Putative sulfate transporter ychM (P0AFR2)

Identification
Name:Putative sulfate transporter ychM
Synonyms:Not Available
Gene Name:ychM
Enzyme Class:Not Available
Biological Properties
General Function:Involved in secondary active sulfate transmembrane transporter activity
Specific Function:Possible sulfate transporter
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB00595Hydrogen carbonateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01448SulfateMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
anion transmembrane transporter activity
inorganic anion transmembrane transporter activity
ion transmembrane transporter activity
secondary active sulfate transmembrane transporter activity
substrate-specific transmembrane transporter activity
sulfate transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
inorganic anion transport
ion transport
sulfate transport
transmembrane transport
transport
Gene Properties
Blattner:b1206
Gene OrientationCounterclockwise
Centisome Percentage:27.12
Left Sequence End1258347
Right Sequence End1260026
Gene Sequence:
>1680 bp
GTGAACAAAATATTTTCCTCACATGTGATGCCTTTCCGCGCTCTGATCGACGCTTGCTGG
AAAGAAAAATATACTGCCGCACGGTTTACCCGTGACCTGATTGCCGGGATAACCGTCGGG
ATTATTGCTATCCCGCTGGCGATGGCGTTGGCTATTGGTAGTGGTGTGGCACCCCAGTAC
GGTTTATATACCGCAGCTGTTGCGGGGATTGTCATTGCTCTGACGGGTGGGTCACGCTTT
AGCGTTTCCGGTCCGACTGCGGCATTTGTGGTAATTCTCTATCCCGTGTCGCAACAGTTT
GGACTGGCAGGACTGCTGGTTGCGACCTTGCTGTCGGGGATCTTTTTGATTCTGATGGGT
CTGGCACGCTTTGGTCGCCTGATTGAGTATATTCCGGTTTCCGTCACCTTAGGTTTCACC
TCGGGTATCGGGATCACCATCGGTACCATGCAGATTAAAGATTTTCTCGGTCTGCAAATG
GCCCATGTCCCGGAACATTATCTACAAAAAGTCGGCGCATTATTTATGGCGCTGCCGACC
ATTAATGTGGGTGATGCTGCCATTGGCATTGTGACGCTAGGTATTCTTGTTTTTTGGCCG
CGTCTGGGCATTCGTTTACCCGGTCACCTTCCGGCCTTGCTGGCTGGTTGCGCGGTGATG
GGGATTGTTAACCTGCTCGGCGGACATGTTGCTACCATCGGTTCGCAATTCCACTACGTC
CTGGCCGATGGTTCTCAGGGTAACGGTATTCCGCAACTGCTGCCGCAACTGGTGCTGCCG
TGGGATCTGCCTAATTCAGAATTCACGCTAACCTGGGATTCTATTCGCACACTGCTGCCT
GCGGCATTCTCAATGGCAATGCTCGGCGCAATCGAATCTCTGCTCTGCGCCGTGGTGCTG
GATGGTATGACCGGGACGAAACACAAGGCGAACAGCGAACTGGTTGGACAGGGACTGGGG
AATATTATCGCTCCGTTCTTTGGTGGTATTACCGCTACAGCTGCCATCGCGCGTTCTGCC
GCTAACGTCCGTGCCGGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTT
ATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCA
GCCCTGCTGTTGATGGTGGCGTGGAACATGAGTGAAGCGCACAAAGTGGTCGACTTGCTG
CGTCATGCGCCGAAAGATGACATCATCGTCATGCTGCTGTGCATGTCGCTGACCGTGTTG
TTTGATATGGTTATTGCCATCAGCGTGGGGATCGTGCTGGCATCGCTGCTGTTTATGCGT
CGTATCGCACGTATGACTCGCCTGGCACCGGTAGTCGTAGATGTTCCAGACGATGTCCTG
GTTCTGCGCGTTATTGGCCCGCTGTTTTTTGCTGCTGCTGAAGGCTTATTCACGGACCTG
GAGTCACGTCTTGAAGGCAAACGGATTGTGATTCTGAAGTGGGATGCCGTTCCGGTACTT
GATGCTGGTGGTCTTGATGCGTTCCAGCGTTTTGTGAAGCGTCTGCCCGAGGGATGTGAA
CTGCGCGTGTGCAACGTGGAATTCCAGCCACTGCGCACTATGGCTCGCGCTGGCATTCAA
CCGATCCCGGGACGCCTGGCGTTCTTCCCGAATCGTCGCGCGGCGATGGCGGATTTATAA
Protein Properties
Pfam Domain Function:
Protein Residues:559
Protein Molecular Weight:59428
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 33-53
  • 55-75
  • 102-122
  • 127-147
  • 178-198
  • 213-233
  • 279-299
  • 314-334
  • 351-371
  • 419-439
Protein Sequence:
>Putative sulfate transporter ychM
MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQY
GLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGLAGLLVATLLSGIFLILMG
LARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQMAHVPEHYLQKVGALFMALPT
INVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCAVMGIVNLLGGHVATIGSQFHYV
LADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVL
DGMTGTKHKANSELVGQGLGNIIAPFFGGITATAAIARSAANVRAGATSPISAVIHSILV
ILALLVLAPLLSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDIIVMLLCMSLTVL
FDMVIAISVGIVLASLLFMRRIARMTRLAPVVVDVPDDVLVLRVIGPLFFAAAEGLFTDL
ESRLEGKRIVILKWDAVPVLDAGGLDAFQRFVKRLPEGCELRVCNVEFQPLRTMARAGIQ
PIPGRLAFFPNRRAAMADL
References
External Links:
ResourceLink
Uniprot ID:P0AFR2
Uniprot Name:YCHM_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674877
Ecogene ID:EG12392
Ecocyc:EG12392
ColiBase:b1206
Kegg Gene:b1206
CCDB:YCHM_ECOLI
BacMap:90111235
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., Rudd, K. E., Koonin, E. V. (1994). "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Nucleic Acids Res 22:4756-4767. Pubmed: 7984428
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hove-Jensen, B., Harlow, K. W., King, C. J., Switzer, R. L. (1986). "Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene." J Biol Chem 261:6765-6771. Pubmed: 3009477
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Sandal, N. N., Marcker, K. A. (1994). "Similarities between a soybean nodulin, Neurospora crassa sulphate permease II and a putative human tumour suppressor." Trends Biochem Sci 19:19. Pubmed: 8140616