Isocitrate dehydrogenase kinase/phosphatase (P11071)

Identification
Name:Isocitrate dehydrogenase kinase/phosphatase
Synonyms:
  • IDH kinase/phosphatase
  • IDHK/P
Gene Name:aceK
Enzyme Class:
Biological Properties
General Function:Involved in [isocitrate dehydrogenase (NADP+)] kinase activity
Specific Function:Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0[Isocitrate dehydrogenase (NADP+)] ↔ 1.0Thumb+1.0[Isocitrate dehydrogenase (NADP+)] phosphate
1.0Adenosine triphosphate + 1.0[Isocitrate dehydrogenase (NADP+)] ↔ 1.0ADP + 1.0[Isocitrate dehydrogenase (NADP+)] phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0[isocitrate dehydrogenase (NADP(+))] → 1.0Thumb+1.0[isocitrate dehydrogenase (NADP(+))] phosphate
1.0Adenosine triphosphate + 1.0[isocitrate dehydrogenase (NADP(+))] → 1.0ADP + 1.0[isocitrate dehydrogenase (NADP(+))] phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
kinase activity
phosphatase activity
phosphoric ester hydrolase activity
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
[isocitrate dehydrogenase (NADP+)] kinase activity
Process
alcohol metabolic process
glucose metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Blattner:b4016
Gene OrientationClockwise
Centisome Percentage:90.88
Left Sequence End4216619
Right Sequence End4218355
Gene Sequence:
>1737 bp
ATGCCGCGTGGCCTGGAATTATTGATTGCTCAAACCATTTTGCAAGGCTTCGATGCTCAG
TATGGTCGATTCCTCGAAGTGACCTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGG
CATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTG
GTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTA
CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAG
AGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGG
CTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCG
AAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTA
CCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTG
ACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTG
TTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTG
CCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTG
ACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTAT
GCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACC
GCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAA
TATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGA
ATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGAC
AGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTG
AAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTG
GAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAA
ATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTG
CCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAA
TACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTT
AAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGC
TACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTT
GCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCAC
TGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAGAGATGCACGCCGACCTGTTC
CGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTT
TATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:578
Protein Molecular Weight:67698
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Isocitrate dehydrogenase kinase/phosphatase
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGL
VVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
LFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIIRHL
TETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
YLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDRFAPQKEMSAAHVRACYQLV
KEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIERRMV
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF
RADYWRALQNRIREGHVEDVYAYRRRQRFSVRYGEMLF
References
External Links:
ResourceLink
Uniprot ID:P11071
Uniprot Name:ACEK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676768
Ecogene ID:EG10026
Ecocyc:EG10026
ColiBase:b4016
Kegg Gene:b4016
EchoBASE ID:EB0025
CCDB:ACEK_ECOLI
BacMap:16131842
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cortay, J. C., Bleicher, F., Rieul, C., Reeves, H. C., Cozzone, A. J. (1988). "Nucleotide sequence and expression of the aceK gene coding for isocitrate dehydrogenase kinase/phosphatase in Escherichia coli." J Bacteriol 170:89-97. Pubmed: 2826408
  • Galinier, A., Bleicher, F., Negre, D., Perriere, G., Duclos, B., Cozzone, A. J., Cortay, J. C. (1991). "Primary structure of the intergenic region between aceK and iclR in the Escherichia coli chromosome." Gene 97:149-150. Pubmed: 1995429
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Klumpp, D. J., Plank, D. W., Bowdin, L. J., Stueland, C. S., Chung, T., LaPorte, D. C. (1988). "Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase." J Bacteriol 170:2763-2769. Pubmed: 2836370
  • Laporte, D. C., Stueland, C. S., Ikeda, T. P. (1989). "Isocitrate dehydrogenase kinase/phosphatase." Biochimie 71:1051-1057. Pubmed: 2557093
  • Matsuoka, M., McFadden, B. A. (1988). "Isolation, hyperexpression, and sequencing of the aceA gene encoding isocitrate lyase in Escherichia coli." J Bacteriol 170:4528-4536. Pubmed: 3049537
  • Oudot, C., Cortay, J. C., Blanchet, C., Laporte, D. C., Di Pietro, A., Cozzone, A. J., Jault, J. M. (2001). "The "catalytic" triad of isocitrate dehydrogenase kinase/phosphatase from E. coli and its relationship with that found in eukaryotic protein kinases." Biochemistry 40:3047-3055. Pubmed: 11258918