Chorismate synthase (P12008)

Identification
Name:Chorismate synthase
Synonyms:
  • 5-enolpyruvylshikimate-3-phosphate phospholyase
Gene Name:aroC
Enzyme Class:
Biological Properties
General Function:Involved in chorismate synthase activity
Specific Function:5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Metabolic pathways eco01100
  • Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.05-O-(1-Carboxyvinyl)-3-phosphoshikimate ↔ 1.0Chorismate + 1.0Phosphate
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.05-O-(1-Carboxyvinyl)-3-phosphoshikimate → 1.0Phosphate + 1.0Chorismate
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.05-enolpyruvyl-shikimate 3-phosphate → 1.0Phosphate + 1.0Chorismate
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.05-O-(1-Carboxyvinyl)-3-phosphoshikimate ↔ 1.0Chorismate + 1.0Phosphate
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.05-O-(1-Carboxyvinyl)-3-phosphoshikimate → 1.0Phosphate + 1.0Chorismate
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.05-O-(1-Carboxyvinyl)-3-phosphoshikimate → 1.0Chorismate + 1.0Inorganic phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB242035-enolpyruvyl-shikimate 3-phosphateMetaboCard
ECMDB201065-O-(1-Carboxyvinyl)-3-phosphoshikimateMetaboCard
ECMDB12199ChorismateMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
GO Classification:
Function
carbon-oxygen lyase activity
carbon-oxygen lyase activity, acting on phosphates
catalytic activity
chorismate synthase activity
lyase activity
Process
aromatic amino acid family biosynthetic process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b2329
Gene OrientationCounterclockwise
Centisome Percentage:52.68
Left Sequence End2444410
Right Sequence End2445495
Gene Sequence:
>1086 bp
ATGGAAAGTGGTCATCGCTTTGATGCTCAGACGCTGCACAGTTTTATTCAGGCTGTATTT
CGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCA
AACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGG
AGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCG
GTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTG
GGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCAT
ATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCAC
TTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTT
GGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGAT
TACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCC
GTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATG
CAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCA
GCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACG
TTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAA
CTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCC
TCGCCGCATGATGATGATAAGCCGATTTTGCTACCGGGCGAGTGGGAAGTGAACACGCGT
CGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGAT
GCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCC
AGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:361
Protein Molecular Weight:39137
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Chorismate synthase
MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHDLDRRRPGTSRYTTQRR
EPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVFRPGHADYTYEQKYGLRDY
RGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQMGDIPLDIKDWSQVEQNPFFC
PDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGLGEPVFDRLDADIAHALMSINAV
KGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGILGGISSGQQIIAHMALKPTSSITV
PGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRQRAQNADVKTDIPR
W
References
External Links:
ResourceLink
Uniprot ID:P12008
Uniprot Name:AROC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062362
Ecogene ID:EG10075
Ecocyc:EG10075
ColiBase:b2329
Kegg Gene:b2329
EchoBASE ID:EB0073
CCDB:AROC_ECOLI
BacMap:16130264
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Charles, I. G., Lamb, H. K., Pickard, D., Dougan, G., Hawkins, A. R. (1990). "Isolation, characterization and nucleotide sequences of the aroC genes encoding chorismate synthase from Salmonella typhi and Escherichia coli." J Gen Microbiol 136:353-358. Pubmed: 2182772
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • White, P. J., Millar, G., Coggins, J. R. (1988). "The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa." Biochem J 251:313-322. Pubmed: 2969724
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837