ECMDB

Identification

Name:

Proton glutamate symport protein

Synonyms:

Glutamate-aspartate carrier protein

Gene Name:

gltP

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in sodium:dicarboxylate symporter activity

Specific Function:

This carrier protein is part of the Na(+)-independent, binding-protein-independent glutamate-aspartate transport system

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

D-Glutamine and D-glutamate metabolism PW000769
Glutathione metabolism PW000833
L-glutamate metabolism PW000789
Lysine biosynthesis PW000771
Porphyrin metabolism PW000936
glutathione metabolism II PW001927
glutathione metabolism III PW002018
peptidoglycan biosynthesis I PW000906
peptidoglycan biosynthesis I 2 PW002062

KEGG Pathways:

D-Glutamine and D-glutamate metabolism ec00471
Glutathione metabolism ec00480
Lysine biosynthesis ec00300

Transports:

Transport References:

Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831

Metabolites:

ECMDB ID	Name	View
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00191	L-Aspartic acid	MetaboCard
ECMDB00148	L-Glutamate	MetaboCard

GO Classification:

Component
cell part
membrane
Function
carboxylic acid transmembrane transporter activity
dicarboxylic acid transmembrane transporter activity
organic acid transmembrane transporter activity
sodium:dicarboxylate symporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
carboxylic acid transport
dicarboxylic acid transport
establishment of localization
organic acid transport
transport

Gene Properties

Blattner:

b4077

Gene Orientation

Clockwise

Centisome Percentage:

92.52

Left Sequence End

4292504

Right Sequence End

4293817

Gene Sequence:

>1314 bp
ATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCG
TTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTAT
CGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTG
CGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTT
GCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCG
ACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGGGCCAATATTGTAGCGCGTTCT
TTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTG
TTTACTGGTGGGCTGGGTGCGCACTATGGTGCCGAACGTCTGGGCGCTACGGTGATCCCG
ATGTCTGGCGGGCAGACGGAAAAACAAGCGCAACTGATCCGTGATTTTCAACCAGATATG
ATCATGGTTACGCCATCTTATTGCCTTAACCTGATTGAAGAGCTGGAGCGGCAGTTGGGC
GGTGATGCCAGCGGTTGCTCGCTGCGGGTTGGAGTATTTGGTGCTGAGCCGTGGACACAG
GCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTG
TCAGAAGTGATGGGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACC
ATTTGGGAAGATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCC
GATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATT
CGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGC
ATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTT
CCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTG
GAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGT
AGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATT
AAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGT
TCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGA

Protein Properties

Pfam Domain Function:

SDF (PF00375 )

Protein Residues:

437

Protein Molecular Weight:

47159

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

24-46
66-84
97-115
150-168
172-191
211-229
243-261
283-301
322-340
352-370
379-398
406-419

Protein Sequence:

>Proton glutamate symport protein
MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPAGDIFIHLIKMIVVPIV
ISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITLANVFQPGAGVDMSQLATV
DISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEMLPIIFFSVLFGLGLSSLPAT
HREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVTVANFGFSSLWPLAKLVLLVHFA
ILFFALVVLGIVARLCGLSVWILIRILKDELILAYSTASSESVLPRIIEKMEAYGAPVSI
TSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGIDLSIWQEIILVLTLMVTSKGIAGVPGV
SFVVLLATLGSVGIPLEGLAFIAGVDRILDMARTALNVVGNALAVLVIAKWEHKFDRKKA
LAYEREVLGKFDKTADQ

References

External Links:

Resource	Link
Uniprot ID:	P21345
Uniprot Name:	GLTP_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674945
Ecogene ID:	EG10405
Ecocyc:	EG10405
ColiBase:	b4077
Kegg Gene:	b4077
EchoBASE ID:	EB0400
CCDB:	GLTP_ECOLI
BacMap:	16131903

General Reference:

Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Tolner, B., Poolman, B., Wallace, B., Konings, W. N. (1992). "Revised nucleotide sequence of the gltP gene, which encodes the proton-glutamate-aspartate transport protein of Escherichia coli K-12." J Bacteriol 174:2391-2393. Pubmed: 1551855
Wallace, B., Yang, Y. J., Hong, J. S., Lum, D. (1990). "Cloning and sequencing of a gene encoding a glutamate and aspartate carrier of Escherichia coli K-12." J Bacteriol 172:3214-3220. Pubmed: 1971622

Proton glutamate symport protein (P21345)