| Identification |
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| Name: | Lipid-A-disaccharide synthase |
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| Synonyms: | Not Available |
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| Gene Name: | lpxB |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Involved in lipid-A-disaccharide synthase activity |
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| Specific Function: | Condensation of UDP-2,3-diacylglucosamine and 2,3- diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | - Lipopolysaccharide biosynthesis PW000831
- lipopolysaccharide biosynthesis II PW001905
- lipopolysaccharide biosynthesis III PW002059
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| KEGG Pathways: | |
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| KEGG Reactions: | |
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| SMPDB Reactions: | |
1.0 | + | 1.0 | → | 1.0 | + | 1.0 | + | 1.0(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) |
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| EcoCyc Reactions: | |
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| Complex Reactions: | |
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| Metabolites: | | ECMDB ID | Name | View |
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| ECMDB20056 | 2,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate | MetaboCard | | ECMDB23240 | 2,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphate | MetaboCard | | ECMDB20054 | 2,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate | MetaboCard | | ECMDB23093 | 2,3-bis(3-Hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate | MetaboCard | | ECMDB24217 | 2,3-bis[(3R)-3-hydroxymyristoyl]-α-D-glucosaminyl 1-phosphate | MetaboCard | | ECMDB21225 | Hydrogen ion | MetaboCard | | ECMDB20201 | UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine | MetaboCard | | ECMDB24216 | UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine | MetaboCard | | ECMDB00295 | Uridine 5'-diphosphate | MetaboCard |
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| GO Classification: | | Function |
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| catalytic activity | | lipid-A-disaccharide synthase activity | | transferase activity | | transferase activity, transferring glycosyl groups | | UDP-glycosyltransferase activity | | Process |
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| lipid A biosynthetic process | | metabolic process | | organophosphate metabolic process | | phospholipid biosynthetic process | | phospholipid metabolic process |
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| Gene Properties |
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| Blattner: | b0182 |
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| Gene Orientation | Clockwise |
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| Centisome Percentage: | 4.38 |
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| Left Sequence End | 203348 |
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| Right Sequence End | 204496 |
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| Gene Sequence: | >1149 bp
TTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATA
GGGGCAACAGGTTCGGCGGTTGGGGAAGTCGTTTTCAATACTTCAATGACCGGTTATCAA
GAAATCCTCACTGATCCTTCCTATTCTCGTCAAATCGTTACTCTTACTTATCCCCATATT
GGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTG
GTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCT
TACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGACGCGT
TTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCG
GCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAA
GAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGC
CTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGT
GCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCG
GCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAAC
GGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAA
ACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGT
GCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTG
GAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACA
TTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATT
CATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCA
CACGACGCCGCGCCGTTGTTCGACCACTTTATCGAGTTAATTGAGCAGTACCGTAAAACC
GCTAAGTAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 382 |
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| Protein Molecular Weight: | 42382 |
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| Protein Theoretical pI: | 7 |
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| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >Lipid-A-disaccharide synthase
MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGPRMQAEGCEAWYEMEEL
AVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDFNITLEGNLKKQGIKTIHY
VSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVPCRFIGHTMADAMPLDPDKNA
ARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQLLRQTYPDLEIVVPLVNAKRREQ
FERIKAEVAPDLSVHLLDGMGREAMVASDAALLASGTAALECMLAKCPMVVGYRMKPFTF
WLAKRLVKTDYVSLPNLLAGRELVKELLQEECEPQKLAAALLPLLANGKTSHAMHDTFRE
LHQQIRCNADEQAAQAVLELAQ |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Coleman, J., Raetz, C. R. (1988). "First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene." J Bacteriol 170:1268-1274. Pubmed: 3277952
- Crowell, D. N., Reznikoff, W. S., Raetz, C. R. (1987). "Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase." J Bacteriol 169:5727-5734. Pubmed: 2824445
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Tomasiewicz, H. G., McHenry, C. S. (1987). "Sequence analysis of the Escherichia coli dnaE gene." J Bacteriol 169:5735-5744. Pubmed: 3316192
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