Enterobactin synthase component F (P11454)

Identification
Name:Enterobactin synthase component F
Synonyms:
  • Enterochelin synthase F
  • Serine-activating enzyme
  • Seryl-AMP ligase
Gene Name:entF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in ligase activity
Specific Function:Activates the carboxylate group of L-serine via ATP- dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-entF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3- dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-entB (acylated with 2,3-dihydroxybenzoate by entE)
Cellular Location:Cytoplasmic
SMPDB Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides PW000760
  • Enterobactin Biosynthesis PW002048
KEGG Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides ec01053
SMPDB Reactions:
6.0Thumb+3.0L-Serine+3.0Thumb+3.0Thumb → 6.0Thumb+1.0enterobactin+6.0Pyrophosphate+3.0Thumb
6.0Adenosine triphosphate + 3.0L-Serine + 3.02,3-Dihydroxybenzoic acid + 3.0L-Serine → 6.0Adenosine monophosphate + 1.0enterobactin + 6.0Pyrophosphate + 3.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Adenosine triphosphate + 1.02,3-Dihydroxybenzoic acid → 1.0Pyrophosphate + 1.0(2,3-Dihydroxybenzoyl)adenylic acid
ReactionCard
1.0a 2,3-dihydroxybenzoyl-[EntB isochorismatase/aryl-carrier protein]+1.0a seryl-[EntF peptidyl-carrier protein] → 1.0a holo-[EntB isochorismatase/aryl-carrier protein]+1.0a DHB-seryl-[EntF peptidyl-carrier protein]+1.0Thumb
1.0a 2,3-dihydroxybenzoyl-[EntB isochorismatase/aryl-carrier protein] + 1.0a seryl-[EntF peptidyl-carrier protein] → 1.0a holo-[EntB isochorismatase/aryl-carrier protein] + 1.0a DHB-seryl-[EntF peptidyl-carrier protein] + 1.0Hydrogen ion
ReactionCard
3.0a DHB-seryl-[EntF peptidyl-carrier protein] → 1.0Thumb+3.0a holo-[EntF peptidyl-carrier protein]
3.0a DHB-seryl-[EntF peptidyl-carrier protein] → 1.0Enterochelin + 3.0a holo-[EntF peptidyl-carrier protein]
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0L-Serine ↔ 1.0Pyrophosphate + 1.0L-Seryl-AMP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Serine → 1.0Pyrophosphate + 1.0L-Seryl-AMP
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04053(2,3-Dihydroxybenzoyl)adenylic acidMetaboCard
ECMDB241842,3-Dihydroxybenzoic acidMetaboCard
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB21212EnterochelinMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00187L-SerineMetaboCard
ECMDB20608L-Seryl-AMPMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
acyl carrier activity
amino acid binding
binding
carboxylic acid binding
catalytic activity
cofactor binding
hydrolase activity
hydrolase activity, acting on ester bonds
ligase activity
phosphopantetheine binding
substrate-specific transporter activity
transporter activity
Process
biosynthetic process
metabolic process
Gene Properties
Blattner:b0586
Gene OrientationClockwise
Centisome Percentage:13.22
Left Sequence End613380
Right Sequence End617261
Gene Sequence:
>3882 bp
ATGAGCCAGCATTTACCTTTGGTCGCCGCACAGCCCGGCATCTGGATGGCAGAAAAACTG
TCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGAT
TCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATG
CGTTTTACGGAAGATAACGGCGAAGTCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAA
CTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTA
ATGCAGGCGGATTTGCAACAAGATCTGCGCGTCGATAGCGGTAAACCACTGGTCTTTCAT
CAGCTGATACAGGTGGCGGATAACCGCTGGTACTGGTATCAGCGTTATCACCATTTGCTG
GTCGATGGCTTTAGTTTCCCGGCCATTACCCGCCAGATCGCCAATATTTACTGCACATGG
CTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAG
TACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAG
CGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCC
TCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCT
ACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTGCGCTGGCAGCCTTGTGGCTG
GGGCGATTGTGCAATCGTATGGACTACGCCGCCGGATTTATCTTTATGCGTCGACTGGGC
TCGGCGGCGCTGACGGCTACCGGACCCGTGCTCAACGTTTTGCCGTTGGGTATTCACATT
GCGGCGCAAGAAACGCTGCCGGAACTGGCAACCCGACTGGCAGCACAACTGAAAAAAATG
CGTCGTCATCAACGTTACGATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGT
GATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATT
CCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTG
GCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGT
TACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTC
GCCGCGGATCCGGCGCTGTTGTGCGGCGATGTCGATATTATGCTGCCAGGTGAGTATGCG
CAGCTGGCGCAGCTCAACGCCACTCAGGTTGAGATTCCAGAAACCACGCTTAGCGCGCTG
GTGGCAGAACAAGCGGCAAAAACACCGGATGCTCCGGCGCTGGCAGATGCGCGTTACCTG
TTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGC
GGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTG
GCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCG
GACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTACCACCGAC
GATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCG
CTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACGGCTTATATC
ATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATC
GTCAACCGCCTGCTTTGGATGCAAAATCATTATCCGCTTACAGGCGAAGATGTCGTTGCC
CAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCA
GGGGCAAAACTGGTGATGGCTGAACCGGAAGCGCACCGCGACCCGCTCGCTATGCAGCAA
TTCTTTGCCGAATATGGCGTAACGACCACGCACTTTGTGCCGTCGATGCTGGCGGCATTT
GTTGCCTCGCTGACGCCGCAAACCGCTCGCCAGAGTTGCGCGACGTTGAAACAGGTTTTC
TGTAGTGGTGAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCG
CCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCAGCTGGTATCCGGCT
TTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGG
AATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGT
GATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACC
GCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGAC
GTTGCCCGCTGGCTGGATAACGGCGCGGTGGAGTACCTCGGGCGCAGTGATGATCAGCTA
AAAATTCGCGGGCAGCGTATCGAACTGGGCGAAATCGATCGCGTGATGCAGGCGCTGCCG
GATGTCGAACAAGCCGTTACCCACGCCTGTGTGATTAACCAGGCGGCTGCCACCGGTGGT
GATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGC
GCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTG
CAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTG
CCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATC
GCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTC
GCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTT
GCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACG
ATTATTGATGCTGAAGAAGACAGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTG
CGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAG
TTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGTCGATTATCGGCATTCAGTCACCG
CGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTG
GCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCTGGGGTATTCCCTTGGC
GGTACGCTGGCGCAGGGTATTGCGGCGCGACTGCGTGCCCGTGGCGAACAGGTGGCATTT
CTTGGCTTGCTGGATACCTGGCCGCCAGAAACGCAAAACTGGCAGGAAAAAGAAGCTAAT
GGTCTGGACCCGGAAGTGCTGGCGGAGATTAACCGCGAACGCGAGGCCTTCCTGGCAGCA
CAGCAGGGAAGTACTTCAACGGAGTTGTTTACCACCATTGAAGGCAACTACGCTGATGCT
GTGCGCCTGCTGACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTT
GCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCG
GAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTT
GAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:1293
Protein Molecular Weight:141990
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Enterobactin synthase component F
MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLLARAVVAGLAQADTLRM
RFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQADLQQDLRVDSGKPLVFH
QLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTWLRGEPTPASPFTPFADVVEE
YQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGRSASADILRLKLEFTDGEFRQLA
TQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFMRRLGSAALTATGPVLNVLPLGIHI
AAQETLPELATRLAAQLKKMRRHQRYDAEQIVRDSGRAAGDEPLFGPVLNIKVFDYQLDI
PDVQAQTHTLATGPVNDLELALFPDVHGDLSIEILANKQRYDEPTLIQHAERLKMLIAQF
AADPALLCGDVDIMLPGEYAQLAQLNATQVEIPETTLSALVAEQAAKTPDAPALADARYL
FSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYP
DDRLKMMLEDARPSLLITTDDQLPRFSDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYI
IFTSGSTGRPKGVMVGQTAIVNRLLWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIA
GAKLVMAEPEAHRDPLAMQQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVF
CSGEALPADLCREWQQLTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVW
NTGLRILDAMMHPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGD
VARWLDNGAVEYLGRSDDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGG
DARQLVGYLVSQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPL
PELKAQAPGRAPKAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQV
ARQVTPGQVMVASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQ
FSVLSRYLDPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLG
GTLAQGIAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAA
QQGSTSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIA
ELDIYRQDCAHVDIISPGTFEKIGPIIRATLNR
References
External Links:
ResourceLink
Uniprot ID:P11454
Uniprot Name:ENTF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674707
Ecogene ID:EG10264
Ecocyc:EG10264
ColiBase:b0586
Kegg Gene:b0586
EchoBASE ID:EB0260
CCDB:ENTF_ECOLI
BacMap:16128569
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gehring, A. M., Mori, I., Walsh, C. T. (1998). "Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF." Biochemistry 37:2648-2659. Pubmed: 9485415
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pettis, G. S., Brickman, T. J., McIntosh, M. A. (1988). "Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter." J Biol Chem 263:18857-18863. Pubmed: 2974033
  • Pettis, G. S., McIntosh, M. A. (1987). "Molecular characterization of the Escherichia coli enterobactin cistron entF and coupled expression of entF and the fes gene." J Bacteriol 169:4154-4162. Pubmed: 3040679
  • Rusnak, F., Sakaitani, M., Drueckhammer, D., Reichert, J., Walsh, C. T. (1991). "Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine." Biochemistry 30:2916-2927. Pubmed: 1826089