ECMDB

Identification

Name:

Fructokinase

Synonyms:

D-fructose kinase
Manno(fructo)kinase

Gene Name:

mak

Enzyme Class:

2.7.1.4

Biological Properties

General Function:

Transcription

Specific Function:

Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine

Cellular Location:

Not Available

SMPDB Pathways:

Starch and sucrose metabolism PW000941

KEGG Pathways:

Amino sugar and nucleotide sugar metabolism ec00520
Fructose and mannose metabolism ec00051
Metabolic pathways eco01100
Starch and sucrose metabolism ec00500

KEGG Reactions:

1.0

1.0D-Fructose

↔

1.0

1.0Adenosine triphosphate + 1.0D-Fructose ↔ 1.0ADP + 1.0beta-D-Fructose 6-phosphate
ReactionCard

1.0

1.0beta-D-Fructose

↔

1.0

1.0Adenosine triphosphate + 1.0beta-D-Fructose ↔ 1.0ADP + 1.0beta-D-Fructose 6-phosphate
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0D-Fructose ↔ 1.0ADP + 1.0Fructose 6-phosphate
ReactionCard

SMPDB Reactions:

1.0β-D-fructofuranose

1.0

→

1.0Adenosine diphosphate

1.0

1.0β-D-fructofuranose + 1.0Adenosine triphosphate + 1.0D-Fructose → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0D-tagatofuranose 6-phosphate + 1.0ADP
ReactionCard

1.0D-Fructose

1.0

→

1.0

1.0Adenosine diphosphate

1.0

1.0D-Fructose + 1.0Adenosine triphosphate + 1.0D-Fructose → 1.0D-tagatofuranose 6-phosphate + 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0D-Fructose → 1.0ADP + 1.0Fructose 6-phosphate + 1.0Hydrogen ion
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0D-Fructose → 1.0ADP + 1.0Fructose 6-phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB03971	beta-D-Fructose 6-phosphate	MetaboCard
ECMDB00660	D-Fructose	MetaboCard
ECMDB24205	D-tagatofuranose 6-phosphate	MetaboCard
ECMDB00124	Fructose 6-phosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard

GO Classification:

Not Available

Gene Properties

Blattner:

b0394

Gene Orientation

Clockwise

Centisome Percentage:

8.82

Left Sequence End

409368

Right Sequence End

410276

Gene Sequence:

>909 bp
GTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCA
GGGGAGCAGTTGTACCGCCATCGTCTGCCCACGCCGCGTGATGATTACCGGCAGACTATT
GAAACGATCGCCACGTTGGTTGATATGGCGGAGCAGGCGACGGGGCAGCGCGGAACGGTA
GGTATGGGCATTCCTGGCTCAATTTCGCCTTACACCGGTGTGGTGAAGAATGCCAATTCA
ACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTG
CGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCG
GGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTC
AATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTA
CCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAA
CAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTG
AGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTA
GCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTG
AATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTTA
TATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCG
GTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCA
CAAGAGTAA

Protein Properties

Pfam Domain Function:

ROK (PF00480 )

Protein Residues:

302

Protein Molecular Weight:

32500

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Fructokinase
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTV
GMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAA
GAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPLPWMDEDELRYREEVPCYCGK
QGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWP
QE

References

External Links:

Resource	Link
Uniprot ID:	P23917
Uniprot Name:	MAK_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674535
Ecogene ID:	EG11288
Ecocyc:	EG11288
ColiBase:	b0394
Kegg Gene:	b0394
EchoBASE ID:	EB1265
CCDB:	MAK_ECOLI
BacMap:	90111128

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Miller, B. G., Raines, R. T. (2004). "Identifying latent enzyme activities: substrate ambiguity within modern bacterial sugar kinases." Biochemistry 43:6387-6392. Pubmed: 15157072
Reeder, T., Schleif, R. (1991). "Mapping, sequence, and apparent lack of function of araJ, a gene of the Escherichia coli arabinose regulon." J Bacteriol 173:7765-7771. Pubmed: 1744033
Ryder, L., Sharples, G. J., Lloyd, R. G. (1996). "Recombination-dependent growth in exonuclease-depleted recBC sbcBC strains of Escherichia coli K-12." Genetics 143:1101-1114. Pubmed: 8807285
Sproul, A. A., Lambourne, L. T., Jean-Jacques, D. J, Kornberg, H. L. (2001). "Genetic control of manno(fructo)kinase activity in Escherichia coli." Proc Natl Acad Sci U S A 98:15257-15259. Pubmed: 11742072

Fructokinase (P23917)