Glycogen debranching enzyme (P15067)

Identification
Name:Glycogen debranching enzyme
Synonyms:
  • Glycogen operon protein glgX
Gene Name:glgX
Enzyme Class:Not Available
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen
Cellular Location:Cytoplasmic
SMPDB Pathways:
  • Secondary metabolites: Trehalose Biosynthesis and Metabolism PW000968
  • Starch and sucrose metabolism PW000941
KEGG Pathways:
KEGG Reactions:
1.0Starch ↔ 1.0Thumb
1.0Starch ↔ 1.0Trehalose
ReactionCard
SMPDB Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Glycogen ↔ 1.0α,α-trehalose
ReactionCard
1.0Dextrin+1.0Thumb+1.0Thumb → 1.0debranched limit dextrin+1.0Thumb
1.0Dextrin + 1.0Water + 1.0Dextrin → 1.0debranched limit dextrin + 1.0Maltotetraose
ReactionCard
EcoCyc Reactions:
1.0a limit dextrin+1.0Thumb → 1.0a debranched limit dextrin+1.0Thumb
1.0a limit dextrin + 1.0Water → 1.0a debranched limit dextrin + 1.0Maltotetraose
ReactionCard
Complex Reactions:
1.0branching glycogen → 1.0Thumb
1.0branching glycogen → 1.0Glycogen
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21651DextrinMetaboCard
ECMDB21341GlycogenMetaboCard
ECMDB01296MaltotetraoseMetaboCard
ECMDB00975TrehaloseMetaboCard
ECMDB00494WaterMetaboCard
ECMDB24256α,α-trehaloseMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
glycogen debranching enzyme activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
ion binding
Process
alcohol metabolic process
carbohydrate metabolic process
glucose metabolic process
glycogen catabolic process
glycogen metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
primary metabolic process
small molecule metabolic process
Gene Properties
Blattner:b3431
Gene OrientationCounterclockwise
Centisome Percentage:76.89
Left Sequence End3567369
Right Sequence End3569342
Gene Sequence:
>1974 bp
ATGACACAACTCGCCATTGGCAAACCCGCTCCCCTCGGCGCGCATTACGACGGTCAGGGC
GTCAACTTCACACTTTTCTCCGCTCATGCCGAGCGGGTAGAACTGTGTGTCTTTGACGCC
AATGGCCAGGAACATCGCTATGACTTGCCAGGGCACAGTGGCGACATTTGGCACGGTTAT
CTGCCGGATGCGCGCCCGGGTTTGCGTTATGGTTATCGCGTTCATGGCCCCTGGCAACCC
GCCGAGGGGCATCGCTTTAACCCGGCGAAGTTGTTGATTGATCCTTGCGCGCGGCAAATT
GACGGGGAGTTTAAAGATAACCCGCTGCTGCACGCCGGTCATAATGAACCTGACTATCGC
GACAACGCCGCCATTGCGCCGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGAT
GATGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGA
TTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGG
CATCCGGTGATGATCAACTATTTGAAACAATTGGGCATTACCGCGCTGGAACTGCTGCCA
GTGGCGCAGTTTGCCAGTGAACCACGTCTGCAACGCATGGGGCTAAGTAACTACTGGGGT
TACAACCCGGTGGCGATGTTTGCGCTGCATCCGGCGTATGCCTGCTCGCCAGAAACGGCG
CTGGATGAGTTTCGCGATGCAATCAAAGCACTGCATAAAGCGGGTATCGAAGTCATTCTT
GATATCGTGCTCAACCATAGTGCGGAACTGGACCTCGACGGCCCGTTATTCTCGCTGCGT
GGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACC
GGTTGCGGCAACACGCTCAATTTGAGTCATCCGGCGGTGGTGGATTATGCCAGCGCCTGC
CTGCGTTATTGGGTAGAAACCTGCCACGTCGATGGTTTCCGCTTTGATCTGGCGGCAGTC
ATGGGCCGTACGCCAGAGTTCCGTCAGGATGCGCCGTTGTTTACCGCTATCCAGAACTGC
CCGGTGCTCTCGCAGGTGAAGTTAATTGCTGAACCGTGGGATATCGCTCCTGGTGGTTAT
CAGGTGGGAAATTTCCCGCCGCTGTTTGCCGAGTGGAACGATCATTTCCGCGATGCTGCC
CGTCGTTTCTGGCTACATTATGATTTGCCTCTGGGGGCGTTTGCCGGGCGTTTTGCTGCC
TCCAGCGATGTTTTTAAACGTAATGGTCGTCTGCCGAGTGCCGCGATTAATCTCGTCACC
GCGCATGACGGTTTTACGCTTCGCGACTGCGTTTGCTTCAACCATAAACACAATGAAGCA
AACGGAGAAGAAAATCGCGACGGGACCAACAACAATTACAGTAACAATCATGGTAAAGAA
GGGTTAGGCGGTTCTCTTGACCTGGTTGAACGGCGGCGCGACAGCATTCACGCCCTGTTA
ACAACGTTGTTGCTCTCCCAGGGTACGCCGATGTTACTGGCCGGTGACGAACATGGTCAC
AGCCAGCATGGCAATAACAATGCCTACTGTCAGGATAACCAATTAACCTGGTTGGACTGG
TCGCAGGCAAGCAGTGGTTTAACCGCATTTACCGCCGCGTTAATCCATCTGCGCAAGCGC
ATTCCCGCTTTGGTGGAGAATCGCTGGTGGGAAGAAGGCGACGGCAATGTCCGTTGGCTA
AATCGATATGCTCAACCTTTAAGCACGGATGAGTGGCAAAACGGGCCGAAACAGCTGCAA
ATTCTGCTCTCGGATCGCTTTTTGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATT
GTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTG
ATTACGGCTGTCTGGCAGGGACCTGCACACGGATTGTGTGTGTTCCAGAGATGA
Protein Properties
Pfam Domain Function:
Protein Residues:657
Protein Molecular Weight:73576
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glycogen debranching enzyme
MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQEHRYDLPGHSGDIWHGY
LPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYR
DNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKGLTYLHPEIPVEIRGTYKALG
HPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNYWGYNPVAMFALHPAYACSPETA
LDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRGIDNRSYYWIREDGDYHNWT
GCGNTLNLSHPAVVDYASACLRYWVETCHVDGFRFDLAAVMGRTPEFRQDAPLFTAIQNC
PVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWNDHFRDAARRFWLHYDLPLGAFAGRFAA
SSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKE
GLGGSLDLVERRRDSIHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDW
SQASSGLTAFTAALIHLRKRIPALVENRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQ
ILLSDRFLIAINATLEVTEIVLPAGEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR
References
External Links:
ResourceLink
Uniprot ID:P15067
Uniprot Name:GLGX_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676611
Ecogene ID:EG10381
Ecocyc:EG10381
ColiBase:b3431
Kegg Gene:b3431
EchoBASE ID:EB0376
CCDB:GLGX_ECOLI
BacMap:16131305
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Romeo, T., Kumar, A., Preiss, J. (1988). "Analysis of the Escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes." Gene 70:363-376. Pubmed: 2975249
  • Yang, H., Liu, M. Y., Romeo, T. (1996). "Coordinate genetic regulation of glycogen catabolism and biosynthesis in Escherichia coli via the CsrA gene product." J Bacteriol 178:1012-1017. Pubmed: 8576033