Histidine-binding periplasmic protein (P0AEU0)

Identification
Name:Histidine-binding periplasmic protein
Synonyms:
  • HBP
Gene Name:hisJ
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
SMPDB Reactions:
1.0L-Histidine+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0L-Histidine+1.0Thumb
1.0L-Histidine + 1.0Adenosine triphosphate + 1.0Water + 1.0L-Histidine → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0L-Histidine + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0L-Histidine → 1.0ADP + 1.0Hydrogen ion + 1.0L-Histidine + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00177L-HistidineMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Function
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b2309
Gene OrientationCounterclockwise
Centisome Percentage:52.25
Left Sequence End2424028
Right Sequence End2424810
Gene Sequence:
>783 bp
ATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGAC
GTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTG
TTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACC
ACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTA
CACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAG
GGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTC
CAGGTGGATCAGAACGGGCAGCTGGTGACGGCTGGTGGTTTTCAGGTGCAGCCAGCGATC
ACCATTCCGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACC
CAACAAGGCCAGGCAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAAT
GACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCA
CCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAA
ACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTAC
GAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTC
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:260
Protein Molecular Weight:28483
Protein Theoretical pI:5
PDB File:1HSL
Signaling Regions:
  • 1-22
Transmembrane Regions:
  • None
Protein Sequence:
>Histidine-binding periplasmic protein
MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNSQGELVGFDIDLAKELC
KRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQEIAFTDKLYAADSRLVVAK
NSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIVSYQGQDNIYSDLTAGRIDAA
FQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTGMGLRKEDNELREALNKAFAEMR
ADGTYEKLAKKYFDFDVYGG
References
External Links:
ResourceLink
Uniprot ID:P0AEU0
Uniprot Name:HISJ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062656
PDB ID:1HSL
Ecogene ID:EG12124
Ecocyc:EG12124
ColiBase:b2309
Kegg Gene:b2309
EchoBASE ID:EB2045
CCDB:HISJ_ECOLI
BacMap:16130244
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837
  • Yao, N., Trakhanov, S., Quiocho, F. A. (1994). "Refined 1.89-A structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport/chemosensory proteins." Biochemistry 33:4769-4779. Pubmed: 8161536