UTP--glucose-1-phosphate uridylyltransferase_ (P0AEP3)

Identification
Name:UTP--glucose-1-phosphate uridylyltransferase_
Synonyms:
  • Alpha-D-glucosyl-1-phosphate uridylyltransferase
  • UDP-glucose pyrophosphorylase
  • UDPGP
  • Uridine diphosphoglucose pyrophosphorylase
Gene Name:galU
Enzyme Class:
Biological Properties
General Function:Involved in UTP:glucose-1-phosphate uridylyltransferase activity
Specific Function:May play a role in stationary phase survival
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glucose 1-phosphate + 1.0Hydrogen ion + 1.0Uridine triphosphate ↔ 1.0Pyrophosphate + 1.0UDP-Glucose
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Uridine triphosphate + 1.0Glucose 1-phosphate ↔ 1.0Pyrophosphate + 1.0UDP-Glucose
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Uridine triphosphate+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Glucose 1-phosphate + 1.0Uridine triphosphate + 1.0Hydrogen ion + 1.0Uridine triphosphate → 1.0Pyrophosphate + 1.0UDP-Glucose
ReactionCard
1.0Thumb+1.0Uridine triphosphate+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Pyrophosphate
1.0β-D-glucose 1-phosphate + 1.0Uridine triphosphate + 1.0Hydrogen ion + 1.0Uridine triphosphate → 1.0UDP-Glucose + 1.0Pyrophosphate
ReactionCard
1.0Alpha-D-glucose 1-phosphate+1.0Uridine triphosphate+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Alpha-D-glucose 1-phosphate + 1.0Uridine triphosphate + 1.0Hydrogen ion + 1.0Uridine triphosphate → 1.0Pyrophosphate + 1.0UDP-Glucose
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glucose 1-phosphate + 1.0Hydrogen ion + 1.0Uridine triphosphate ↔ 1.0Pyrophosphate + 1.0UDP-Glucose
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Glucose 1-phosphate + 1.0Uridine triphosphate → 1.0UDP-Glucose + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01586Glucose 1-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB04171UDP-GlucoseMetaboCard
ECMDB00285Uridine triphosphateMetaboCard
ECMDB24239β-D-glucose 1-phosphateMetaboCard
GO Classification:
Function
catalytic activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
uridylyltransferase activity
UTP-monosaccharide-1-phosphate uridylyltransferase activity
UTP:glucose-1-phosphate uridylyltransferase activity
Process
alcohol metabolic process
biosynthetic process
glucose metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
UDP-glucose metabolic process
Gene Properties
Blattner:b1236
Gene OrientationClockwise
Centisome Percentage:27.82
Left Sequence End1290680
Right Sequence End1291588
Gene Sequence:
>909 bp
GTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCA
GGGGAGCAGTTGTACCGCCATCGTCTGCCCACGCCGCGTGATGATTACCGGCAGACTATT
GAAACGATCGCCACGTTGGTTGATATGGCGGAGCAGGCGACGGGGCAGCGCGGAACGGTA
GGTATGGGCATTCCTGGCTCAATTTCGCCTTACACCGGTGTGGTGAAGAATGCCAATTCA
ACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTG
CGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCG
GGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTC
AATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTA
CCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAA
CAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTG
AGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTA
GCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTG
AATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTTA
TATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCG
GTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCA
CAAGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:302
Protein Molecular Weight:32942
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>UTP--glucose-1-phosphate uridylyltransferase
MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQYVVNECIAAGITEIVLV
THSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHVTIMQVRQGLAKGLGHAVL
CAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFDETGHSQIMVEPVADVTAYGV
VDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRYVLSADIWPLLAKTPPGAGDEIQ
LTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAFVEYGIRHNTLGTEFKAWLEEEMGI
KK
References
External Links:
ResourceLink
Uniprot ID:P0AEP3
Uniprot Name:GALU_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674535
Ecogene ID:EG11319
Ecocyc:EG11319
ColiBase:b1236
Kegg Gene:b1236
EchoBASE ID:EB1295
CCDB:GALU_ECOLI
BacMap:16129197
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hossain, S. A., Tanizawa, K., Kazuta, Y., Fukui, T. (1994). "Overproduction and characterization of recombinant UDP-glucose pyrophosphorylase from Escherichia coli K-12." J Biochem 115:965-972. Pubmed: 7961613
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Ueguchi, C., Ito, K. (1992). "Multicopy suppression: an approach to understanding intracellular functioning of the protein export system." J Bacteriol 174:1454-1461. Pubmed: 1537791
  • Weissborn, A. C., Liu, Q., Rumley, M. K., Kennedy, E. P. (1994). "UTP: alpha-D-glucose-1-phosphate uridylyltransferase of Escherichia coli: isolation and DNA sequence of the galU gene and purification of the enzyme." J Bacteriol 176:2611-2618. Pubmed: 8169209