Identification
Name:Lipopolysaccharide core biosynthesis protein rfaG
Synonyms:
  • Glucosyltransferase I
Gene Name:rfaG
Enzyme Class:Not Available
Biological Properties
General Function:Involved in biosynthetic process
Specific Function:Involved in the addition of the first glucose residue to the lipopolysaccharide core
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Lipopolysaccharide biosynthesis ec00540
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0inner core oligosaccharide lipid A (E coli)+1.0Thumb1.0glucosyl-inner core oligosaccharide lipid A+1.0Thumb+1.0Thumb
1.0inner core oligosaccharide lipid A (E coli) + 1.0UDP-Glucose → 1.0glucosyl-inner core oligosaccharide lipid A + 1.0Hydrogen ion + 1.0Uridine 5'-diphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24219(heptosyl)2-Kdo2-lipid AMetaboCard
ECMDB24220glucosyl-(heptosyl)2-Kdo2-lipid AMetaboCard
ECMDB20528Glucosyl-heptosyl2-KDO2-lipid AMetaboCard
ECMDB20527Heptosyl2-KDO2-lipid AMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04171UDP-GlucoseMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
GO Classification:
Process
biosynthetic process
metabolic process
Gene Properties
Blattner:b3631
Gene OrientationCounterclockwise
Centisome Percentage:81.99
Left Sequence End3803966
Right Sequence End3805090
Gene Sequence:
>1125 bp
ATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATA
AAGCGCGTATGGGTCTACATCACTGGTGTGACCGATCACCATCAGAACAGCCAGCCCCAG
TCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAAC
TGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCC
CCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGAT
CCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATG
CCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAA
GAAATTATCTGGAAAAGTGAACGGTTGAAAAAGTCACGGCGTGTATGGATTTTTACCACC
GGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCG
CAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCC
GCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGT
AATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCC
CCTTTTAGCGATCGTGCCGATCGCACCGTGGTTGCCGGGCAGAGTTTTGGTGGGCTTTCC
GCGCTGTATGCCGGACTGCACTGGCCTGAACGCTTTGGCTGTGTATTAAGCCAGTCAGGA
TCGTACTGGTGGCCGCATCGGGGCGGGCAGCAAGAGGGCGTGTTACTTGAAAAGCTAAAA
GCTGGTGAAGTTAGCGCCGAAGGTCTGCGCATTGTGCTGGAAGCGGGTATTCGCGAGCCG
ATGATCATGCGGGCCAATCAGGCGCTGTATGCGCAATTACACCCCATAAAAGAATCCATT
TTCTGGCGTCAGGTTGACGGCGGACATGATGCGCTTTGTTGGCGCGGTGGCTTGATGCAG
GGGCTAATCGACCTCTGGCAACCACTTTTCCATGACAGGAGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:374
Protein Molecular Weight:42284
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide core biosynthesis protein rfaG
MIVAFCLYKYFPFGGLQRDFMRIASTVAARGHHVRVYTQSWEGDCPKAFELIQVPVKSHT
NHGRNAEYYAWVQNHLKEHPADRVVGFNKMPGLDVYFAADVCYAEKVAQEKGFLYRLTSR
YRHYAAFERATFEQGKSTKLMMLTDKQIADFQKHYQTEPERFQILPPGIYPDRKYSEQIP
NSREIYRQKNGIKEQQNLLLQVGSDFGRKGVDRSIEALASLPESLRHNTLLFVVGQDKPR
KFEALAEKLGVRSNVHFFSGRNDVSELMAAADLLLHPAYQEAAGIVLLEAITAGLPVLTT
AVCGYAHYIADANCGTVIAEPFSQEQLNEVLRKALTQSPLRMAWAENARHYADTQDLYSL
PEKAADIITGGLDG
References
External Links:
ResourceLink
Uniprot ID:P25740
Uniprot Name:RFAG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674705
Ecogene ID:EG11339
Ecocyc:EG11339
ColiBase:b3631
Kegg Gene:b3631
EchoBASE ID:EB1315
CCDB:RFAG_ECOLI
BacMap:16131502
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Clementz, T. (1992). "The gene coding for 3-deoxy-manno-octulosonic acid transferase and the rfaQ gene are transcribed from divergently arranged promoters in Escherichia coli." J Bacteriol 174:7750-7756. Pubmed: 1447141
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Parker, C. T., Pradel, E., Schnaitman, C. A. (1992). "Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12." J Bacteriol 174:930-934. Pubmed: 1732225
  • Pilipcinec, E., Huisman, T. T., Willemsen, P. T., Appelmelk, B. J., de Graaf, F. K., Oudega, B. (1994). "Identification by Tn10 transposon mutagenesis of host factors involved in the biosynthesis of K99 fimbriae of Escherichia coli: effect of LPS core mutations." FEMS Microbiol Lett 123:201-206. Pubmed: 7988890
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620