Phosphate-binding protein pstS (P0AG82)

Identification
Name:Phosphate-binding protein pstS
Synonyms:
  • PBP
Gene Name:pstS
Enzyme Class:Not Available
Biological Properties
General Function:Involved in phosphate ion binding
Specific Function:Part of the ABC transporter complex pstSACB involved in phosphate import
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+2.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Phosphate → 1.0ADP + 1.0Hydrogen ion + 2.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
anion transmembrane transporter activity
inorganic anion transmembrane transporter activity
inorganic phosphate transmembrane transporter activity
ion transmembrane transporter activity
phosphate transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
inorganic anion transport
ion transport
phosphate transport
transport
Gene Properties
Blattner:b3728
Gene OrientationCounterclockwise
Centisome Percentage:84.24
Left Sequence End3908508
Right Sequence End3909548
Gene Sequence:
>1041 bp
ATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTG
CTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTG
CAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGC
CCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAG
GTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGCGCG
GCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTA
AAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCTCAGGCGCAG
CGCCTCGCGAACGCCGGGCTGGATTACTACAACCACAACCTGGACACCTCGCCGGAGTTT
TACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTG
CGCGATGCCGGGATCAAAGTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAA
GATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCA
ATCAACATGCTGGTGAAGGTGAAAGGCACGCCGCTTGCCGATAACGATGATGTCGATGCC
TTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGC
CTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGC
GCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAA
GACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGG
GATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATAT
TACAACGCGGCAGCATTATGA
Protein Properties
Pfam Domain Function:
Protein Residues:346
Protein Molecular Weight:37024
Protein Theoretical pI:9
PDB File:1IXH
Signaling Regions:
  • 1-25
Transmembrane Regions:
  • None
Protein Sequence:
>Phosphate-binding protein pstS
MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQG
IGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIGGVVLAVNIPGLKSGELVL
DGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEE
WKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGK
PVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWPITSTTFILIHKDQKKPEQGTEVLK
FFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWKTNIKDSSGKPLY
References
External Links:
ResourceLink
Uniprot ID:P0AG82
Uniprot Name:PSTS_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674758
PDB ID:1IXH
Ecogene ID:EG10734
Ecocyc:EG10734
ColiBase:b3728
Kegg Gene:b3728
EchoBASE ID:EB0727
CCDB:PSTS_ECOLI
BacMap:16131596
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hirshberg, M., Henrick, K., Haire, L. L., Vasisht, N., Brune, M., Corrie, J. E., Webb, M. R. (1998). "Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate." Biochemistry 37:10381-10385. Pubmed: 9671506
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Luecke, H., Quiocho, F. A. (1990). "High specificity of a phosphate transport protein determined by hydrogen bonds." Nature 347:402-406. Pubmed: 2215649
  • Magota, K., Otsuji, N., Miki, T., Horiuchi, T., Tsunasawa, S., Kondo, J., Sakiyama, F., Amemura, M., Morita, T., Shinagawa, H., et, a. l. .. (1984). "Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli." J Bacteriol 157:909-917. Pubmed: 6365894
  • Surin, B. P., Jans, D. A., Fimmel, A. L., Shaw, D. C., Cox, G. B., Rosenberg, H. (1984). "Structural gene for the phosphate-repressible phosphate-binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene." J Bacteriol 157:772-778. Pubmed: 6321434
  • Wang, Z., Luecke, H., Yao, N., Quiocho, F. A. (1997). "A low energy short hydrogen bond in very high resolution structures of protein receptor--phosphate complexes." Nat Struct Biol 4:519-522. Pubmed: 9228942