Fructokinase (P23917)

Identification
Name:Fructokinase
Synonyms:
  • D-fructose kinase
  • Manno(fructo)kinase
Gene Name:mak
Enzyme Class:
Biological Properties
General Function:Transcription
Specific Function:Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0D-Fructose ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Fructose ↔ 1.0ADP + 1.0beta-D-Fructose 6-phosphate
ReactionCard
1.0Thumb+1.0beta-D-Fructose ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0beta-D-Fructose ↔ 1.0ADP + 1.0beta-D-Fructose 6-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Fructose ↔ 1.0ADP + 1.0Fructose 6-phosphate
ReactionCard
SMPDB Reactions:
1.0β-D-fructofuranose+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0β-D-fructofuranose + 1.0Adenosine triphosphate + 1.0D-Fructose → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0D-tagatofuranose 6-phosphate + 1.0ADP
ReactionCard
1.0D-Fructose+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0D-Fructose + 1.0Adenosine triphosphate + 1.0D-Fructose → 1.0D-tagatofuranose 6-phosphate + 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Fructose → 1.0ADP + 1.0Fructose 6-phosphate + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Fructose → 1.0ADP + 1.0Fructose 6-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB03971beta-D-Fructose 6-phosphateMetaboCard
ECMDB00660D-FructoseMetaboCard
ECMDB24205D-tagatofuranose 6-phosphateMetaboCard
ECMDB00124Fructose 6-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b0394
Gene OrientationClockwise
Centisome Percentage:8.82
Left Sequence End409368
Right Sequence End410276
Gene Sequence:
>909 bp
GTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCA
GGGGAGCAGTTGTACCGCCATCGTCTGCCCACGCCGCGTGATGATTACCGGCAGACTATT
GAAACGATCGCCACGTTGGTTGATATGGCGGAGCAGGCGACGGGGCAGCGCGGAACGGTA
GGTATGGGCATTCCTGGCTCAATTTCGCCTTACACCGGTGTGGTGAAGAATGCCAATTCA
ACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTG
CGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCG
GGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTC
AATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTA
CCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAA
CAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTG
AGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTA
GCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTG
AATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTTA
TATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCG
GTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCA
CAAGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:302
Protein Molecular Weight:32500
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Fructokinase
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTV
GMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAA
GAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPLPWMDEDELRYREEVPCYCGK
QGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWP
QE
References
External Links:
ResourceLink
Uniprot ID:P23917
Uniprot Name:MAK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674535
Ecogene ID:EG11288
Ecocyc:EG11288
ColiBase:b0394
Kegg Gene:b0394
EchoBASE ID:EB1265
CCDB:MAK_ECOLI
BacMap:90111128
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Miller, B. G., Raines, R. T. (2004). "Identifying latent enzyme activities: substrate ambiguity within modern bacterial sugar kinases." Biochemistry 43:6387-6392. Pubmed: 15157072
  • Reeder, T., Schleif, R. (1991). "Mapping, sequence, and apparent lack of function of araJ, a gene of the Escherichia coli arabinose regulon." J Bacteriol 173:7765-7771. Pubmed: 1744033
  • Ryder, L., Sharples, G. J., Lloyd, R. G. (1996). "Recombination-dependent growth in exonuclease-depleted recBC sbcBC strains of Escherichia coli K-12." Genetics 143:1101-1114. Pubmed: 8807285
  • Sproul, A. A., Lambourne, L. T., Jean-Jacques, D. J, Kornberg, H. L. (2001). "Genetic control of manno(fructo)kinase activity in Escherichia coli." Proc Natl Acad Sci U S A 98:15257-15259. Pubmed: 11742072