D-Glyceraldehyde 3-phosphateMetaboCard
ECMDB02033D-Ribose-5-phosphateMetaboCard
ECMDB01068D-Sedoheptulose 7-phosphateMetaboCard
ECMDB00124Fructose 6-phosphateMetaboCard
ECMDB04178Xylulose 5-phosphateMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
transferase activity, transferring aldehyde or ketonic groups
transketolase activity
Process
metabolic process
Gene Properties
Blattner:b2935
Gene OrientationCounterclockwise
Centisome Percentage:66.33
Left Sequence End3077666
Right Sequence End3079657
Gene Sequence:
>1992 bp
ATGTCCTCACGTAAAGAGCTTGCCAATGCTATTCGTGCGCTGAGCATGGACGCAGTACAG
AAAGCCAAATCCGGTCACCCGGGTGCCCCTATGGGTATGGCTGACATTGCCGAAGTCCTG
TGGCGTGATTTCCTGAAACACAACCCGCAGAATCCGTCCTGGGCTGACCGTGACCGCTTC
GTGCTGTCCAACGGCCACGGCTCCATGCTGATCTACAGCCTGCTGCACCTCACCGGTTAC
GATCTGCCGATGGAAGAACTGAAAAACTTCCGTCAGCTGCACTCTAAAACTCCGGGTCAC
CCGGAAGTGGGTTACACCGCTGGTGTGGAAACCACCACCGGTCCGCTGGGTCAGGGTATT
GCCAACGCAGTCGGTATGGCGATTGCAGAAAAAACGCTGGCGGCGCAGTTTAACCGTCCG
GGCCACGACATTGTCGACCACTACACCTACGCCTTCATGGGCGACGGCTGCATGATGGAA
GGCATCTCCCACGAAGTTTGCTCTCTGGCGGGTACGCTGAAGCTGGGTAAACTGATTGCA
TTCTACGATGACAACGGTATTTCTATCGATGGTCACGTTGAAGGCTGGTTCACCGACGAC
ACCGCAATGCGTTTCGAAGCTTACGGCTGGCACGTTATTCGCGACATCGACGGTCATGAC
GCGGCATCTATCAAACGCGCAGTAGAAGAAGCGCGCGCAGTGACTGACAAACCTTCCCTG
CTGATGTGCAAAACCATCATCGGTTTCGGTTCCCCGAACAAAGCCGGTACCCACGACTCC
CACGGTGCGCCGCTGGGCGACGCTGAAATTGCCCTGACCCGCGAACAACTGGGCTGGAAA
TATGCGCCGTTCGAAATCCCGTCTGAAATCTATGCTCAGTGGGATGCGAAAGAAGCAGGC
CAGGCGAAAGAATCCGCATGGAACGAGAAATTCGCTGCTTACGCGAAAGCTTATCCGCAG
GAAGCCGCTGAATTTACCCGCCGTATGAAAGGCGAAATGCCGTCTGACTTCGACGCTAAA
GCGAAAGAGTTCATCGCTAAACTGCAGGCTAATCCGGCGAAAATCGCCAGCCGTAAAGCG
TCTCAGAATGCTATCGAAGCGTTCGGTCCGCTGTTGCCGGAATTCCTCGGCGGTTCTGCT
GACCTGGCGCCGTCTAACCTGACCCTGTGGTCTGGTTCTAAAGCAATCAACGAAGATGCT
GCGGGTAACTACATCCACTACGGTGTTCGCGAGTTCGGTATGACCGCGATTGCTAACGGT
ATCTCCCTGCACGGTGGCTTCCTGCCGTACACCTCCACCTTCCTGATGTTCGTGGAATAC
GCACGTAACGCCGTACGTATGGCTGCGCTGATGAAACAGCGTCAGGTGATGGTTTACACC
CACGACTCCATCGGTCTGGGCGAAGACGGCCCGACTCACCAGCCGGTTGAGCAGGTCGCT
TCTCTGCGCGTAACCCCGAACATGTCTACATGGCGTCCGTGTGACCAGGTTGAATCCGCG
GTCGCGTGGAAATACGGTGTTGAGCGTCAGGACGGCCCGACCGCACTGATCCTCTCCCGT
CAGAACCTGGCGCAGCAGGAACGAACTGAAGAGCAACTGGCAAACATCGCGCGCGGTGGT
TATGTGCTGAAAGACTGCGCCGGTCAGCCGGAACTGATTTTCATCGCTACCGGTTCAGAA
GTTGAACTGGCTGTTGCTGCCTACGAAAAACTGACTGCCGAAGGCGTGAAAGCGCGCGTG
GTGTCCATGCCGTCTACCGACGCATTTGACAAGCAGGATGCTGCTTACCGTGAATCCGTA
CTGCCGAAAGCGGTTACTGCACGCGTTGCTGTAGAAGCGGGTATTGCTGACTACTGGTAC
AAGTATGTTGGCCTGAACGGTGCTATCGTCGGTATGACCACCTTCGGTGAATCTGCTCCG
GCAGAGCTGCTGTTTGAAGAGTTCGGCTTCACTGTTGATAACGTTGTTGCGAAAGCAAAA
GAACTGCTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:663
Protein Molecular Weight:72211
Protein Theoretical pI:5
PDB File:1QGD
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Transketolase 1
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRF
VLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGI
ANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMMEGISHEVCSLAGTLKLGKLIA
FYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSL
LMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAG
QAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKA
SQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDAAGNYIHYGVREFGMTAIANG
ISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVA
SLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGG
YVLKDCAGQPELIFIATGSEVELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESV
LPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAK
ELL
References
External Links:
ResourceLink
Uniprot ID:P27302
Uniprot Name:TKT1_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675745
PDB ID:1QGD
Ecogene ID:EG11427
Ecocyc:EG11427
ColiBase:b2935
Kegg Gene:b2935
EchoBASE ID:EB1397
CCDB:TKT1_ECOLI
BacMap:49176286
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293
  • Sprenger, G. A. (1993). "Nucleotide sequence of the Escherichia coli K-12 transketolase (tkt) gene." Biochim Biophys Acta 1216:307-310. Pubmed: 8241274
  • Szumanski, M. B., Boyle, S. M. (1990). "Analysis and sequence of the speB gene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli." J Bacteriol 172:538-547. Pubmed: 2153656
  • Zhang, J., Sprung, R., Pei, J., Tan, X., Kim, S., Zhu, H., Liu, C. F., Grishin, N. V., Zhao, Y. (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8:215-225. Pubmed: 18723842