Lipid-A-disaccharide synthase (P10441)

Identification
Name:Lipid-A-disaccharide synthase
Synonyms:Not Available
Gene Name:lpxB
Enzyme Class:
Biological Properties
General Function:Involved in lipid-A-disaccharide synthase activity
Specific Function:Condensation of UDP-2,3-diacylglucosamine and 2,3- diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.02,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + 1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine ↔ 1.0Hydrogen ion + 1.02,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate + 1.0Uridine 5'-diphosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine + 1.02,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate ↔ 1.0Uridine 5'-diphosphate + 1.02,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.02,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + 1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine → 1.0Hydrogen ion + 1.02,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate + 1.0Uridine 5'-diphosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate)
1.02,3-bis[(3R)-3-hydroxymyristoyl]-α-D-glucosaminyl 1-phosphate + 1.0UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine → 1.0Uridine 5'-diphosphate + 1.0Hydrogen ion + 1.0(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate)
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.02,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + 1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine ↔ 1.0Hydrogen ion + 1.02,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate + 1.0Uridine 5'-diphosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.02,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + 1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine → 1.0Hydrogen ion + 1.02,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate + 1.0Uridine 5'-diphosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine + 1.02,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphate → 1.0Uridine 5'-diphosphate + 1.02,3-bis(3-Hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200562,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphateMetaboCard
ECMDB232402,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphateMetaboCard
ECMDB200542,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphateMetaboCard
ECMDB230932,3-bis(3-Hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphateMetaboCard
ECMDB242172,3-bis[(3R)-3-hydroxymyristoyl]-α-D-glucosaminyl 1-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB20201UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamineMetaboCard
ECMDB24216UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamineMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
GO Classification:
Function
catalytic activity
lipid-A-disaccharide synthase activity
transferase activity
transferase activity, transferring glycosyl groups
UDP-glycosyltransferase activity
Process
lipid A biosynthetic process
metabolic process
organophosphate metabolic process
phospholipid biosynthetic process
phospholipid metabolic process
Gene Properties
Blattner:b0182
Gene OrientationClockwise
Centisome Percentage:4.38
Left Sequence End203348
Right Sequence End204496
Gene Sequence:
>1149 bp
TTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATA
GGGGCAACAGGTTCGGCGGTTGGGGAAGTCGTTTTCAATACTTCAATGACCGGTTATCAA
GAAATCCTCACTGATCCTTCCTATTCTCGTCAAATCGTTACTCTTACTTATCCCCATATT
GGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTG
GTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCT
TACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGACGCGT
TTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCG
GCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAA
GAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGC
CTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGT
GCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCG
GCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAAC
GGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAA
ACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGT
GCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTG
GAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACA
TTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATT
CATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCA
CACGACGCCGCGCCGTTGTTCGACCACTTTATCGAGTTAATTGAGCAGTACCGTAAAACC
GCTAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:382
Protein Molecular Weight:42382
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipid-A-disaccharide synthase
MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGPRMQAEGCEAWYEMEEL
AVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDFNITLEGNLKKQGIKTIHY
VSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVPCRFIGHTMADAMPLDPDKNA
ARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQLLRQTYPDLEIVVPLVNAKRREQ
FERIKAEVAPDLSVHLLDGMGREAMVASDAALLASGTAALECMLAKCPMVVGYRMKPFTF
WLAKRLVKTDYVSLPNLLAGRELVKELLQEECEPQKLAAALLPLLANGKTSHAMHDTFRE
LHQQIRCNADEQAAQAVLELAQ
References
External Links:
ResourceLink
Uniprot ID:P10441
Uniprot Name:LPXB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321914
Ecogene ID:EG10546
Ecocyc:EG10546
ColiBase:b0182
Kegg Gene:b0182
EchoBASE ID:EB0541
CCDB:LPXB_ECOLI
BacMap:16128175
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Coleman, J., Raetz, C. R. (1988). "First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene." J Bacteriol 170:1268-1274. Pubmed: 3277952
  • Crowell, D. N., Reznikoff, W. S., Raetz, C. R. (1987). "Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase." J Bacteriol 169:5727-5734. Pubmed: 2824445
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Tomasiewicz, H. G., McHenry, C. S. (1987). "Sequence analysis of the Escherichia coli dnaE gene." J Bacteriol 169:5735-5744. Pubmed: 3316192