Glucose-1-phosphatase (P19926)

Identification
Name:Glucose-1-phosphatase
Synonyms:
  • G1Pase
Gene Name:agp
Enzyme Class:
Biological Properties
General Function:Involved in acid phosphatase activity
Specific Function:Absolutely required for the growth of E.coli in a high- phosphate medium containing G-1-P as the sole carbon source
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:
  • Glycolysis / Gluconeogenesis ec00010
  • Microbial metabolism in diverse environments ec01120
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glucose 1-phosphate + 1.0Water ↔ 1.0alpha-D-Glucose + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glucose 1-phosphate + 1.0Water ↔ 1.0D-Glucose + 1.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Glucose 1-phosphate + 1.0Water → 1.0D-Glucose + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Galactose 1-phosphate + 1.0Water → 1.0D-Galactose + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB03345alpha-D-GlucoseMetaboCard
ECMDB00143D-GalactoseMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB04011Galactose 1-phosphateMetaboCard
ECMDB01586Glucose 1-phosphateMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
acid phosphatase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
phosphatase activity
phosphoric ester hydrolase activity
Gene Properties
Blattner:b1002
Gene OrientationClockwise
Centisome Percentage:22.95
Left Sequence End1064808
Right Sequence End1066049
Gene Sequence:
>1242 bp
ATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCT
CAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCAT
AACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAA
TGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTG
TATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAA
TGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACC
GCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAA
AAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTC
AGTGAACAGGCGGTGGCGGCAATGGAGAAAGAGCTCAGCAAACTCCAGCTTACCGACAGC
TACCAGCTACTGGAAAAAATCGTTAACTATAAAGATTCCCCTGCCTGTAAAGAGAAACAA
CAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGT
GTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTAC
GAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAG
GTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCG
CGCAATGTTGCGAAACCGCTGGTCAGTTATATCGACAAAGCTCTGGTCACCGATCGCACC
AGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACG
GCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGC
AAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAA
TATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCT
GCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCG
ATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:413
Protein Molecular Weight:45683
Protein Theoretical pI:5
PDB File:1NT4
Signaling Regions:
  • 1-22
Transmembrane Regions:
  • None
Protein Sequence:
>Glucose-1-phosphatase
MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRAPLANNGSVLEQSTPNK
WPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECPPPYTVYAYANSLQRTVAT
AQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAFSEQAVAAMEKELSKLQLTDS
YQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQEPGVSGPLKVGNSLVDAFTLQYY
EGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTSPEVARNVAKPLVSYIDKALVTDRT
SAPKITVLVGHDSNIASLLTALDFKPYQLHDQNERTPIGGKIVFQRWHDSKANRDLMKIE
YVYQSAEQLRNADALTLQAPAQRVTLELSGCPIDADGFCPMDKFDSVLNEAVK
References
External Links:
ResourceLink
Uniprot ID:P19926
Uniprot Name:AGP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651498
PDB ID:1NT4
Ecogene ID:EG10033
Ecocyc:EG10033
ColiBase:b1002
Kegg Gene:b1002
EchoBASE ID:EB0032
CCDB:AGP_ECOLI
BacMap:16128968
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lee, D. C., Cottrill, M. A., Forsberg, C. W., Jia, Z. (2003). "Functional insights revealed by the crystal structures of Escherichia coli glucose-1-phosphatase." J Biol Chem 278:31412-31418. Pubmed: 12782623
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Pradel, E., Marck, C., Boquet, P. L. (1990). "Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase." J Bacteriol 172:802-807. Pubmed: 2153660