ECMDB: Phosphatase nudJ (P0AEI6)

Identification

Name:

Phosphatase nudJ

Synonyms:

Not Available

Gene Name:

nudJ

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in hydrolase activity

Specific Function:

Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P), and hydrolysis of thiamine pyrophosphate (TPP) to thiamine monophosphate (TMP). Can hydrolyze other substrates such as MeO-HMP-PP, CF(3)-HMP-PP and MeO-TPP. Is also a non-specific nucleoside tri- and diphosphatase that releases inorganic orthophosphate

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

EcoCyc Reactions:

1.0

→

1.0

1.02-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 1.0Water → 1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard

1.0

→

1.0

1.0Water + 1.0Thiamine pyrophosphate → 1.0Hydrogen ion + 1.0Phosphate + 1.0Thiamine monophosphate
ReactionCard

1.0

→

1.0

1.0Guanosine diphosphate + 1.0Water → 1.0Hydrogen ion + 1.0Guanosine monophosphate + 1.0Phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB20046	2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate	MetaboCard
ECMDB20084	4-Amino-2-methyl-5-phosphomethylpyrimidine	MetaboCard
ECMDB01201	Guanosine diphosphate	MetaboCard
ECMDB01397	Guanosine monophosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB02666	Thiamine monophosphate	MetaboCard
ECMDB01372	Thiamine pyrophosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
hydrolase activity

Gene Properties

Blattner:

b1134

Gene Orientation

Counterclockwise

Centisome Percentage:

25.71

Left Sequence End

1193050

Right Sequence End

1193511

Gene Sequence:

>462 bp
GTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCA
TGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAAT
GCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGAT
GTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAATGAT
GCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAG
TCAGTGCGTGAAGCCACCACGGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCA
GACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAA
CTGGAAGGAACCCAGAAGTATATTAATGAGCAGTGCAGATAG

Protein Properties

Pfam Domain Function:

NUDIX (PF00293 )

Protein Residues:

153

Protein Molecular Weight:

17433

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Phosphatase nudJ
MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETLVEAAARELWEETGISA
QPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDIDCCRWVSAEEILQASNLRS
PLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI

References

External Links:

Resource	Link
Uniprot ID:	P0AEI6
Uniprot Name:	NUDJ_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674691
Ecogene ID:	EG13446
Ecocyc:	EG13446
ColiBase:	b1134
Kegg Gene:	b1134
EchoBASE ID:	EB3220
CCDB:	NUDJ_ECOLI
BacMap:	16129097

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Lawhorn, B. G., Gerdes, S. Y., Begley, T. P. (2004). "A genetic screen for the identification of thiamin metabolic genes." J Biol Chem 279:43555-43559. Pubmed: 15292217
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
Xu, W., Dunn, C. A., O'handley, S. F., Smith, D. L., Bessman, M. J. (2006). "Three new Nudix hydrolases from Escherichia coli." J Biol Chem 281:22794-22798. Pubmed: 16766526