4'-phosphopantetheinyl transferase entD (P19925)

Identification
Name:4'-phosphopantetheinyl transferase entD
Synonyms:
  • Enterobactin synthase component D
  • Enterochelin synthase D
Gene Name:entD
Enzyme Class:Not Available
Biological Properties
General Function:Involved in magnesium ion binding
Specific Function:Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin
Cellular Location:Not Available
SMPDB Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides PW000760
KEGG Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides ec01053
SMPDB Reactions:
6.0Thumb+3.0L-Serine+3.0Thumb+3.0Thumb → 6.0Thumb+1.0enterobactin+6.0Pyrophosphate+3.0Thumb
6.0Adenosine triphosphate + 3.0L-Serine + 3.02,3-Dihydroxybenzoic acid + 3.0L-Serine → 6.0Adenosine monophosphate + 1.0enterobactin + 6.0Pyrophosphate + 3.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Adenosine triphosphate + 1.02,3-Dihydroxybenzoic acid → 1.0Pyrophosphate + 1.0(2,3-Dihydroxybenzoyl)adenylic acid
ReactionCard
EcoCyc Reactions:
3.0Thumb+3.0Thumb → 6.0Thumb+1.0Thumb+9.0Thumb
3.0(2,3-Dihydroxybenzoyl)adenylic acid + 3.0L-Seryl-AMP → 6.0Adenosine monophosphate + 1.0Enterochelin + 9.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04053(2,3-Dihydroxybenzoyl)adenylic acidMetaboCard
ECMDB241842,3-Dihydroxybenzoic acidMetaboCard
ECMDB00061Adenosine 3',5'-diphosphateMetaboCard
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB21212EnterochelinMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00187L-SerineMetaboCard
ECMDB20608L-Seryl-AMPMetaboCard
GO Classification:
Component
enterobactin synthetase complex
macromolecular complex
protein complex
Function
binding
catalytic activity
cation binding
holo-[acyl-carrier-protein] synthase activity
ion binding
magnesium ion binding
metal ion binding
phosphotransferase activity, for other substituted phosphate groups
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
cellular aromatic compound metabolic process
cellular metabolic process
enterobactin biosynthetic process
enterobactin metabolic process
macromolecule biosynthetic process
metabolic process
Gene Properties
Blattner:b0583
Gene OrientationCounterclockwise
Centisome Percentage:13.12
Left Sequence End608682
Right Sequence End609302
Gene Sequence:
>630 bp
ATGGTCGATATGAAAACTACGCATACCTCCCTCCCCTTTGCCGGACATACGCTGCATTTT
GTTGAGTTCGATCCGGCGAATTTTTGTGAGCAGGATTTACTCTGGCTGCCGCACTACGCA
CAACTGCAACACGCTGGACGTAAACGTAAAACAGAGCATTTAGCCGGACGGATCGCTGCT
GTTTATGCTTTGCGGGAATATGGCTATAAATGTGTGCCCGCAATCGGCGAGCTACGCCAA
CCTGTCTGGCCTGCGGAGGTATACGGCAGTATTAGCCACTGTGGGACTACGGCATTAGCC
GTGGTATCTCGTCAACCGATTGGCATTGATATAGAAGAAATTTTTTCTGTACAAACCGCA
AGAGAATTGACAGACAACATTATTACACCAGCGGAACACGAGCGACTCGCAGACTGCGGT
TTAGCCTTTTCTCTGGCGCTGACACTGGCATTTTCCGCCAAAGAGAGCGCATTTAAGGCA
AGTGAGATCCAAACTGATGCAGGTTTTCTGGACTATCAGATAATTAGCTGGAATAAACAG
CAGGTCATCATTCATCGTGAGAATGAGATGTTTGCTGTGCACTGGCAGATAAAAGAAAAG
ATAGTCATAACGCTGTGCCAACACGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:209
Protein Molecular Weight:23604
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>4'-phosphopantetheinyl transferase entD
MVDMKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGRKRKTEHLAGRIAA
VYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQPIGIDIEEIFSVQTA
RELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEIQTDAGFLDYQIISWNKQ
QVIIHRENEMFAVHWQIKEKIVITLCQHD
References
External Links:
ResourceLink
Uniprot ID:P19925
Uniprot Name:ENTD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674704
Ecogene ID:EG10262
Ecocyc:EG10262
ColiBase:b0583
Kegg Gene:b0583
EchoBASE ID:EB0258
CCDB:ENTD_ECOLI
BacMap:16128566
General Reference:
  • Armstrong, S. K., Pettis, G. S., Forrester, L. J., McIntosh, M. A. (1989). "The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product." Mol Microbiol 3:757-766. Pubmed: 2526281
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Coderre, P. E., Earhart, C. F. (1989). "The entD gene of the Escherichia coli K12 enterobactin gene cluster." J Gen Microbiol 135:3043-3055. Pubmed: 2533240
  • Gehring, A. M., Bradley, K. A., Walsh, C. T. (1997). "Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate." Biochemistry 36:8495-8503. Pubmed: 9214294
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lambalot, R. H., Gehring, A. M., Flugel, R. S., Zuber, P., LaCelle, M., Marahiel, M. A., Reid, R., Khosla, C., Walsh, C. T. (1996). "A new enzyme superfamily - the phosphopantetheinyl transferases." Chem Biol 3:923-936. Pubmed: 8939709
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232