GTP pyrophosphokinase (P0AG20)

Identification
Name:GTP pyrophosphokinase
Synonyms:
  • (p)ppGpp synthase
  • ATP:GTP 3'-pyrophosphotransferase
  • ppGpp synthase I
Gene Name:relA
Enzyme Class:
Biological Properties
General Function:Involved in amino acid binding
Specific Function:In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Guanosine triphosphate ↔ 1.0Adenosine monophosphate + 1.0Guanosine 3'-diphosphate 5'-triphosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Guanosine triphosphate ↔ 1.0Adenosine monophosphate + 1.0Guanosine 3'-diphosphate 5'-triphosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Guanosine diphosphate → 1.0Adenosine monophosphate + 1.0Hydrogen ion + 1.0Guanosine 3',5'-bis(diphosphate)
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Guanosine triphosphate ↔ 1.0Adenosine monophosphate + 1.0Guanosine 3'-diphosphate 5'-triphosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Guanosine diphosphate → 1.0Adenosine monophosphate + 1.0Guanosine 3',5'-bis(diphosphate)
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Guanosine triphosphate + 1.0Adenosine triphosphate → 1.0Guanosine 3'-diphosphate 5'-triphosphate + 1.0Adenosine monophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB20158Guanosine 3',5'-bis(diphosphate)MetaboCard
ECMDB20156Guanosine 3'-diphosphate 5'-triphosphateMetaboCard
ECMDB01201Guanosine diphosphateMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Function
amino acid binding
binding
carboxylic acid binding
Process
cellular nitrogen compound metabolic process
guanosine metabolic process
guanosine tetraphosphate metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process
purine ribonucleoside metabolic process
ribonucleoside metabolic process
Gene Properties
Blattner:b2784
Gene OrientationCounterclockwise
Centisome Percentage:62.71
Left Sequence End2909439
Right Sequence End2911673
Gene Sequence:
>2235 bp
ATGGTTGCGGTAAGAAGTGCACATATCAATAAGGCTGGTGAATTTGATCCGGAAAAATGG
ATCGCAAGTCTGGGTATTACCAGCCAGAAGTCGTGTGAGTGCTTAGCCGAAACCTGGGCG
TATTGTCTGCAACAGACGCAGGGGCATCCGGATGCCAGTCTGTTATTGTGGCGTGGTGTT
GAGATGGTGGAGATCCTCTCGACATTAAGTATGGACATTGACACGCTGCGGGCGGCGCTG
CTTTTCCCTCTGGCGGATGCCAACGTAGTCAGCGAAGATGTGCTGCGTGAGAGCGTCGGT
AAGTCGGTCGTTAACCTTATTCACGGCGTGCGTGATATGGCGGCGATCCGCCAGCTGAAA
GCGACGCACACTGATTCTGTTTCCTCCGAACAGGTCGATAACGTTCGCCGGATGTTATTG
GCGATGGTCGATGATTTTCGCTGCGTAGTCATCAAACTGGCGGAGCGTATTGCTCATCTG
CGCGAAGTAAAAGATGCGCCGGAAGATGAACGTGTACTGGCGGCAAAAGAGTGTACCAAC
ATCTACGCACCGCTGGCTAACCGTCTCGGAATCGGACAACTGAAATGGGAACTGGAAGAT
TACTGCTTCCGTTACCTCCATCCAACCGAATACAAACGAATTGCCAAACTGCTGCATGAA
CGGCGTCTCGACCGCGAACACTACATCGAAGAGTTCGTTGGTCATCTGCGCGCTGAGATG
AAAGCTGAAGGCGTTAAAGCGGAAGTGTATGGTCGTCCGAAACACATCTACAGCATCTGG
CGTAAAATGCAGAAAAAGAACCTCGCCTTTGATGAGCTGTTTGATGTGCGTGCGGTACGT
ATTGTCGCCGAGCGTTTACAGGATTGCTATGCCGCACTGGGGATAGTGCACACTCACTAT
CGCCACCTGCCGGATGAGTTTGACGATTACGTCGCTAACCCGAAACCAAACGGTTATCAG
TCTATTCATACCGTGGTTCTGGGGCCGGGTGGAAAAACCGTTGAGATCCAAATCCGCACC
AAACAGATGCATGAAGATGCAGAGTTGGGTGTTGCTGCGCACTGGAAATATAAAGAGGGC
GCGGCTGCTGGCGGCGCACGTTCGGGACATGAAGACCGGATTGCCTGGCTGCGTAAACTG
ATTGCGTGGCAGGAAGAGATGGCTGATTCCGGCGAAATGCTCGACGAAGTACGTAGTCAG
GTCTTTGACGACCGGGTGTACGTCTTTACGCCGAAAGGTGATGTCGTTGATTTGCCTGCG
GGATCAACGCCGCTGGACTTCGCTTACCACATCCACAGTGATGTCGGACACCGCTGCATC
GGGGCAAAAATTGGCGGGCGCATTGTGCCGTTCACCTACCAGCTGCAGATGGGCGACCAG
ATTGAAATTATCACCCAGAAACAGCCGAACCCCAGCCGTGACTGGTTAAACCCAAACCTC
GGTTACGTCACAACCAGCCGTGGGCGTTCGAAAATTCACGCCTGGTTCCGTAAACAGGAC
CGTGACAAAAACATTCTGGCTGGGCGGCAAATCCTTGACGACGAGCTGGAACATCTGGGG
ATCAGCCTGAAAGAAGCAGAAAAACATCTGCTGCCGCGTTACAACTTCAATGATGTCGAC
GAGTTGCTGGCGGCGATTGGTGGCGGGGATATCCGTCTCAATCAGATGGTGAACTTCCTG
CAATCGCAATTTAATAAGCCGAGTGCCGAAGAGCAGGACGCCGCCGCGCTGAAGCAACTT
CAGCAAAAAAGCTACACGCCGCAAAACCGCAGTAAAGATAACGGTCGCGTGGTAGTCGAA
GGTGTTGGCAACCTGATGCACCACATCGCGCGCTGCTGCCAGCCGATTCCTGGAGATGAG
ATTGTCGGCTTCATTACCCAGGGGCGCGGTATTTCAGTACACCGCGCCGATTGCGAACAA
CTGGCGGAACTGCGCTCCCATGCGCCAGAACGCATTGTTGACGCGGTATGGGGTGAGAGC
TACTCCGCCGGATATTCGCTGGTGGTCCGCGTGGTAGCTAATGATCGTAGTGGGTTGTTA
CGTGATATCACGACCATTCTCGCCAACGAGAAGGTGAACGTGCTTGGCGTTGCCAGCCGT
AGCGACACCAAACAGCAACTGGCGACCATCGACATGACCATTGAGATTTACAACCTGCAA
GTGCTGGGGCGCGTGCTGGGTAAACTCAACCAGGTGCCGGATGTTATCGACGCGCGTCGG
TTGCACGGGAGTTAG
Protein Properties
Pfam Domain Function:
Protein Residues:744
Protein Molecular Weight:83875
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>GTP pyrophosphokinase
MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQQTQGHPDASLLLWRGV
EMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKSVVNLIHGVRDMAAIRQLK
ATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHLREVKDAPEDERVLAAKECTN
IYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLLHERRLDREHYIEEFVGHLRAEM
KAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDVRAVRIVAERLQDCYAALGIVHTHY
RHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTVEIQIRTKQMHEDAELGVAAHWKYKEG
AAAGGARSGHEDRIAWLRKLIAWQEEMADSGEMLDEVRSQVFDDRVYVFTPKGDVVDLPA
GSTPLDFAYHIHSDVGHRCIGAKIGGRIVPFTYQLQMGDQIEIITQKQPNPSRDWLNPNL
GYVTTSRGRSKIHAWFRKQDRDKNILAGRQILDDELEHLGISLKEAEKHLLPRYNFNDVD
ELLAAIGGGDIRLNQMVNFLQSQFNKPSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVE
GVGNLMHHIARCCQPIPGDEIVGFITQGRGISVHRADCEQLAELRSHAPERIVDAVWGES
YSAGYSLVVRVVANDRSGLLRDITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQ
VLGRVLGKLNQVPDVIDARRLHGS
References
External Links:
ResourceLink
Uniprot ID:P0AG20
Uniprot Name:RELA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675603
Ecogene ID:EG10835
Ecocyc:EG10835
ColiBase:b2784
Kegg Gene:b2784
EchoBASE ID:EB0828
CCDB:RELA_ECOLI
BacMap:16130691
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Boehm, A., Steiner, S., Zaehringer, F., Casanova, A., Hamburger, F., Ritz, D., Keck, W., Ackermann, M., Schirmer, T., Jenal, U. (2009). "Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress." Mol Microbiol 72:1500-1516. Pubmed: 19460094
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Metzger, S., Dror, I. B., Aizenman, E., Schreiber, G., Toone, M., Friesen, J. D., Cashel, M., Glaser, G. (1988). "The nucleotide sequence and characterization of the relA gene of Escherichia coli." J Biol Chem 263:15699-15704. Pubmed: 2844820