Thymidine kinase (P23331)

Identification
Name:Thymidine kinase
Synonyms:Not Available
Gene Name:tdk
Enzyme Class:
Biological Properties
General Function:Involved in thymidine kinase activity
Specific Function:Phosphorylates both thymidine and deoxyuridine
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:
  • Pyrimidine metabolism PW000942
  • salvage pathways of pyrimidine deoxyribonucleotides PW002061
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Deoxyuridine → 1.0ADP + 1.0dUMP + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Thymidine ↔ 1.0ADP + 1.05-Thymidylic acid + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Thymidine ↔ 1.0ADP + 1.05-Thymidylic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Deoxyuridine ↔ 1.0ADP + 1.0dUMP
ReactionCard
1.05-Fluorodeoxyuridine+1.0Thumb ↔ 1.05-Fluorodeoxyuridine monophosphate+1.0Thumb
1.05-Fluorodeoxyuridine + 1.0Adenosine triphosphate ↔ 1.05-Fluorodeoxyuridine monophosphate + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Deoxyuridine → 1.0ADP + 1.0dUMP + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Thymidine ↔ 1.0ADP + 1.05-Thymidylic acid + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Thymidine + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.05-Thymidylic acid + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Thymidine → 1.0ADP + 1.05-Thymidylic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB012275-Thymidylic acidMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00012DeoxyuridineMetaboCard
ECMDB01409dUMPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00273ThymidineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
deoxynucleoside kinase activity
kinase activity
nucleobase, nucleoside, nucleotide kinase activity
nucleoside binding
nucleoside kinase activity
purine nucleoside binding
thymidine kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
DNA metabolic process
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b1238
Gene OrientationClockwise
Centisome Percentage:27.86
Left Sequence End1292750
Right Sequence End1293367
Gene Sequence:
>618 bp
ATGACAACCCAGTATGGATTTTTTATTGATTCCAGCCGTTGCACCGGTTGCAAAACCTGC
GAGCTGGCCTGTAAAGACTACAAAGATTTGACGCCAGAAGTCAGCTTCCGCCGCATTTAT
GAATATGCTGGCGGCGACTGGCAGGAAGATAACGGTGTCTGGCACCAGAACGTGTTTGCC
TACTATCTGTCGATTTCATGTAACCACTGCGAAGATCCGGCTTGTACTAAAGTCTGCCCG
AGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATT
GGCTGCCGCTACTGCCATATGGCTTGCCCGTATGGCGCACCGCAATATAACGAAACGAAA
GGCCATATGACCAAATGCGATGGTTGTTATGACCGTGTTGCGGAGGGTAAAAAGCCGATC
TGTGTTGAATCTTGTCCGCTGCGGGCGCTGGATTTCGGCCCTATCGACGAGCTGCGTAAA
AAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAAT
ATTGTGATCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCA
AACCCGAAGGAGGTGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:205
Protein Molecular Weight:23456
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Thymidine kinase
MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRFGAGKVSSRIGLSSPAK
LFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSEVVDQLDIPVLCYGLRTDF
RGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQAGRPYNEGEQVVIGGNERYVS
VCRKHYKEALQVDSLTAIQERHRHD
References
External Links:
ResourceLink
Uniprot ID:P23331
Uniprot Name:KITH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651422
Ecogene ID:EG10994
Ecocyc:EG10994
ColiBase:b1238
Kegg Gene:b1238
EchoBASE ID:EB0987
CCDB:KITH_ECOLI
BacMap:16129199
General Reference:
  • Black, M. E., Hruby, D. E. (1991). "Nucleotide sequence of the Escherichia coli thymidine kinase gene provides evidence for conservation of functional domains and quaternary structure." Mol Microbiol 5:373-379. Pubmed: 2041474
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bockamp, E. O., Blasco, R., Vinuela, E. (1991). "Escherichia coli thymidine kinase: nucleotide sequence of the gene and relationships to other thymidine kinases." Gene 101:9-14. Pubmed: 2060797
  • Danchin, A., Krin, E. (1995). "Filling the gap between hns and adhE in Escherichia coli K12." Microbiology 141 ( Pt 4):959-960. Pubmed: 7773397
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232