Identification
Name:Lipopolysaccharide core heptosyltransferase rfaQ
Synonyms:Not Available
Gene Name:rfaQ
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transferase activity, transferring glycosyl groups
Specific Function:Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Lipopolysaccharide biosynthesis ec00540
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB01341ADPMetaboCard
ECMDB24180ADP-L-glycero-beta-D-manno-heptoseMetaboCard
ECMDB20113ADP-L-Glycero-D-manno-heptoseMetaboCard
ECMDB24221glucosyl-(heptosyl)2-Kdo2-lipid A-phosphateMetaboCard
ECMDB24222glucosyl-(heptosyl)3-Kdo2-lipid A-phosphateMetaboCard
ECMDB20529Glucosyl-heptosyl2-KDO2-lipid A-phosphateMetaboCard
ECMDB20530Glucosyl-heptosyl3-KDO2-lipid A-phosphateMetaboCard
ECMDB21342Heptosyl-phospho-heptosyl-heptosyl-kdo2-lipidAMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21350Phospho-heptosyl-heptosyl-kdo2-lipidAMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
Process
metabolic process
Gene Properties
Blattner:b3632
Gene OrientationCounterclockwise
Centisome Percentage:82.01
Left Sequence End3805087
Right Sequence End3806121
Gene Sequence:
>1035 bp
ATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTG
GTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCA
TTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCG
CGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTA
GCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCT
ATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCA
GTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTA
TTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTT
ATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTT
GAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAA
ACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT
CGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGT
TCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAA
GCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAG
CCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATT
TTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATT
GTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGAT
CTCAATAAAGAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:344
Protein Molecular Weight:38730
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide core heptosyltransferase rfaQ
MRYHGDMLLTTPVISTLKQNYPDAKIDMLLYQDTIPILSENPEINALYGISNKGAGTFDK
IKNVLSLIKTLRANNYDLVINLTDQWMVALLVRCLPARMKISQLYGHRQHGIWKKSFTHL
APIHGTHIVERNLSVLEPLGITDFYTDTTMSYAEDCWKKMRRELDALGVKDHYVVIQPTA
RQIFKCWDNDKFSKVIDALQQRGYQVVLTCGPSADDLACVDEIARGCETKPITGLAGKTR
FPELGALIDHAVLFIGVDSAPGHIAAAVKTPVISLFGATDHVFWRPWTENIIQFWAGNYQ
KMPTRHELDRNKKYLSVIPAEDVIAATEKLLPEDAPSADRNAQL
References
External Links:
ResourceLink
Uniprot ID:P25742
Uniprot Name:RFAQ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742635
Ecogene ID:EG11341
Ecocyc:EG11341
ColiBase:b3632
Kegg Gene:b3632
EchoBASE ID:EB1317
CCDB:RFAQ_ECOLI
BacMap:16131503
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Clementz, T. (1992). "The gene coding for 3-deoxy-manno-octulosonic acid transferase and the rfaQ gene are transcribed from divergently arranged promoters in Escherichia coli." J Bacteriol 174:7750-7756. Pubmed: 1447141
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Parker, C. T., Pradel, E., Schnaitman, C. A. (1992). "Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12." J Bacteriol 174:930-934. Pubmed: 1732225
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620