Identification
Name:Lipopolysaccharide core biosynthesis protein rfaS
Synonyms:Not Available
Gene Name:rfaS
Enzyme Class:Not Available
Biological Properties
General Function:Involved in protein binding
Specific Function:Specific function unknown
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0a lipopolysaccharide+1.0Thumb ? 1.0a rhamnosyl lipopolysaccharide+1.0Thumb
1.0a lipopolysaccharide + 1.0Deoxythymidine diphosphate-L-rhamnose ? 1.0a rhamnosyl lipopolysaccharide + 1.0dTDP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0inner core oligosaccharide lipid A (E coli)
Metabolites:
ECMDB IDNameView
ECMDB06354Deoxythymidine diphosphate-L-rhamnoseMetaboCard
ECMDB01274dTDPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21343Kdo-phospho-heptosyl-phospho-heptosyl-heptosyl-kdo2-lipidAMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b3629
Gene OrientationCounterclockwise
Centisome Percentage:81.95
Left Sequence End3802204
Right Sequence End3803139
Gene Sequence:
>936 bp
ATGGCCGCCGTTAACTTACGTCATATTGAAATTTTTCATGCGGTAATGACCGCCGGAAGC
CTGACTGAGGCGGCACACCTGCTACACACCTCACAGCCAACCGTCAGCCGCGAACTTGCG
CGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCT
ACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGC
ATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGC
CTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTCCTGCAACCCTTTCTGGCACGTTAT
CCCGATGTCAGCTTAAATATCGTGCCCCAGGAATCACCGCTACTTGAAGAGTGGCTCTCG
GCCCAGCGTCATGATTTAGGACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGT
ACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTA
AAAAAGGTATTAACGCCGGATGATTTTCAGGGTGAGAACTACATCAGCCTTTCCCGTACT
GACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATG
ATCGTAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATT
TCGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGG
TTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCA
GCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCT
CTTGACGCTATTTTGTCGTCAGCTACGACAGCATAA
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:311
Protein Molecular Weight:36730
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide core biosynthesis protein rfaS
MTIYFINWVADYELKMIQYLKKKYKIKNITTPKKYNWINKKISKIGMDNAWLGRLFIKHY
LNDIKKDDIIIINDSVVNKGINKQILKNINCHKVLLLRNTVGEDFILDNANYFDIIYDFE
HRFIGNEKIKAIEQFFPIGMDEIRNYSLSDKNNSQPICFFLGRDKGRLQIINELAERLTT
LGCKLDFNVVKDKTSSTTSKYLIEKQISYEENIRRTLNANIIVDITKENQSGWTLRILEA
LFFNKKLITNNINVFGSEIYSESRFFIIGHDDWDKLEYFINSSVKPMDYDSLYKFSPDKM
MSTIVSDFIDK
References
External Links:
ResourceLink
Uniprot ID:P27126
Uniprot Name:RFAS_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675655
Ecogene ID:EG11350
Ecocyc:EG11350
ColiBase:b3629
Kegg Gene:b3629
EchoBASE ID:EB1325
CCDB:RFAS_ECOLI
BacMap:16131500
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pradel, E., Parker, C. T., Schnaitman, C. A. (1992). "Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core." J Bacteriol 174:4736-4745. Pubmed: 1624461
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620