Glycolate oxidase subunit glcD (P0AEP9)

Identification
Name:Glycolate oxidase subunit glcD
Synonyms:Not Available
Gene Name:glcD
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Specific function unknown
Cellular Location:Not Available
SMPDB Pathways:
  • glycolate and glyoxylate degradation PW000827
KEGG Pathways:
  • Glyoxylate and dicarboxylate metabolism ec00630
  • Metabolic pathways eco01100
  • Microbial metabolism in diverse environments ec01120
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glycolic acid + 1.0Oxygen ↔ 1.0Glyoxylic acid + 1.0Hydrogen peroxide
ReactionCard
1.0(S)-2-Hydroxyacid+1.0Thumb ↔ 1.02-Oxo acid+1.0Thumb
1.0(S)-2-Hydroxyacid + 1.0Oxygen ↔ 1.02-Oxo acid + 1.0Hydrogen peroxide
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0an oxidized electron-transfer flavoprotein  → 1.0Reduced acceptor+1.0Thumb
1.0Glycolic acid + 1.0an oxidized electron-transfer flavoprotein  → 1.0Reduced acceptor + 1.0Glyoxylic acid
ReactionCard
2.0Thumb+2.0an oxidized electron-transfer flavoprotein  → 2.0Reduced acceptor+2.0Thumb
2.0Glycolic acid + 2.0an oxidized electron-transfer flavoprotein  → 2.0Reduced acceptor + 2.0Glyoxylic acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Glycolic acid + 1.0Ubiquinone-8 → 1.0Glyoxylic acid + 1.0Ubiquinol-8
ReactionCard
1.0Thumb+1.0Menaquinone 8 → 1.0Thumb+1.0Thumb
1.0Glycolic acid + 1.0Menaquinone 8 → 1.0Glyoxylic acid + 1.0Menaquinol 8
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Demethylmenaquinone 8 + 1.0Glycolic acid → 1.02-Demethylmenaquinol 8 + 1.0Glyoxylic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB23741GlycolateMetaboCard
ECMDB03035Glycolic acidMetaboCard
ECMDB00119Glyoxylic acidMetaboCard
ECMDB21232Hydrogen peroxideMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB04124OxygenMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Component
glycolate oxidase complex
macromolecular complex
protein complex
Function
(S)-2-hydroxy-acid oxidase activity
adenyl nucleotide binding
binding
catalytic activity
FAD or FADH2 binding
glycolate oxidase activity
nucleoside binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor
purine nucleoside binding
Gene Properties
Blattner:b2979
Gene OrientationCounterclockwise
Centisome Percentage:67.34
Left Sequence End3124544
Right Sequence End3126043
Gene Sequence:
>1500 bp
ATGACAGAGCCGCATGTAGCAGTATTAAGCCAGGTCCAACAGTTTCTCGATCGTCAACAC
GGTCTTTATATTGATGGTCGTCCTGGCCCCGCACAAAGTGAAAAACGGTTGGCGATCTTT
GATCCGGCCACCGGGCAAGAAATTGCGTCTACTGCTGATGCCAACGAAGCGGATGTAGAT
AACGCAGTCATGTCTGCCTGGCGGGCCTTTGTCTCGCGTCGCTGGGCCGGGCGATTACCC
GCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAG
CTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAA
GTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGT
AAAACGCTGGACTTGTCGATTCCCTTACCCCAGGGGGCGCGTTATCAGGCCTGGACGCGT
AAAGAGCCGGTTGGCGTAGTGGCGGGAATTGTGCCATGGAACTTTCCGTTGATGATTGGT
ATGTGGAAGGTGATGCCAGCACTGGCAGCAGGCTGTTCAATCGTGATTAAGCCTTCGGAA
ACCACGCCACTGACGATGTTGCGCGTGGCGGAACTGGCCAGCGAGGCTGGTATCCCTGAT
GGCGTTTTTAATGTCGTCACCGGGTCAGGTGCTGTATGCGGCGCGGCCCTGACGTCACAT
CCTCATGTTGCGAAAATCAGTTTTACCGGTTCAACCGCGACGGGAAAAGGTATTGCCAGA
ACTGCTGCTGATCACTTAACGCGTGTAACGCTGGAACTGGGCGGTAAAAACCCGGCAATT
GTATTAAAAGATGCTGATCCGCAATGGGTTATTGAAGGCTTGATGACCGGAAGCTTCCTG
AATCAAGGGCAAGTATGCGCCGCCAGTTCGCGAATTTATATTGAAGCGCCGTTGTTTGAC
ACGCTGGTTAGTGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCA
CCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCACTGCGACAAAGTGTGTTCATTC
CTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGGA
GAGGGGTATTATGTTGCGCCAACGCTGGTGGTAAATCCCGATGCTAAATTGCGCTTAACT
CGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCG
TTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGTCTGGACGCAAAATCTC
TCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCAT
ACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGAT
TTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:499
Protein Molecular Weight:53811
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glycolate oxidase subunit glcD
MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVV
LPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGR
RARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
KIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLAS
FDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVES
DVQEDCERVNDILLKAGATDVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPR
RALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELC
VEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAE
FGAMHVHHGHLPFPELERF
References
External Links:
ResourceLink
Uniprot ID:P0AEP9
Uniprot Name:GLCD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674936
Ecogene ID:EG12997
Ecocyc:EG12997
ColiBase:b2979
Kegg Gene:b2979
EchoBASE ID:EB2820
CCDB:GLCD_ECOLI
BacMap:16130879
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pellicer, M. T., Badia, J., Aguilar, J., Baldoma, L. (1996). "glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein." J Bacteriol 178:2051-2059. Pubmed: 8606183